Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode
A new electrochemical immunosensor based on iron oxide-gold nanocomposite (Fe 3 O 4 -Au NC) as signal enhancement was developed for sensitive detection of M. tuberculosis , the causative agent of tuberculosis (TB). Reduced graphene oxide-(3-aminopropyl)-triethoxysilane (rGO-APTES) modified screen-pr...
| Main Authors: | , , , , |
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| Format: | Article |
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Institute of Electrical and Electronics Engineers
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/97099/ |
| _version_ | 1848862515294896128 |
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| author | Syed Amri, Syazana Ameera Yusof, Nor Azah Abdullah, Jaafar Abd Rahman, Siti Fatimah Mohd Azmi, Umi Zulaikha |
| author_facet | Syed Amri, Syazana Ameera Yusof, Nor Azah Abdullah, Jaafar Abd Rahman, Siti Fatimah Mohd Azmi, Umi Zulaikha |
| author_sort | Syed Amri, Syazana Ameera |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A new electrochemical immunosensor based on iron oxide-gold nanocomposite (Fe 3 O 4 -Au NC) as signal enhancement was developed for sensitive detection of M. tuberculosis , the causative agent of tuberculosis (TB). Reduced graphene oxide-(3-aminopropyl)-triethoxysilane (rGO-APTES) modified screen-printed electrode (SPE) was employed as a matrix to immobilize the capture antibodies (Ab). The detection signal was amplified due to the large amounts of Ab captured on the large surface area of modified SPE. The Fe 3 O 4 -Au NC were functionalized as label to conjugate with primary antibodies (Ab 1 ). Due to the homogeneity and narrow size distribution of Fe 3 O 4 -Au NC, several Ab 1 were bound on each nanocomposite. The introduction of Fe 3 O 4 -Au-Ab 1 onto the electrode surface through sandwich immunoreactions enhances the performance of the sensor. The proposed immunosensor offered a sensitive detection of TB in the range of 0– 9.0 μg /mL, with a detection limit of 1.32 ng/mL. A disposable and sensitive SPE-based electrochemical immunoassay would serve as a promising platform to detect various mycobacterium pathogens for future diagnostic studies. |
| first_indexed | 2025-11-15T13:18:15Z |
| format | Article |
| id | upm-97099 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:18:15Z |
| publishDate | 2021 |
| publisher | Institute of Electrical and Electronics Engineers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-970992023-04-19T04:06:02Z http://psasir.upm.edu.my/id/eprint/97099/ Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode Syed Amri, Syazana Ameera Yusof, Nor Azah Abdullah, Jaafar Abd Rahman, Siti Fatimah Mohd Azmi, Umi Zulaikha A new electrochemical immunosensor based on iron oxide-gold nanocomposite (Fe 3 O 4 -Au NC) as signal enhancement was developed for sensitive detection of M. tuberculosis , the causative agent of tuberculosis (TB). Reduced graphene oxide-(3-aminopropyl)-triethoxysilane (rGO-APTES) modified screen-printed electrode (SPE) was employed as a matrix to immobilize the capture antibodies (Ab). The detection signal was amplified due to the large amounts of Ab captured on the large surface area of modified SPE. The Fe 3 O 4 -Au NC were functionalized as label to conjugate with primary antibodies (Ab 1 ). Due to the homogeneity and narrow size distribution of Fe 3 O 4 -Au NC, several Ab 1 were bound on each nanocomposite. The introduction of Fe 3 O 4 -Au-Ab 1 onto the electrode surface through sandwich immunoreactions enhances the performance of the sensor. The proposed immunosensor offered a sensitive detection of TB in the range of 0– 9.0 μg /mL, with a detection limit of 1.32 ng/mL. A disposable and sensitive SPE-based electrochemical immunoassay would serve as a promising platform to detect various mycobacterium pathogens for future diagnostic studies. Institute of Electrical and Electronics Engineers 2021 Article PeerReviewed Syed Amri, Syazana Ameera and Yusof, Nor Azah and Abdullah, Jaafar and Abd Rahman, Siti Fatimah and Mohd Azmi, Umi Zulaikha (2021) Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode. IEEE Sensors Journal, 21 (6). 7233 - 7241. ISSN 1530-437X https://ieeexplore.ieee.org/document/9293114 10.1109/JSEN.2020.3044246 |
| spellingShingle | Syed Amri, Syazana Ameera Yusof, Nor Azah Abdullah, Jaafar Abd Rahman, Siti Fatimah Mohd Azmi, Umi Zulaikha Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode |
| title | Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode |
| title_full | Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode |
| title_fullStr | Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode |
| title_full_unstemmed | Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode |
| title_short | Enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rGO-APTES modified screen-printed electrode |
| title_sort | enhancement of electrochemical properties using iron oxide-gold nanocomposite for tuberculosis detection based on rgo-aptes modified screen-printed electrode |
| url | http://psasir.upm.edu.my/id/eprint/97099/ http://psasir.upm.edu.my/id/eprint/97099/ http://psasir.upm.edu.my/id/eprint/97099/ |