First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia

Oil palm has been contributing to the economic growth and gross national income (GNI) of Malaysia. On that note, supply of disease-free oil palm seedlings to field is crucial. At present many nurseries encounter disease infestations of the seedlings at nursery stages. One of the mo...

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Main Authors: Syd Ali, Nusaibah, Wan Nur Asmieda, Syed Sagaff, Sharifah Aliya
Format: Article
Language:English
Published: Gayathri Ashokkumar 2021
Online Access:http://psasir.upm.edu.my/id/eprint/97348/
http://psasir.upm.edu.my/id/eprint/97348/1/ABSTRACT.pdf
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author Syd Ali, Nusaibah
Wan Nur Asmieda
Syed Sagaff, Sharifah Aliya
author_facet Syd Ali, Nusaibah
Wan Nur Asmieda
Syed Sagaff, Sharifah Aliya
author_sort Syd Ali, Nusaibah
building UPM Institutional Repository
collection Online Access
description Oil palm has been contributing to the economic growth and gross national income (GNI) of Malaysia. On that note, supply of disease-free oil palm seedlings to field is crucial. At present many nurseries encounter disease infestations of the seedlings at nursery stages. One of the most common diseases identified was leaf tip blight. The disease incidences cause critical quality, quantity loss of the seedlings and draw other pathogens in the field to infect the initially infected host. Till to date, no study has been conducted to diagnose leaf tip blight disease of oil palm at nursery stage in Malaysia. Molecular identification was conducted by using 3 specific primers namely actin gene (ACT), β-tubulin-2 (Bt2), glyceraldehyde-3-phosphate dehydrogenase (GD) gene and complete rDNA-ITS (ITS) to confirm the isolates. All the isolated fungi matched 98% to 100% similarity with GenBank database. Thus, the isolated isolates were identified as Nigrosporasp., Colletotrichumgloeosporioides andPhoma sp. In vitro pathogenicity test demonstrated that,the three-isolates produced similar leaf tip blight symptoms as the infested seedlings at the nursery. As a conclusion, Nigrosporasp., C. gloeosporioidesand Phomasp.were identified as the causal pathogen of leaf tip blight of oil palm with different levels of virulence.
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spelling upm-973482022-09-12T02:00:03Z http://psasir.upm.edu.my/id/eprint/97348/ First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia Syd Ali, Nusaibah Wan Nur Asmieda Syed Sagaff, Sharifah Aliya Oil palm has been contributing to the economic growth and gross national income (GNI) of Malaysia. On that note, supply of disease-free oil palm seedlings to field is crucial. At present many nurseries encounter disease infestations of the seedlings at nursery stages. One of the most common diseases identified was leaf tip blight. The disease incidences cause critical quality, quantity loss of the seedlings and draw other pathogens in the field to infect the initially infected host. Till to date, no study has been conducted to diagnose leaf tip blight disease of oil palm at nursery stage in Malaysia. Molecular identification was conducted by using 3 specific primers namely actin gene (ACT), β-tubulin-2 (Bt2), glyceraldehyde-3-phosphate dehydrogenase (GD) gene and complete rDNA-ITS (ITS) to confirm the isolates. All the isolated fungi matched 98% to 100% similarity with GenBank database. Thus, the isolated isolates were identified as Nigrosporasp., Colletotrichumgloeosporioides andPhoma sp. In vitro pathogenicity test demonstrated that,the three-isolates produced similar leaf tip blight symptoms as the infested seedlings at the nursery. As a conclusion, Nigrosporasp., C. gloeosporioidesand Phomasp.were identified as the causal pathogen of leaf tip blight of oil palm with different levels of virulence. Gayathri Ashokkumar 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97348/1/ABSTRACT.pdf Syd Ali, Nusaibah and Wan Nur Asmieda and Syed Sagaff, Sharifah Aliya (2021) First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia. Journal of Current Opinion in Crop Science, 2 (1). 27 - 39. ISSN 2583-0392 https://jcocs.com/index.php/ej/article/view/20
spellingShingle Syd Ali, Nusaibah
Wan Nur Asmieda
Syed Sagaff, Sharifah Aliya
First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia
title First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia
title_full First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia
title_fullStr First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia
title_full_unstemmed First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia
title_short First report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in Malaysia
title_sort first report on the fungal pathogens associated with leaf tip blight of oil palm at nursery stage in malaysia
url http://psasir.upm.edu.my/id/eprint/97348/
http://psasir.upm.edu.my/id/eprint/97348/
http://psasir.upm.edu.my/id/eprint/97348/1/ABSTRACT.pdf