Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis

The present study aims to develop a hydroxyapatite (HAP) based scaffold composite for orthopaedic applications and for that, we adopt a Casein (Cs) micelle assisted synthesis route for the formation of a composite. Following the synthesis and characterization of various fluorine (2% and 5%) substitu...

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Main Authors: Jariya, S.A. Iynoon, Padmanabhan, Varun Prasath, Kulandaivelu, Ravichandran, Prakash, Natarajan, Mohammad, Faruq, Al-Lohedan, Hamad A., Paiman, Suriati, Schirhagl, Romana, Hossain, M.A. Motalib, Sagadevan, Suresh
Format: Article
Language:English
Published: Elsevier 2021
Online Access:http://psasir.upm.edu.my/id/eprint/96801/
http://psasir.upm.edu.my/id/eprint/96801/1/ABSTRACT.pdf
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author Jariya, S.A. Iynoon
Padmanabhan, Varun Prasath
Kulandaivelu, Ravichandran
Prakash, Natarajan
Mohammad, Faruq
Al-Lohedan, Hamad A.
Paiman, Suriati
Schirhagl, Romana
Hossain, M.A. Motalib
Sagadevan, Suresh
author_facet Jariya, S.A. Iynoon
Padmanabhan, Varun Prasath
Kulandaivelu, Ravichandran
Prakash, Natarajan
Mohammad, Faruq
Al-Lohedan, Hamad A.
Paiman, Suriati
Schirhagl, Romana
Hossain, M.A. Motalib
Sagadevan, Suresh
author_sort Jariya, S.A. Iynoon
building UPM Institutional Repository
collection Online Access
description The present study aims to develop a hydroxyapatite (HAP) based scaffold composite for orthopaedic applications and for that, we adopt a Casein (Cs) micelle assisted synthesis route for the formation of a composite. Following the synthesis and characterization of various fluorine (2% and 5%) substituted HAPs (FHAP), they have been tested for the release of Ciprofloxacin (CIP) drug and antimicrobial efficacy. The physicochemical characterization such as FTIR and Raman confirms the successful formation of the HAP composites. Similarly, the powder XRD and FESEM analysis have used for the confirmation of crystallinity and morphological behaviour, respectively. The elemental composition has confirmed using EDX analysis. The antimicrobial studies indicate that the 5% FHAP sample is possessing superior antifungal and antibacterial activities and the highest activity has been observed against the gram-positive bacteria (Staphylococcus aureus) with an inhibition zone of 47 mm while the gram-negative bacteria (Escherichia coli) has only 38 mm inhibition zone. The CIP drug release profile has been controlling with the Cs/5% FHAP sample. Therefore, this composite has carried out for the scaffold formation with the use of chitosan-alginate matrices. Further, characterization of chitosan-alginate/5% FHAP scaffold composite indicates porous, biodegradable, considerable water uptake and retention ability, along with the maintenance of controlled CIP drug-releasing properties. Based on the analysis, the as-synthesized chitosan-alginate/5% FHAP scaffold composite can be suitable for the biomedical and bioengineering applications of bone tissue growth and as an implant.
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spelling upm-968012022-12-01T04:00:25Z http://psasir.upm.edu.my/id/eprint/96801/ Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis Jariya, S.A. Iynoon Padmanabhan, Varun Prasath Kulandaivelu, Ravichandran Prakash, Natarajan Mohammad, Faruq Al-Lohedan, Hamad A. Paiman, Suriati Schirhagl, Romana Hossain, M.A. Motalib Sagadevan, Suresh The present study aims to develop a hydroxyapatite (HAP) based scaffold composite for orthopaedic applications and for that, we adopt a Casein (Cs) micelle assisted synthesis route for the formation of a composite. Following the synthesis and characterization of various fluorine (2% and 5%) substituted HAPs (FHAP), they have been tested for the release of Ciprofloxacin (CIP) drug and antimicrobial efficacy. The physicochemical characterization such as FTIR and Raman confirms the successful formation of the HAP composites. Similarly, the powder XRD and FESEM analysis have used for the confirmation of crystallinity and morphological behaviour, respectively. The elemental composition has confirmed using EDX analysis. The antimicrobial studies indicate that the 5% FHAP sample is possessing superior antifungal and antibacterial activities and the highest activity has been observed against the gram-positive bacteria (Staphylococcus aureus) with an inhibition zone of 47 mm while the gram-negative bacteria (Escherichia coli) has only 38 mm inhibition zone. The CIP drug release profile has been controlling with the Cs/5% FHAP sample. Therefore, this composite has carried out for the scaffold formation with the use of chitosan-alginate matrices. Further, characterization of chitosan-alginate/5% FHAP scaffold composite indicates porous, biodegradable, considerable water uptake and retention ability, along with the maintenance of controlled CIP drug-releasing properties. Based on the analysis, the as-synthesized chitosan-alginate/5% FHAP scaffold composite can be suitable for the biomedical and bioengineering applications of bone tissue growth and as an implant. Elsevier 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96801/1/ABSTRACT.pdf Jariya, S.A. Iynoon and Padmanabhan, Varun Prasath and Kulandaivelu, Ravichandran and Prakash, Natarajan and Mohammad, Faruq and Al-Lohedan, Hamad A. and Paiman, Suriati and Schirhagl, Romana and Hossain, M.A. Motalib and Sagadevan, Suresh (2021) Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis. Materials Chemistry and Physics, 272. art. no. 125019. pp. 1-11. ISSN 0254-0584 https://www.sciencedirect.com/science/article/pii/S0254058421008026 10.1016/j.matchemphys.2021.125019
spellingShingle Jariya, S.A. Iynoon
Padmanabhan, Varun Prasath
Kulandaivelu, Ravichandran
Prakash, Natarajan
Mohammad, Faruq
Al-Lohedan, Hamad A.
Paiman, Suriati
Schirhagl, Romana
Hossain, M.A. Motalib
Sagadevan, Suresh
Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis
title Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis
title_full Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis
title_fullStr Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis
title_full_unstemmed Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis
title_short Drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the Casein micelle assisted synthesis
title_sort drug delivery and antimicrobial studies of chitosan-alginate based hydroxyapatite bioscaffolds formed by the casein micelle assisted synthesis
url http://psasir.upm.edu.my/id/eprint/96801/
http://psasir.upm.edu.my/id/eprint/96801/
http://psasir.upm.edu.my/id/eprint/96801/
http://psasir.upm.edu.my/id/eprint/96801/1/ABSTRACT.pdf