Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis)
Basal stem rot (BSR) disease caused by the white rot fungus Ganoderma boninense is the most destructive oil palm disease leading to production losses in fresh fruit bunches. Non-ribosomal peptide synthetase (NRPS) plays an important role in fungal pathogenicity. These large multi-modular enzymes cat...
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| Format: | Article |
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Malaysian Palm Oil Board
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96546/ |
| _version_ | 1848862392433246208 |
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| author | Chua, Jackie Kwan, Yee Min Othman, Abrizah Wong, Mui Yun |
| author_facet | Chua, Jackie Kwan, Yee Min Othman, Abrizah Wong, Mui Yun |
| author_sort | Chua, Jackie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Basal stem rot (BSR) disease caused by the white rot fungus Ganoderma boninense is the most destructive oil palm disease leading to production losses in fresh fruit bunches. Non-ribosomal peptide synthetase (NRPS) plays an important role in fungal pathogenicity. These large multi-modular enzymes catalyse the biosynthesis of secondary metabolites that act as fungal virulence factors. In this study, the detection of NRPS in G. boninense was achieved using polymerase chain reaction (PCR)-based method. Core motifs of adenylation domain of NRPS gene was identified in G. boninense. The deduced amino acid sequence showed similarity to the conserved core motifs (A2, A3 and A5) of the adenylation domain. Siderophores were predicted as the potential secondary metabolites synthesised by NRPS. Expression analysis of GbNRPS in 3-month-old oil palm artificially infected with G. boninense has confirmed the upregulation of GbNRPS at 1 month after inoculation (MAI) peaking at 4 MAI in susceptible clone but not in tolerant clone. There was a correlation between GbNRPS gene expression and disease severity. Susceptible clones showed significantly higher disease severity index (DSI) (62.50%) compared to tolerant clones (28.13%) at 4 MAI. This is the first putative detection of adenylation domain of NRPS (GbNRPS) gene and functional analysis of NRPS as a virulence factor in disease development. |
| first_indexed | 2025-11-15T13:16:18Z |
| format | Article |
| id | upm-96546 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:16:18Z |
| publishDate | 2021 |
| publisher | Malaysian Palm Oil Board |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-965462023-01-11T08:35:24Z http://psasir.upm.edu.my/id/eprint/96546/ Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) Chua, Jackie Kwan, Yee Min Othman, Abrizah Wong, Mui Yun Basal stem rot (BSR) disease caused by the white rot fungus Ganoderma boninense is the most destructive oil palm disease leading to production losses in fresh fruit bunches. Non-ribosomal peptide synthetase (NRPS) plays an important role in fungal pathogenicity. These large multi-modular enzymes catalyse the biosynthesis of secondary metabolites that act as fungal virulence factors. In this study, the detection of NRPS in G. boninense was achieved using polymerase chain reaction (PCR)-based method. Core motifs of adenylation domain of NRPS gene was identified in G. boninense. The deduced amino acid sequence showed similarity to the conserved core motifs (A2, A3 and A5) of the adenylation domain. Siderophores were predicted as the potential secondary metabolites synthesised by NRPS. Expression analysis of GbNRPS in 3-month-old oil palm artificially infected with G. boninense has confirmed the upregulation of GbNRPS at 1 month after inoculation (MAI) peaking at 4 MAI in susceptible clone but not in tolerant clone. There was a correlation between GbNRPS gene expression and disease severity. Susceptible clones showed significantly higher disease severity index (DSI) (62.50%) compared to tolerant clones (28.13%) at 4 MAI. This is the first putative detection of adenylation domain of NRPS (GbNRPS) gene and functional analysis of NRPS as a virulence factor in disease development. Malaysian Palm Oil Board 2021 Article PeerReviewed Chua, Jackie and Kwan, Yee Min and Othman, Abrizah and Wong, Mui Yun (2021) Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis). Journal of Oil Palm Research, 34 (1). pp. 1-10. ISSN 1511-2780 http://jopr.mpob.gov.my/correlation-between-non-ribosomal-peptide-synthetase-nrps-production-and-virulence-of-iganoderma-boninensei-per71-on-oil-palm-ielaeis-guineensisi/ 10.21894/jopr.2021.0025 |
| spellingShingle | Chua, Jackie Kwan, Yee Min Othman, Abrizah Wong, Mui Yun Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) |
| title | Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) |
| title_full | Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) |
| title_fullStr | Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) |
| title_full_unstemmed | Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) |
| title_short | Correlation between non-robosomal peptide synthetase (NRPS) production and virulence of Ganoderma boninense PER71 on oil palm (Elaeis guineensis) |
| title_sort | correlation between non-robosomal peptide synthetase (nrps) production and virulence of ganoderma boninense per71 on oil palm (elaeis guineensis) |
| url | http://psasir.upm.edu.my/id/eprint/96546/ http://psasir.upm.edu.my/id/eprint/96546/ http://psasir.upm.edu.my/id/eprint/96546/ |