Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study
Aim: To evaluate the effects of gestational diabetes mellitus (GDM) on the quantity and quality of hematopoietic stem cells (HSC). Methods: In this case-control study, HSC were isolated from umbilical cord blood (UCB) procured at delivery from 63 mothers with GDM and 67 healthy mothers. Total nuclea...
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| Format: | Article |
| Language: | English |
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Medicinska Naklada
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96987/ http://psasir.upm.edu.my/id/eprint/96987/1/ABSTRACT.pdf |
| _version_ | 1848862488549916672 |
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| author | Tusimin, Maiza Ahmed, Sara M. El. Kai, Wei Lee Ching, Siew Mooi Md Noor, Sabariah Nordin, Norshariza |
| author_facet | Tusimin, Maiza Ahmed, Sara M. El. Kai, Wei Lee Ching, Siew Mooi Md Noor, Sabariah Nordin, Norshariza |
| author_sort | Tusimin, Maiza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aim: To evaluate the effects of gestational diabetes mellitus (GDM) on the quantity and quality of hematopoietic stem cells (HSC). Methods: In this case-control study, HSC were isolated from umbilical cord blood (UCB) procured at delivery from 63 mothers with GDM and 67 healthy mothers. Total nucleated cells (TNC) and CD34+ cells were quantified using BD FACSCalibur flow cytometer. The quantity and quality of stem cells were determined. Results: The GDM group had lower total cord blood volume and lower number of nucleated HSC compared with healthy mothers. Regarding stem cell quantity parameters, they had significantly lower UCB volume (P=0.041), TNC count (P=0.022), total viable NC count (P=0.014), and CD34+ percentage (P=0.014). Regarding the quality of stem cells, they had significantly lower viable TNC percentage (P=0.015). The predictors for total TNC count were longer labor duration (adjusted B coefficient [p]: 0.031 [0.046]), greater estimated blood loss (0.089 [0.005]), female neonates (12.322 [0.049]), and higher placenta weight (0.080 [0.033]). The predictors of total viable NC count were greater estimated blood loss (0.092 [0.003]), female neonates (13.16 [0.035]), and greater placenta weight (0.083 [0.026]). Conclusion: The GDM group had much lower quantity and quality of UCB stem cells. Our results should be taken into consideration when drawing cord blood for unrelated stem cell banking in an obstetric unit to ensure the obtaining of optimal cord blood samples and to avoid unnecessary expenses. |
| first_indexed | 2025-11-15T13:17:49Z |
| format | Article |
| id | upm-96987 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:17:49Z |
| publishDate | 2021 |
| publisher | Medicinska Naklada |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-969872022-10-18T04:42:59Z http://psasir.upm.edu.my/id/eprint/96987/ Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study Tusimin, Maiza Ahmed, Sara M. El. Kai, Wei Lee Ching, Siew Mooi Md Noor, Sabariah Nordin, Norshariza Aim: To evaluate the effects of gestational diabetes mellitus (GDM) on the quantity and quality of hematopoietic stem cells (HSC). Methods: In this case-control study, HSC were isolated from umbilical cord blood (UCB) procured at delivery from 63 mothers with GDM and 67 healthy mothers. Total nucleated cells (TNC) and CD34+ cells were quantified using BD FACSCalibur flow cytometer. The quantity and quality of stem cells were determined. Results: The GDM group had lower total cord blood volume and lower number of nucleated HSC compared with healthy mothers. Regarding stem cell quantity parameters, they had significantly lower UCB volume (P=0.041), TNC count (P=0.022), total viable NC count (P=0.014), and CD34+ percentage (P=0.014). Regarding the quality of stem cells, they had significantly lower viable TNC percentage (P=0.015). The predictors for total TNC count were longer labor duration (adjusted B coefficient [p]: 0.031 [0.046]), greater estimated blood loss (0.089 [0.005]), female neonates (12.322 [0.049]), and higher placenta weight (0.080 [0.033]). The predictors of total viable NC count were greater estimated blood loss (0.092 [0.003]), female neonates (13.16 [0.035]), and greater placenta weight (0.083 [0.026]). Conclusion: The GDM group had much lower quantity and quality of UCB stem cells. Our results should be taken into consideration when drawing cord blood for unrelated stem cell banking in an obstetric unit to ensure the obtaining of optimal cord blood samples and to avoid unnecessary expenses. Medicinska Naklada 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96987/1/ABSTRACT.pdf Tusimin, Maiza and Ahmed, Sara M. El. and Kai, Wei Lee and Ching, Siew Mooi and Md Noor, Sabariah and Nordin, Norshariza (2021) Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study. Croatian Medical Journal, 62 (6). pp. 590-597. ISSN 0353-9504; ESSN: 1332-8166 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8771235/ 10.3325/cmj.2021.62.590 |
| spellingShingle | Tusimin, Maiza Ahmed, Sara M. El. Kai, Wei Lee Ching, Siew Mooi Md Noor, Sabariah Nordin, Norshariza Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| title | Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| title_full | Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| title_fullStr | Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| title_full_unstemmed | Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| title_short | Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| title_sort | effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study |
| url | http://psasir.upm.edu.my/id/eprint/96987/ http://psasir.upm.edu.my/id/eprint/96987/ http://psasir.upm.edu.my/id/eprint/96987/ http://psasir.upm.edu.my/id/eprint/96987/1/ABSTRACT.pdf |