Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development
To gain insights on protein changes in fruit setting and growth in oil palm, a comparative proteomic approach was undertaken to study proteome changes during its early development. The variations in the proteome at five early developmental stages were investigated via a gel-based proteomic technique...
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| Format: | Article |
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Elsevier
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96455/ |
| _version_ | 1848862368728088576 |
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| author | Kok, Sau Yee Namasivayam, Parameswari Ee, Gwendoline Cheng Lian Ong Abdullah, Meilina |
| author_facet | Kok, Sau Yee Namasivayam, Parameswari Ee, Gwendoline Cheng Lian Ong Abdullah, Meilina |
| author_sort | Kok, Sau Yee |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | To gain insights on protein changes in fruit setting and growth in oil palm, a comparative proteomic approach was undertaken to study proteome changes during its early development. The variations in the proteome at five early developmental stages were investigated via a gel-based proteomic technique. A total of 129 variant proteins were determined using mass spectrometric analysis, resulting in 80 identifications. The majority of the identified protein species were classified as energy and metabolism, stress response/defence and cell structure during early oil palm development representing potential candidates for the control of final fruit size and composition. Seven prominent protein species were then characterised using real-time polymerase chain reaction to validate the mRNA expression against the protein abundant profiles. Transcript and protein profiles were parallel across the developmental stages, but divergent expression was observed in one protein spot, indicative of possible post-transcriptional events. Our results revealed protein changes in early oil palm fruit development provide valuable information in the understanding of fruit growth and metabolism during early stages that may contribute towards improving agronomic traits. |
| first_indexed | 2025-11-15T13:15:55Z |
| format | Article |
| id | upm-96455 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:15:55Z |
| publishDate | 2021 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-964552023-01-11T09:02:18Z http://psasir.upm.edu.my/id/eprint/96455/ Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development Kok, Sau Yee Namasivayam, Parameswari Ee, Gwendoline Cheng Lian Ong Abdullah, Meilina To gain insights on protein changes in fruit setting and growth in oil palm, a comparative proteomic approach was undertaken to study proteome changes during its early development. The variations in the proteome at five early developmental stages were investigated via a gel-based proteomic technique. A total of 129 variant proteins were determined using mass spectrometric analysis, resulting in 80 identifications. The majority of the identified protein species were classified as energy and metabolism, stress response/defence and cell structure during early oil palm development representing potential candidates for the control of final fruit size and composition. Seven prominent protein species were then characterised using real-time polymerase chain reaction to validate the mRNA expression against the protein abundant profiles. Transcript and protein profiles were parallel across the developmental stages, but divergent expression was observed in one protein spot, indicative of possible post-transcriptional events. Our results revealed protein changes in early oil palm fruit development provide valuable information in the understanding of fruit growth and metabolism during early stages that may contribute towards improving agronomic traits. Elsevier 2021 Article PeerReviewed Kok, Sau Yee and Namasivayam, Parameswari and Ee, Gwendoline Cheng Lian and Ong Abdullah, Meilina (2021) Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development. Journal of Proteomics, 232. art. no. 104052. pp. 1-13. ISSN 1874-3919 https://www.sciencedirect.com/science/article/pii/S1874391920304206 10.1016/j.jprot.2020.104052 |
| spellingShingle | Kok, Sau Yee Namasivayam, Parameswari Ee, Gwendoline Cheng Lian Ong Abdullah, Meilina Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development |
| title | Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development |
| title_full | Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development |
| title_fullStr | Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development |
| title_full_unstemmed | Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development |
| title_short | Comparative proteomic analysis of oil palm (Elaeis guineensis Jacq.) during early fruit development |
| title_sort | comparative proteomic analysis of oil palm (elaeis guineensis jacq.) during early fruit development |
| url | http://psasir.upm.edu.my/id/eprint/96455/ http://psasir.upm.edu.my/id/eprint/96455/ http://psasir.upm.edu.my/id/eprint/96455/ |