Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein
Japanese encephalitis virus (JEV) is the pathogen that causes Japanese encephalitis (JE) in humans and horses. Lethality of the virus was reported to be between 20–30%, of which, 30–50% of the JE survivors develop neurological and psychiatric sequelae. Attributed to the low effectiveness of current...
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| Language: | English |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/97618/ http://psasir.upm.edu.my/id/eprint/97618/1/ABSTRACT.pdf |
| _version_ | 1848862649355337728 |
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| author | Kumar, Kiven Ong, Hui Kian Tan, Wen Siang Arshad, Siti Suri Ho, Kok Lian |
| author_facet | Kumar, Kiven Ong, Hui Kian Tan, Wen Siang Arshad, Siti Suri Ho, Kok Lian |
| author_sort | Kumar, Kiven |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Japanese encephalitis virus (JEV) is the pathogen that causes Japanese encephalitis (JE) in humans and horses. Lethality of the virus was reported to be between 20–30%, of which, 30–50% of the JE survivors develop neurological and psychiatric sequelae. Attributed to the low effectiveness of current therapeutic approaches against JEV, vaccination remains the only effective approach to prevent the viral infection. Currently, live-attenuated and chimeric-live vaccines are widely used worldwide but these vaccines pose a risk of virulence restoration. Therefore, continuing development of JE vaccines with higher safety profiles and better protective efficacies is urgently needed. In this study, the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (CP) fused with the domain III of JEV envelope protein (JEV-DIII) was produced in Escherichia coli. The fusion protein (MrNV-CPJEV-DIII) assembled into virus-like particles (VLPs) with a diameter of approximately 18 nm. The BALB/c mice injected with the VLPs alone or in the presence of alum successfully elicited the production of anti-JEV-DIII antibody, with titers significantly higher than that in mice immunized with IMOJEV, a commercially available vaccine. Immunophenotyping showed that the MrNV-CPJEV-DIII supplemented with alum triggered proliferation of cytotoxic T-lymphocytes, macrophages, and natural killer (NK) cells. Additionally, cytokine profiles of the immunized mice revealed activities of cytotoxic T-lymphocytes, macrophages, and NK cells, indicating the activation of adaptive cellular and innate immune responses mediated by MrNV-CPJEV-DIII VLPs. Induction of innate, humoral, and cellular immune responses by the MrNV-CPJEV-DIII VLPs suggest that the chimeric protein is a promising JEV vaccine candidate. |
| first_indexed | 2025-11-15T13:20:23Z |
| format | Article |
| id | upm-97618 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:20:23Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-976182022-07-21T07:53:16Z http://psasir.upm.edu.my/id/eprint/97618/ Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein Kumar, Kiven Ong, Hui Kian Tan, Wen Siang Arshad, Siti Suri Ho, Kok Lian Japanese encephalitis virus (JEV) is the pathogen that causes Japanese encephalitis (JE) in humans and horses. Lethality of the virus was reported to be between 20–30%, of which, 30–50% of the JE survivors develop neurological and psychiatric sequelae. Attributed to the low effectiveness of current therapeutic approaches against JEV, vaccination remains the only effective approach to prevent the viral infection. Currently, live-attenuated and chimeric-live vaccines are widely used worldwide but these vaccines pose a risk of virulence restoration. Therefore, continuing development of JE vaccines with higher safety profiles and better protective efficacies is urgently needed. In this study, the Macrobrachium rosenbergii nodavirus (MrNV) capsid protein (CP) fused with the domain III of JEV envelope protein (JEV-DIII) was produced in Escherichia coli. The fusion protein (MrNV-CPJEV-DIII) assembled into virus-like particles (VLPs) with a diameter of approximately 18 nm. The BALB/c mice injected with the VLPs alone or in the presence of alum successfully elicited the production of anti-JEV-DIII antibody, with titers significantly higher than that in mice immunized with IMOJEV, a commercially available vaccine. Immunophenotyping showed that the MrNV-CPJEV-DIII supplemented with alum triggered proliferation of cytotoxic T-lymphocytes, macrophages, and natural killer (NK) cells. Additionally, cytokine profiles of the immunized mice revealed activities of cytotoxic T-lymphocytes, macrophages, and NK cells, indicating the activation of adaptive cellular and innate immune responses mediated by MrNV-CPJEV-DIII VLPs. Induction of innate, humoral, and cellular immune responses by the MrNV-CPJEV-DIII VLPs suggest that the chimeric protein is a promising JEV vaccine candidate. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97618/1/ABSTRACT.pdf Kumar, Kiven and Ong, Hui Kian and Tan, Wen Siang and Arshad, Siti Suri and Ho, Kok Lian (2021) Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein. Pharmaceutics, 13 (11). art. no. 1826. pp. 1-18. ISSN 1999-4923 https://www.mdpi.com/1999-4923/13/11/1826 10.3390/pharmaceutics13111826 |
| spellingShingle | Kumar, Kiven Ong, Hui Kian Tan, Wen Siang Arshad, Siti Suri Ho, Kok Lian Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein |
| title | Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein |
| title_full | Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein |
| title_fullStr | Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein |
| title_full_unstemmed | Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein |
| title_short | Immunological analysis of Nodavirus capsid displaying the domain III of Japanese Encephalitis Virus evelope protein |
| title_sort | immunological analysis of nodavirus capsid displaying the domain iii of japanese encephalitis virus evelope protein |
| url | http://psasir.upm.edu.my/id/eprint/97618/ http://psasir.upm.edu.my/id/eprint/97618/ http://psasir.upm.edu.my/id/eprint/97618/ http://psasir.upm.edu.my/id/eprint/97618/1/ABSTRACT.pdf |