Antioxidant and cytotoxicity activity of Cordyceps militaris extracts against human colorectal cancer cell line
Cordyceps militaris is famous for its medicinal effects and variety of bioactivities including antimicrobial, antiinflammatory, antioxidant, immunomodulatory, or antitumor properties. The research’s objective is to look into the antioxidant and cytotoxic effects of C. militaris extract (CME) against...
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| Format: | Article |
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Journal of Applied Pharmaceutical Science
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95930/ |
| _version_ | 1848862256638459904 |
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| author | Awang, Mohd Azrie Nik Mat Daud, Nik Nurul Najihah Mohd Ismail, Nurul Izzati Poh, Guat Cheng Ismail, Mohd Fakhrulddin Ramaiya, Shiamala Devi |
| author_facet | Awang, Mohd Azrie Nik Mat Daud, Nik Nurul Najihah Mohd Ismail, Nurul Izzati Poh, Guat Cheng Ismail, Mohd Fakhrulddin Ramaiya, Shiamala Devi |
| author_sort | Awang, Mohd Azrie |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Cordyceps militaris is famous for its medicinal effects and variety of bioactivities including antimicrobial, antiinflammatory, antioxidant, immunomodulatory, or antitumor properties. The research’s objective is to look into the antioxidant and cytotoxic effects of C. militaris extract (CME) against normal human colorectal HT-29 cancer cell line. The effects of CME and fresh Cordyceps militaris (CM) on the antioxidant activities were determined using total phenolic content (TPC), total flavonoid content (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) analysis. The cytotoxic effects of various concentrations of CME on HT-29 cells were evaluated by 3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetrazolium bromide test. From the results, CME displayed strong activity of DPPH (83.8%, inhibitory concentration = 0.60 mg/ml), TPC (160 ± 0.74 mg gallic acid equivalent/100 g), and TFC (6.6 ± 1.13 mg rutin equivalent/100 g) relative to fresh CM. CME was found to be significantly more cytotoxic toward HT-29 cells with p < 0.001 in a dose-dependent manner with a cell growth inhibitory concentration of 50% of t1.53 mg/ml in contrast to cisplatin (3.11 mg/ml). The high antioxidant activities and cytotoxic effects of CME are probably due to the extract’s high phenolic and flavonoid content. According to this report, CME’s growth inhibitory activity on human HT-29 cells
is driven by an apoptotic mechanism involved in it. |
| first_indexed | 2025-11-15T13:14:08Z |
| format | Article |
| id | upm-95930 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:14:08Z |
| publishDate | 2021 |
| publisher | Journal of Applied Pharmaceutical Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-959302023-03-21T08:02:41Z http://psasir.upm.edu.my/id/eprint/95930/ Antioxidant and cytotoxicity activity of Cordyceps militaris extracts against human colorectal cancer cell line Awang, Mohd Azrie Nik Mat Daud, Nik Nurul Najihah Mohd Ismail, Nurul Izzati Poh, Guat Cheng Ismail, Mohd Fakhrulddin Ramaiya, Shiamala Devi Cordyceps militaris is famous for its medicinal effects and variety of bioactivities including antimicrobial, antiinflammatory, antioxidant, immunomodulatory, or antitumor properties. The research’s objective is to look into the antioxidant and cytotoxic effects of C. militaris extract (CME) against normal human colorectal HT-29 cancer cell line. The effects of CME and fresh Cordyceps militaris (CM) on the antioxidant activities were determined using total phenolic content (TPC), total flavonoid content (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) analysis. The cytotoxic effects of various concentrations of CME on HT-29 cells were evaluated by 3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetrazolium bromide test. From the results, CME displayed strong activity of DPPH (83.8%, inhibitory concentration = 0.60 mg/ml), TPC (160 ± 0.74 mg gallic acid equivalent/100 g), and TFC (6.6 ± 1.13 mg rutin equivalent/100 g) relative to fresh CM. CME was found to be significantly more cytotoxic toward HT-29 cells with p < 0.001 in a dose-dependent manner with a cell growth inhibitory concentration of 50% of t1.53 mg/ml in contrast to cisplatin (3.11 mg/ml). The high antioxidant activities and cytotoxic effects of CME are probably due to the extract’s high phenolic and flavonoid content. According to this report, CME’s growth inhibitory activity on human HT-29 cells is driven by an apoptotic mechanism involved in it. Journal of Applied Pharmaceutical Science 2021 Article PeerReviewed Awang, Mohd Azrie and Nik Mat Daud, Nik Nurul Najihah and Mohd Ismail, Nurul Izzati and Poh, Guat Cheng and Ismail, Mohd Fakhrulddin and Ramaiya, Shiamala Devi (2021) Antioxidant and cytotoxicity activity of Cordyceps militaris extracts against human colorectal cancer cell line. Journal of Applied Pharmaceutical Science, 11 (7). 105 - 109. ISSN 2231-3354 https://www.japsonline.com/ 10.7324/JAPS.2021.110711 |
| spellingShingle | Awang, Mohd Azrie Nik Mat Daud, Nik Nurul Najihah Mohd Ismail, Nurul Izzati Poh, Guat Cheng Ismail, Mohd Fakhrulddin Ramaiya, Shiamala Devi Antioxidant and cytotoxicity activity of Cordyceps militaris extracts against human colorectal cancer cell line |
| title | Antioxidant and cytotoxicity activity of Cordyceps militaris extracts
against human colorectal cancer cell line |
| title_full | Antioxidant and cytotoxicity activity of Cordyceps militaris extracts
against human colorectal cancer cell line |
| title_fullStr | Antioxidant and cytotoxicity activity of Cordyceps militaris extracts
against human colorectal cancer cell line |
| title_full_unstemmed | Antioxidant and cytotoxicity activity of Cordyceps militaris extracts
against human colorectal cancer cell line |
| title_short | Antioxidant and cytotoxicity activity of Cordyceps militaris extracts
against human colorectal cancer cell line |
| title_sort | antioxidant and cytotoxicity activity of cordyceps militaris extracts
against human colorectal cancer cell line |
| url | http://psasir.upm.edu.my/id/eprint/95930/ http://psasir.upm.edu.my/id/eprint/95930/ http://psasir.upm.edu.my/id/eprint/95930/ |