Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm
Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis in the ho...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/97601/ http://psasir.upm.edu.my/id/eprint/97601/1/ABSTRACT.pdf |
| _version_ | 1848862644645134336 |
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| author | Shokrollahi, Neda Ho, Chai Ling Mohd Zainudin, Nur Ain Izzati Abdul Wahab, Mohd As'wad Wong, Mui Yun |
| author_facet | Shokrollahi, Neda Ho, Chai Ling Mohd Zainudin, Nur Ain Izzati Abdul Wahab, Mohd As'wad Wong, Mui Yun |
| author_sort | Shokrollahi, Neda |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis in the host. In this study, we aimed to isolate NRPS gene of G. boninense strain UPMGB001 and investigate the role of this gene during G. boninense-oil palm interaction. The isolated NRPS DNA fragment of 8322 bp was used to predict the putative peptide sequence of different domains and showed similarity with G. sinense (85%) at conserved motifs of three main NRPS domains. Phylogenetic analysis of NRPS peptide sequences demonstrated that NRPS of G. boninense belongs to the type VI siderophore family. The roots of 6-month-old oil palm seedlings were artificially inoculated for studying NRPS gene expression and disease severity in the greenhouse. The correlation between high disease severity (50%) and high expression (67-fold) of G. boninense NRPS gene at 4 months after inoculation and above indicated that this gene played a significant role in the advancement of BSR disease. Overall, these findings increase our knowledge on the gene structure of NRPS in G. boninense and its involvement in BSR pathogenesis as an effector gene. |
| first_indexed | 2025-11-15T13:20:18Z |
| format | Article |
| id | upm-97601 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:20:18Z |
| publishDate | 2021 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-976012022-07-25T03:06:46Z http://psasir.upm.edu.my/id/eprint/97601/ Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm Shokrollahi, Neda Ho, Chai Ling Mohd Zainudin, Nur Ain Izzati Abdul Wahab, Mohd As'wad Wong, Mui Yun Basal stem rot (BSR) of oil palm is a disastrous disease caused by a white-rot fungus Ganoderma boninense Pat. Non-ribosomal peptides (NRPs) synthesized by non-ribosomal peptide synthetases (NRPSs) are a group of secondary metabolites that act as fungal virulent factors during pathogenesis in the host. In this study, we aimed to isolate NRPS gene of G. boninense strain UPMGB001 and investigate the role of this gene during G. boninense-oil palm interaction. The isolated NRPS DNA fragment of 8322 bp was used to predict the putative peptide sequence of different domains and showed similarity with G. sinense (85%) at conserved motifs of three main NRPS domains. Phylogenetic analysis of NRPS peptide sequences demonstrated that NRPS of G. boninense belongs to the type VI siderophore family. The roots of 6-month-old oil palm seedlings were artificially inoculated for studying NRPS gene expression and disease severity in the greenhouse. The correlation between high disease severity (50%) and high expression (67-fold) of G. boninense NRPS gene at 4 months after inoculation and above indicated that this gene played a significant role in the advancement of BSR disease. Overall, these findings increase our knowledge on the gene structure of NRPS in G. boninense and its involvement in BSR pathogenesis as an effector gene. Nature Publishing Group 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97601/1/ABSTRACT.pdf Shokrollahi, Neda and Ho, Chai Ling and Mohd Zainudin, Nur Ain Izzati and Abdul Wahab, Mohd As'wad and Wong, Mui Yun (2021) Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm. Scientific Reports, 11 (1). art. no. 16330. pp. 1-16. ISSN 2045-2322 https://www.nature.com/articles/s41598-021-95549-8 10.1038/s41598-021-95549-8 |
| spellingShingle | Shokrollahi, Neda Ho, Chai Ling Mohd Zainudin, Nur Ain Izzati Abdul Wahab, Mohd As'wad Wong, Mui Yun Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm |
| title | Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm |
| title_full | Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm |
| title_fullStr | Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm |
| title_full_unstemmed | Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm |
| title_short | Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm |
| title_sort | identification of non-ribosomal peptide synthetase in ganoderma boninense pat. that was expressed during the interaction with oil palm |
| url | http://psasir.upm.edu.my/id/eprint/97601/ http://psasir.upm.edu.my/id/eprint/97601/ http://psasir.upm.edu.my/id/eprint/97601/ http://psasir.upm.edu.my/id/eprint/97601/1/ABSTRACT.pdf |