Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis
Loop-mediated isothermal amplification (LAMP) test is widely used in molecular diagnostics as a point-of-care technique alternative to traditional PCR especially in resource-limited countries. LAMP has been recently used to diagnose leptospirosis. Therefore, we undertook a systematic review and meta...
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| Format: | Article |
| Language: | English |
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Elsevier
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/97153/ http://psasir.upm.edu.my/id/eprint/97153/1/ABSTRACT.pdf |
| _version_ | 1848862527648169984 |
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| author | Gunasegar, Shan Neela, Vasantha Kumari |
| author_facet | Gunasegar, Shan Neela, Vasantha Kumari |
| author_sort | Gunasegar, Shan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Loop-mediated isothermal amplification (LAMP) test is widely used in molecular diagnostics as a point-of-care technique alternative to traditional PCR especially in resource-limited countries. LAMP has been recently used to diagnose leptospirosis. Therefore, we undertook a systematic review and meta-analysis to compare the accuracy of LAMP with PCR in the diagnosis of leptospirosis. Sixty-one studies were extracted from three international databases and analyzed throughout using the PRISMA guideline. The pooled sensitivity of LAMP and PCR technique was 0.80 (95% CI: 0.58–0.90) and 0.54 (95% CI: 0.35–0.67) respectively indicating that LAMP is more sensitive than PCR. The Q* value of LAMP and PCR-based technique is 274.61 and 397.95, respectively. Among the analyzed studies, significant heterogeneity was observed where I2 is 90.90% for LAMP-based and 86.18% for PCR-based. Our study suggests that LAMP has better diagnostic accuracy than PCR. However, future work should be carried out to reduce heterogeneity as well as to improve and develop effective intervention strategies. |
| first_indexed | 2025-11-15T13:18:27Z |
| format | Article |
| id | upm-97153 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:18:27Z |
| publishDate | 2021 |
| publisher | Elsevier |
| recordtype | eprints |
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| spelling | upm-971532022-09-14T01:28:14Z http://psasir.upm.edu.my/id/eprint/97153/ Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis Gunasegar, Shan Neela, Vasantha Kumari Loop-mediated isothermal amplification (LAMP) test is widely used in molecular diagnostics as a point-of-care technique alternative to traditional PCR especially in resource-limited countries. LAMP has been recently used to diagnose leptospirosis. Therefore, we undertook a systematic review and meta-analysis to compare the accuracy of LAMP with PCR in the diagnosis of leptospirosis. Sixty-one studies were extracted from three international databases and analyzed throughout using the PRISMA guideline. The pooled sensitivity of LAMP and PCR technique was 0.80 (95% CI: 0.58–0.90) and 0.54 (95% CI: 0.35–0.67) respectively indicating that LAMP is more sensitive than PCR. The Q* value of LAMP and PCR-based technique is 274.61 and 397.95, respectively. Among the analyzed studies, significant heterogeneity was observed where I2 is 90.90% for LAMP-based and 86.18% for PCR-based. Our study suggests that LAMP has better diagnostic accuracy than PCR. However, future work should be carried out to reduce heterogeneity as well as to improve and develop effective intervention strategies. Elsevier 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/97153/1/ABSTRACT.pdf Gunasegar, Shan and Neela, Vasantha Kumari (2021) Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis. Diagnostic Microbiology and Infectious Disease, 100 (3). art. no. 115369. pp. 1-11. ISSN 0732-8893 https://www.sciencedirect.com/science/article/pii/S0732889321000626 10.1016/j.diagmicrobio.2021.115369 |
| spellingShingle | Gunasegar, Shan Neela, Vasantha Kumari Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis |
| title | Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis |
| title_full | Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis |
| title_fullStr | Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis |
| title_full_unstemmed | Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis |
| title_short | Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in clinical samples: a systematic review and meta-analysis |
| title_sort | evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (lamp) compared with polymerase chain reaction (pcr) for leptospira spp. in clinical samples: a systematic review and meta-analysis |
| url | http://psasir.upm.edu.my/id/eprint/97153/ http://psasir.upm.edu.my/id/eprint/97153/ http://psasir.upm.edu.my/id/eprint/97153/ http://psasir.upm.edu.my/id/eprint/97153/1/ABSTRACT.pdf |