Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model
Clitorea ternatea has been used in Ayurvedic medicine as a brain stimulant to treat mental illnesses and mental functional disorders. In this study, the metabolite profiles of crude C. ternatea root extract (CTRE), ethyl acetate (EA), and 50% aqueous methanol (50% MeOH) fractions were investigated u...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/96409/ |
| _version_ | 1848862359140958208 |
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| author | Ngadni, Muhammad Afiq Akhtar, Muhammad Tayyab Ismail, Intan Safinar Norazhar, Anis Irfan Lee, Soo Yee Maulidiani, Maulidiani Shaari, Khozirah |
| author_facet | Ngadni, Muhammad Afiq Akhtar, Muhammad Tayyab Ismail, Intan Safinar Norazhar, Anis Irfan Lee, Soo Yee Maulidiani, Maulidiani Shaari, Khozirah |
| author_sort | Ngadni, Muhammad Afiq |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Clitorea ternatea has been used in Ayurvedic medicine as a brain stimulant to treat mental illnesses and mental functional disorders. In this study, the metabolite profiles of crude C. ternatea root extract (CTRE), ethyl acetate (EA), and 50% aqueous methanol (50% MeOH) fractions were investigated using ultrahigh-performance liquid chromatography–diode array detector–tandem mass spectrometry (UHPLC–DAD–MS/MS), while their effect on the stress-like behavior of zebrafish, pharmacologically induced with reserpine, was investigated. A total of 32 compounds were putatively identified, among which, a series of norneolignans, clitorienolactones, and various flavonoids (flavone, flavonol, isoflavone, and isoflavanone) was found to comprise the major constituents, particularly in the EA and 50% MeOH fractions. The clitorienolactones, presently unique to the species, were present in both the free and glycosylated forms in the roots. Both the EA and 50% MeOH fractions displayed moderate effects on the stress-induced zebrafish model, significantly decreasing freezing duration and elevating the total distance travelled and average velocity, 72 h post-treatment. The results of the present study provide further evidence that the basis for the use of C. ternatea roots in traditional medicine to alleviate brain-related conditions, such as stress and depression, is attributable to the presence of clitorienolactones and the isoflavonoidal constituents. |
| first_indexed | 2025-11-15T13:15:46Z |
| format | Article |
| id | upm-96409 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:15:46Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-964092023-02-08T07:46:06Z http://psasir.upm.edu.my/id/eprint/96409/ Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model Ngadni, Muhammad Afiq Akhtar, Muhammad Tayyab Ismail, Intan Safinar Norazhar, Anis Irfan Lee, Soo Yee Maulidiani, Maulidiani Shaari, Khozirah Clitorea ternatea has been used in Ayurvedic medicine as a brain stimulant to treat mental illnesses and mental functional disorders. In this study, the metabolite profiles of crude C. ternatea root extract (CTRE), ethyl acetate (EA), and 50% aqueous methanol (50% MeOH) fractions were investigated using ultrahigh-performance liquid chromatography–diode array detector–tandem mass spectrometry (UHPLC–DAD–MS/MS), while their effect on the stress-like behavior of zebrafish, pharmacologically induced with reserpine, was investigated. A total of 32 compounds were putatively identified, among which, a series of norneolignans, clitorienolactones, and various flavonoids (flavone, flavonol, isoflavone, and isoflavanone) was found to comprise the major constituents, particularly in the EA and 50% MeOH fractions. The clitorienolactones, presently unique to the species, were present in both the free and glycosylated forms in the roots. Both the EA and 50% MeOH fractions displayed moderate effects on the stress-induced zebrafish model, significantly decreasing freezing duration and elevating the total distance travelled and average velocity, 72 h post-treatment. The results of the present study provide further evidence that the basis for the use of C. ternatea roots in traditional medicine to alleviate brain-related conditions, such as stress and depression, is attributable to the presence of clitorienolactones and the isoflavonoidal constituents. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Ngadni, Muhammad Afiq and Akhtar, Muhammad Tayyab and Ismail, Intan Safinar and Norazhar, Anis Irfan and Lee, Soo Yee and Maulidiani, Maulidiani and Shaari, Khozirah (2021) Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model. Molecules, 26 (14). pp. 1-17. ISSN 1420-3049 https://www.mdpi.com/1420-3049/26/14/4137 10.3390/molecules26144137 |
| spellingShingle | Ngadni, Muhammad Afiq Akhtar, Muhammad Tayyab Ismail, Intan Safinar Norazhar, Anis Irfan Lee, Soo Yee Maulidiani, Maulidiani Shaari, Khozirah Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| title | Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| title_full | Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| title_fullStr | Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| title_full_unstemmed | Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| title_short | Clitorienolactones and isoflavonoids of Clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| title_sort | clitorienolactones and isoflavonoids of clitorea ternatea rootsalleviate stress-like symptoms in reserpine-induced zebrafish model |
| url | http://psasir.upm.edu.my/id/eprint/96409/ http://psasir.upm.edu.my/id/eprint/96409/ http://psasir.upm.edu.my/id/eprint/96409/ |