Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis
This study investigated dexamethasone-treatment, shedding routes, tissue antigen distribution, and pathology of caprine Brucellosis. Eighteen non-pregnant goats were randomly grouped into A, B, and C. Group A was administered dexamethasone for 7 days at 2 mg/kg before inoculating 0.5 mL B. melitensi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor and Francis
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96710/ http://psasir.upm.edu.my/id/eprint/96710/1/ABSTRACT.pdf |
| _version_ | 1848862426990116864 |
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| author | Tanko, Polycarp Nwunuji Mohd Yusoff, Md Sabri Emikpe, Benjamin Obukowho Mayowa, Opeyemi Onilude Abdullateef, Ajadi |
| author_facet | Tanko, Polycarp Nwunuji Mohd Yusoff, Md Sabri Emikpe, Benjamin Obukowho Mayowa, Opeyemi Onilude Abdullateef, Ajadi |
| author_sort | Tanko, Polycarp Nwunuji |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study investigated dexamethasone-treatment, shedding routes, tissue antigen distribution, and pathology of caprine Brucellosis. Eighteen non-pregnant goats were randomly grouped into A, B, and C. Group A was administered dexamethasone for 7 days at 2 mg/kg before inoculating 0.5 mL B. melitensis at 107 CFU ocularly while group B was inoculated 0.5 mL B. melitensis only, and C as control negative. Blood samples, ocular, nasal, and vaginal swabs were obtained for evaluation. Three goats were sacrificed from each group at days 21 and 42 post-inoculation (pi) and selected tissues collected for PCR, histopathology, and immunohistochemistry. Brucella melitensis was detected in the ocular swabs of group A significantly higher than group B. Shedding was prolonged in group A compared to B. The overall shedding was 22.2% in group A and 9.4% in group B. The uterus of both groups A and B revealed mild inflammation and microgranuloma, extensive necrotic lesions in lymph nodes. Liver showed multifocal necrosis predominantly in group A. Lesion scoring showed significantly higher scores in A compared to B. Strong immunostaining was observed in the liver, lungs, and spleen, predominantly at day 21 pi. This study demonstrated dexamethasone prolonged shedding, tissue antigen distribution, and pathology in dexamethasone-treated goats. |
| first_indexed | 2025-11-15T13:16:51Z |
| format | Article |
| id | upm-96710 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:16:51Z |
| publishDate | 2021 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-967102022-12-01T08:49:00Z http://psasir.upm.edu.my/id/eprint/96710/ Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis Tanko, Polycarp Nwunuji Mohd Yusoff, Md Sabri Emikpe, Benjamin Obukowho Mayowa, Opeyemi Onilude Abdullateef, Ajadi This study investigated dexamethasone-treatment, shedding routes, tissue antigen distribution, and pathology of caprine Brucellosis. Eighteen non-pregnant goats were randomly grouped into A, B, and C. Group A was administered dexamethasone for 7 days at 2 mg/kg before inoculating 0.5 mL B. melitensis at 107 CFU ocularly while group B was inoculated 0.5 mL B. melitensis only, and C as control negative. Blood samples, ocular, nasal, and vaginal swabs were obtained for evaluation. Three goats were sacrificed from each group at days 21 and 42 post-inoculation (pi) and selected tissues collected for PCR, histopathology, and immunohistochemistry. Brucella melitensis was detected in the ocular swabs of group A significantly higher than group B. Shedding was prolonged in group A compared to B. The overall shedding was 22.2% in group A and 9.4% in group B. The uterus of both groups A and B revealed mild inflammation and microgranuloma, extensive necrotic lesions in lymph nodes. Liver showed multifocal necrosis predominantly in group A. Lesion scoring showed significantly higher scores in A compared to B. Strong immunostaining was observed in the liver, lungs, and spleen, predominantly at day 21 pi. This study demonstrated dexamethasone prolonged shedding, tissue antigen distribution, and pathology in dexamethasone-treated goats. Taylor and Francis 2021 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/96710/1/ABSTRACT.pdf Tanko, Polycarp Nwunuji and Mohd Yusoff, Md Sabri and Emikpe, Benjamin Obukowho and Mayowa, Opeyemi Onilude and Abdullateef, Ajadi (2021) Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis. Journal of Immunoassay and Immunochemistry, 42 (3). 265 - 284. ISSN 1532-1819; ESSN: 1532-4230 https://www.tandfonline.com/doi/abs/10.1080/15321819.2020.1862862 10.1080/15321819.2020.1862862 |
| spellingShingle | Tanko, Polycarp Nwunuji Mohd Yusoff, Md Sabri Emikpe, Benjamin Obukowho Mayowa, Opeyemi Onilude Abdullateef, Ajadi Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis |
| title | Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis |
| title_full | Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis |
| title_fullStr | Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis |
| title_full_unstemmed | Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis |
| title_short | Dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine Brucellosis |
| title_sort | dexamethasone-induced stress exacerbates shedding, tissue antigen distribution, and pathology of caprine brucellosis |
| url | http://psasir.upm.edu.my/id/eprint/96710/ http://psasir.upm.edu.my/id/eprint/96710/ http://psasir.upm.edu.my/id/eprint/96710/ http://psasir.upm.edu.my/id/eprint/96710/1/ABSTRACT.pdf |