Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)
The genus Aeromonas has been recognised as an important pathogenic species in aquaculture that causes motile Aeromonas septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different Aeromonas spp. that were previously isolated from freshwater fish with s...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96454/ |
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| author | Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Annas, Salleh Yusof, Mohd Termizi Monir, Md Shirajum Mohamad, Aslah Muhamad Sofie, Mohd Hafiz Ngoo Lee, Jing Yie Chin, Yong Kit Zahaludin, Muhammad Amir Danial Addenan, Nurul Asyiqin Basri, Lukman Liles, Mark R. Amal, Mohammad Noor Azmai |
| author_facet | Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Annas, Salleh Yusof, Mohd Termizi Monir, Md Shirajum Mohamad, Aslah Muhamad Sofie, Mohd Hafiz Ngoo Lee, Jing Yie Chin, Yong Kit Zahaludin, Muhammad Amir Danial Addenan, Nurul Asyiqin Basri, Lukman Liles, Mark R. Amal, Mohammad Noor Azmai |
| author_sort | Sayuti, Mohamad Azzam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The genus Aeromonas has been recognised as an important pathogenic species in aquaculture that causes motile Aeromonas septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different Aeromonas spp. that were previously isolated from freshwater fish with signs of MAS. A total of 124 isolates of Aeromonas spp. were initially screened for the ability to grow on M9 agar with myo-inositol as a sole carbon source, which is a discriminatory phenotype for the hypervirulent A. hydrophila (vAh) pathotype. Subsequently, LD50 of six selected Aeromonas spp. were determined by intraperitoneal injection of bacterial suspension containing 103, 105, and 107 CFU/mL of the respective Aeromonas sp. to red hybrid tilapias. The kidneys, livers and spleens of infected moribund fish were examined for histopathological changes. The screening revealed that only A. dhakensis 1P11S3 was able to grow using myo-inositol as a sole carbon source, and no vAh strains were identified. The LD50–240h of A. dhakensis 1P11S3 was 107 CFU/mL, while the non-myo-inositol utilizing A. dhakensis 4PS2 and A. hydrophila 8TK3 was lower at 105 CFU/mL. Similarly, tilapia challenged with the myo-inositol A. dhakensis 1P11S3 showed significantly (p < 0.05) less severe signs, gross and histopathological lesions, and a lower mortality rate than the non-myo-inositol A. dhakensis 4PS2 and A. hydrophila 8TK3. These findings suggested that myo-inositol utilizing A. dhakensis 1P11S3 was not a hypervirulent Aeromonas sp. under current experimental disease challenge conditions, and that diverse Aeromonas spp. are of concern in aquaculture farmed freshwater fish. Therefore, future study is warranted on genomic level to further elucidate the influence of myo-inositol utilizing ability on the pathogenesis of Aeromonas spp., since this ability correlates with hypervirulence in A. hydrophila strains. |
| first_indexed | 2025-11-15T13:15:55Z |
| format | Article |
| id | upm-96454 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:15:55Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-964542023-02-08T02:53:03Z http://psasir.upm.edu.my/id/eprint/96454/ Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Annas, Salleh Yusof, Mohd Termizi Monir, Md Shirajum Mohamad, Aslah Muhamad Sofie, Mohd Hafiz Ngoo Lee, Jing Yie Chin, Yong Kit Zahaludin, Muhammad Amir Danial Addenan, Nurul Asyiqin Basri, Lukman Liles, Mark R. Amal, Mohammad Noor Azmai The genus Aeromonas has been recognised as an important pathogenic species in aquaculture that causes motile Aeromonas septicaemia (MAS) or less severe, chronic infections. This study compares the pathogenicity of the different Aeromonas spp. that were previously isolated from freshwater fish with signs of MAS. A total of 124 isolates of Aeromonas spp. were initially screened for the ability to grow on M9 agar with myo-inositol as a sole carbon source, which is a discriminatory phenotype for the hypervirulent A. hydrophila (vAh) pathotype. Subsequently, LD50 of six selected Aeromonas spp. were determined by intraperitoneal injection of bacterial suspension containing 103, 105, and 107 CFU/mL of the respective Aeromonas sp. to red hybrid tilapias. The kidneys, livers and spleens of infected moribund fish were examined for histopathological changes. The screening revealed that only A. dhakensis 1P11S3 was able to grow using myo-inositol as a sole carbon source, and no vAh strains were identified. The LD50–240h of A. dhakensis 1P11S3 was 107 CFU/mL, while the non-myo-inositol utilizing A. dhakensis 4PS2 and A. hydrophila 8TK3 was lower at 105 CFU/mL. Similarly, tilapia challenged with the myo-inositol A. dhakensis 1P11S3 showed significantly (p < 0.05) less severe signs, gross and histopathological lesions, and a lower mortality rate than the non-myo-inositol A. dhakensis 4PS2 and A. hydrophila 8TK3. These findings suggested that myo-inositol utilizing A. dhakensis 1P11S3 was not a hypervirulent Aeromonas sp. under current experimental disease challenge conditions, and that diverse Aeromonas spp. are of concern in aquaculture farmed freshwater fish. Therefore, future study is warranted on genomic level to further elucidate the influence of myo-inositol utilizing ability on the pathogenesis of Aeromonas spp., since this ability correlates with hypervirulence in A. hydrophila strains. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Sayuti, Mohamad Azzam and Md Yasin, Ina Salwany and Saad, Mohd Zamri and Annas, Salleh and Yusof, Mohd Termizi and Monir, Md Shirajum and Mohamad, Aslah and Muhamad Sofie, Mohd Hafiz Ngoo and Lee, Jing Yie and Chin, Yong Kit and Zahaludin, Muhammad Amir Danial and Addenan, Nurul Asyiqin and Basri, Lukman and Liles, Mark R. and Amal, Mohammad Noor Azmai (2021) Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus). Biology-Basel, 10 (11). pp. 1-14. ISSN 2079-7737 https://www.mdpi.com/2079-7737/10/11/1192 10.3390/biology10111192 |
| spellingShingle | Sayuti, Mohamad Azzam Md Yasin, Ina Salwany Saad, Mohd Zamri Annas, Salleh Yusof, Mohd Termizi Monir, Md Shirajum Mohamad, Aslah Muhamad Sofie, Mohd Hafiz Ngoo Lee, Jing Yie Chin, Yong Kit Zahaludin, Muhammad Amir Danial Addenan, Nurul Asyiqin Basri, Lukman Liles, Mark R. Amal, Mohammad Noor Azmai Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) |
| title | Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) |
| title_full | Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) |
| title_fullStr | Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) |
| title_full_unstemmed | Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) |
| title_short | Comparative pathogenicity of Aeromonas spp. in cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus) |
| title_sort | comparative pathogenicity of aeromonas spp. in cultured red hybrid tilapia (oreochromis niloticus × o. mossambicus) |
| url | http://psasir.upm.edu.my/id/eprint/96454/ http://psasir.upm.edu.my/id/eprint/96454/ http://psasir.upm.edu.my/id/eprint/96454/ |