Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia
Salmonella enterica subspecies enterica serovar Enteritidis is one of the major foodborne zoonotic pathogens globally. It has significantly impacted human health and global trade. In this investigation, whole-genome sequencing was employed to determine the antimicrobial resistance (AMR) pattern of a...
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| Format: | Article |
| Language: | English |
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Frontiers Media
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96749/ http://psasir.upm.edu.my/id/eprint/96749/1/ABSTRACT.pdf |
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| author | Zakaria, Zunita Hassan, Latiffah Ahmad, Norazah Amir Husin, Suraya Mohd Ali, Rohaya Sharif, Zawiyah Mohamed Sohaimi, Norfitriah Garba, Bashiru |
| author_facet | Zakaria, Zunita Hassan, Latiffah Ahmad, Norazah Amir Husin, Suraya Mohd Ali, Rohaya Sharif, Zawiyah Mohamed Sohaimi, Norfitriah Garba, Bashiru |
| author_sort | Zakaria, Zunita |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Salmonella enterica subspecies enterica serovar Enteritidis is one of the major foodborne zoonotic pathogens globally. It has significantly impacted human health and global trade. In this investigation, whole-genome sequencing was employed to determine the antimicrobial resistance (AMR) pattern of a collection of Salmonella Enteritidis isolated from humans, poultry, and food sources. The study also investigated the virulence genes profile of the isolates as well as the phylogenetic relationships among strains. Illumina NextSeq technology was used to sequence the genome of 82 Salmonella Enteritidis strains isolated over 3 years (2016-2018) in Peninsular Malaysia. The pattern of resistance showed that tetracycline had the highest frequency (37/82, 45.12%), and isolates from food samples showed the highest rate of 9/18 (50.00%), followed by human 17/35 (48.57%) and then poultry 11/29 (37.93%). The second drug with the highest resistance rate is ampicillin with 5/29 (17.24%) for poultry, 4/35 (11.43%) for human, and 0/18 (0.00%) for food isolates respectively. Similarly, a total of 19 antimicrobial resistance (AMR) genes corresponding to the nine drugs used in the disc diffusion assay were evaluated from the whole genome sequence data. The aminoglycoside resistance gene aac(6')-ly was detected in 79 of the 82 isolates (96.34%). While the phylogenetic analysis revealed distinct lineages isolated, the three sources indicating possible cross-contamination. In conclusion, the results showed that the genomic profile of Salmonella Enteritidis isolated from humans, poultry, and food samples share genetic traits, hence the need to institute measures at controlling the continuous spread of these resistant pathogens. |
| first_indexed | 2025-11-15T13:16:59Z |
| format | Article |
| id | upm-96749 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:16:59Z |
| publishDate | 2021 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-967492022-12-01T04:31:29Z http://psasir.upm.edu.my/id/eprint/96749/ Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia Zakaria, Zunita Hassan, Latiffah Ahmad, Norazah Amir Husin, Suraya Mohd Ali, Rohaya Sharif, Zawiyah Mohamed Sohaimi, Norfitriah Garba, Bashiru Salmonella enterica subspecies enterica serovar Enteritidis is one of the major foodborne zoonotic pathogens globally. It has significantly impacted human health and global trade. In this investigation, whole-genome sequencing was employed to determine the antimicrobial resistance (AMR) pattern of a collection of Salmonella Enteritidis isolated from humans, poultry, and food sources. The study also investigated the virulence genes profile of the isolates as well as the phylogenetic relationships among strains. Illumina NextSeq technology was used to sequence the genome of 82 Salmonella Enteritidis strains isolated over 3 years (2016-2018) in Peninsular Malaysia. The pattern of resistance showed that tetracycline had the highest frequency (37/82, 45.12%), and isolates from food samples showed the highest rate of 9/18 (50.00%), followed by human 17/35 (48.57%) and then poultry 11/29 (37.93%). The second drug with the highest resistance rate is ampicillin with 5/29 (17.24%) for poultry, 4/35 (11.43%) for human, and 0/18 (0.00%) for food isolates respectively. Similarly, a total of 19 antimicrobial resistance (AMR) genes corresponding to the nine drugs used in the disc diffusion assay were evaluated from the whole genome sequence data. The aminoglycoside resistance gene aac(6')-ly was detected in 79 of the 82 isolates (96.34%). While the phylogenetic analysis revealed distinct lineages isolated, the three sources indicating possible cross-contamination. In conclusion, the results showed that the genomic profile of Salmonella Enteritidis isolated from humans, poultry, and food samples share genetic traits, hence the need to institute measures at controlling the continuous spread of these resistant pathogens. Frontiers Media 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96749/1/ABSTRACT.pdf Zakaria, Zunita and Hassan, Latiffah and Ahmad, Norazah and Amir Husin, Suraya and Mohd Ali, Rohaya and Sharif, Zawiyah and Mohamed Sohaimi, Norfitriah and Garba, Bashiru (2021) Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia. Frontiers in Microbiology, 12. art. no. 652642. pp. 1-12. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2021.652642/full 10.3389/fmicb.2021.652642 |
| spellingShingle | Zakaria, Zunita Hassan, Latiffah Ahmad, Norazah Amir Husin, Suraya Mohd Ali, Rohaya Sharif, Zawiyah Mohamed Sohaimi, Norfitriah Garba, Bashiru Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia |
| title | Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia |
| title_full | Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia |
| title_fullStr | Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia |
| title_full_unstemmed | Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia |
| title_short | Discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of Salmonella isolates across the human, poultry and food materials sources in Malaysia |
| title_sort | discerning the antimicrobial resistance, virulence, and phylogenetic relatedness of salmonella isolates across the human, poultry and food materials sources in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/96749/ http://psasir.upm.edu.my/id/eprint/96749/ http://psasir.upm.edu.my/id/eprint/96749/ http://psasir.upm.edu.my/id/eprint/96749/1/ABSTRACT.pdf |