Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media
Introduction: Preclinical studies on mesenchymal stromal cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. In recent years, conflicting data have been reported regarding various aspects of their characteristics, development and differentiation potenti...
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2022
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| Online Access: | http://psasir.upm.edu.my/id/eprint/98109/ http://psasir.upm.edu.my/id/eprint/98109/1/2022011912061230_MJMHS_1182.pdf |
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| author | Kwan, Liang Lye Nordin, Norshariza Vidyadaran, Sharmili Niu, Jin Tan Mohd Rawi, Rohayu Izanwati Thilakavathy, Karuppiah |
| author_facet | Kwan, Liang Lye Nordin, Norshariza Vidyadaran, Sharmili Niu, Jin Tan Mohd Rawi, Rohayu Izanwati Thilakavathy, Karuppiah |
| author_sort | Kwan, Liang Lye |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: Preclinical studies on mesenchymal stromal cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. In recent years, conflicting data have been reported regarding various aspects of their characteristics, development and differentiation potential, which may be due to arrange of factors. Among the factors worth investigating is the culture medium formulation. Methods: Here we have made a comparative characterization of mouse bone marrow mesenchymal stromal cells (mBM-MSC) that were cultured using two common supplements, MesenCult™ Stimulatory Supplement (MSS) and fetal bovine serum (FBS), under the same experimental conditions at different passages. Clonogenic potential, cumulative population doubling level (CPDL), population doubling time (PDT), immunophenotyping, differentiation, immunosuppression potentials and chromosome analysis of early and late passages mBM-MSC were assessed. Results: Our findings showed that the CPDL, immunophenotype and immunosuppression potential of mBM-MSC were similar. However, variations were seen in their clonogenicity, population doubling time and differentiation efficacy whereby all of these were enhanced in DMSS. These observations suggest that their genetic make-up may be affected by both supplements upon prolonged culture. Interestingly, this conjecture was supported when chromosomal analysis revealed genetic instability of mBM-MSCs cultured in both supplements. Conclusion: In conclusion, culture medium formulation was shown to cause variations and spontaneous transformation in mBM-MSCs raising concerns on the usage of late passages mBMMSCs in fundamental and preclinical downstream experiments. |
| first_indexed | 2025-11-15T13:22:27Z |
| format | Article |
| id | upm-98109 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:22:27Z |
| publishDate | 2022 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-981092022-08-11T03:43:54Z http://psasir.upm.edu.my/id/eprint/98109/ Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media Kwan, Liang Lye Nordin, Norshariza Vidyadaran, Sharmili Niu, Jin Tan Mohd Rawi, Rohayu Izanwati Thilakavathy, Karuppiah Introduction: Preclinical studies on mesenchymal stromal cells (MSC) have allowed the cells to be considered as a promising candidate for cellular therapy. In recent years, conflicting data have been reported regarding various aspects of their characteristics, development and differentiation potential, which may be due to arrange of factors. Among the factors worth investigating is the culture medium formulation. Methods: Here we have made a comparative characterization of mouse bone marrow mesenchymal stromal cells (mBM-MSC) that were cultured using two common supplements, MesenCult™ Stimulatory Supplement (MSS) and fetal bovine serum (FBS), under the same experimental conditions at different passages. Clonogenic potential, cumulative population doubling level (CPDL), population doubling time (PDT), immunophenotyping, differentiation, immunosuppression potentials and chromosome analysis of early and late passages mBM-MSC were assessed. Results: Our findings showed that the CPDL, immunophenotype and immunosuppression potential of mBM-MSC were similar. However, variations were seen in their clonogenicity, population doubling time and differentiation efficacy whereby all of these were enhanced in DMSS. These observations suggest that their genetic make-up may be affected by both supplements upon prolonged culture. Interestingly, this conjecture was supported when chromosomal analysis revealed genetic instability of mBM-MSCs cultured in both supplements. Conclusion: In conclusion, culture medium formulation was shown to cause variations and spontaneous transformation in mBM-MSCs raising concerns on the usage of late passages mBMMSCs in fundamental and preclinical downstream experiments. Faculty of Food Science and Technology, Universiti Putra Malaysia 2022-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/98109/1/2022011912061230_MJMHS_1182.pdf Kwan, Liang Lye and Nordin, Norshariza and Vidyadaran, Sharmili and Niu, Jin Tan and Mohd Rawi, Rohayu Izanwati and Thilakavathy, Karuppiah (2022) Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media. Malaysian Journal of Medicine and Health Sciences, 18 (1). pp. 222-233. ISSN 2636-9346 https://medic.upm.edu.my/upload/dokumen/2022011912061230_MJMHS_1182.pdf |
| spellingShingle | Kwan, Liang Lye Nordin, Norshariza Vidyadaran, Sharmili Niu, Jin Tan Mohd Rawi, Rohayu Izanwati Thilakavathy, Karuppiah Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| title | Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| title_full | Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| title_fullStr | Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| title_full_unstemmed | Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| title_short | Comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| title_sort | comparative evaluation of mouse bone marrow mesenchymal stromal cells characteristics cultured in two different supplemented media |
| url | http://psasir.upm.edu.my/id/eprint/98109/ http://psasir.upm.edu.my/id/eprint/98109/ http://psasir.upm.edu.my/id/eprint/98109/1/2022011912061230_MJMHS_1182.pdf |