Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line
Introduction: Punica granatum is a fruit-bearing deciduous shrub or a small tree belonging to the family of L. Punicaceae. Pharmacological activities of P. granatum include antioxidant, anticarcinogenic, and anti-inflammatory properties. The purpose of this study is to investigate the antioxidant an...
| Main Authors: | , , , , , |
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| Format: | Article |
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Universiti Putra Malaysia
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95929/ |
| _version_ | 1848862256388898816 |
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| author | Imam Syafii, Raudhatul Falah Hassan, Masriana Azmi, Wafi Mardhiah Zailan, Nur Fatin Zalikha Ismail, Rozila Abdul Rahman, Shamima |
| author_facet | Imam Syafii, Raudhatul Falah Hassan, Masriana Azmi, Wafi Mardhiah Zailan, Nur Fatin Zalikha Ismail, Rozila Abdul Rahman, Shamima |
| author_sort | Imam Syafii, Raudhatul Falah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction: Punica granatum is a fruit-bearing deciduous shrub or a small tree belonging to the family of L. Punicaceae. Pharmacological activities of P. granatum include antioxidant, anticarcinogenic, and anti-inflammatory properties. The purpose of this study is to investigate the antioxidant and anti-inflammatory properties of P. granatum methanolic extract (PGME) in the BV2 microglial cell line. Method: The bioactive component from P. granatum peel was extracted by a standard methanolic extraction protocol. The antioxidant properties of PGME were analyzed by
total phenolic content (TPC) assay, 1,1-dipheyl-2-pycrylhydrazyl (DPPH) free radical scavenging assay, and Ferric Reducing Antioxidant Power (FRAP). The cytotoxicity activity of PGME on BV2 cells was determined by a viability test using MTS reagent. Analyses of the production of inflammatory mediators, including nitric oxide (NO) and cytokines (tumor necrosis factor-alpha [TNF-α], Interleukin [IL]-6 and monocyte chemoattractant protein [MCP]-1) have been carried out to evaluate the anti-inflammatory activity of PGME in LPS-stimulated BV2 cell line. Result: The TPC and radical scavenging activity of PGME increased in a concentration-dependent manner. However, the ferric reducing ability of PGME was slightly lower and no significant difference compared to the reference standard. PGME treatment on the LPS-stimulated BV2 cells significantly inhibits the production of NO and TNF-α and slightly reduced IL-6 level. Conclusion: PGME possesses antioxidative and anti-inflammatory properties. The PGME demonstrated anti-inflammatory activity through inhibition of pro-inflammatory immune mediators on the BV2 microglial cell line.
Therefore, we suggest that PGME potentially inhibits oxidative and inflammatory processes. |
| first_indexed | 2025-11-15T13:14:08Z |
| format | Article |
| id | upm-95929 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:14:08Z |
| publishDate | 2021 |
| publisher | Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-959292023-03-21T07:55:24Z http://psasir.upm.edu.my/id/eprint/95929/ Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line Imam Syafii, Raudhatul Falah Hassan, Masriana Azmi, Wafi Mardhiah Zailan, Nur Fatin Zalikha Ismail, Rozila Abdul Rahman, Shamima Introduction: Punica granatum is a fruit-bearing deciduous shrub or a small tree belonging to the family of L. Punicaceae. Pharmacological activities of P. granatum include antioxidant, anticarcinogenic, and anti-inflammatory properties. The purpose of this study is to investigate the antioxidant and anti-inflammatory properties of P. granatum methanolic extract (PGME) in the BV2 microglial cell line. Method: The bioactive component from P. granatum peel was extracted by a standard methanolic extraction protocol. The antioxidant properties of PGME were analyzed by total phenolic content (TPC) assay, 1,1-dipheyl-2-pycrylhydrazyl (DPPH) free radical scavenging assay, and Ferric Reducing Antioxidant Power (FRAP). The cytotoxicity activity of PGME on BV2 cells was determined by a viability test using MTS reagent. Analyses of the production of inflammatory mediators, including nitric oxide (NO) and cytokines (tumor necrosis factor-alpha [TNF-α], Interleukin [IL]-6 and monocyte chemoattractant protein [MCP]-1) have been carried out to evaluate the anti-inflammatory activity of PGME in LPS-stimulated BV2 cell line. Result: The TPC and radical scavenging activity of PGME increased in a concentration-dependent manner. However, the ferric reducing ability of PGME was slightly lower and no significant difference compared to the reference standard. PGME treatment on the LPS-stimulated BV2 cells significantly inhibits the production of NO and TNF-α and slightly reduced IL-6 level. Conclusion: PGME possesses antioxidative and anti-inflammatory properties. The PGME demonstrated anti-inflammatory activity through inhibition of pro-inflammatory immune mediators on the BV2 microglial cell line. Therefore, we suggest that PGME potentially inhibits oxidative and inflammatory processes. Universiti Putra Malaysia 2021 Article PeerReviewed Imam Syafii, Raudhatul Falah and Hassan, Masriana and Azmi, Wafi Mardhiah and Zailan, Nur Fatin Zalikha and Ismail, Rozila and Abdul Rahman, Shamima (2021) Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line. Malaysian Journal of Medicine and Health Sciences, 17 (suppl.10). 32 - 38. ISSN 2636-9346 https://medic.upm.edu.my/jurnal_kami/volume_17_2021/mjmhs_vol17_supp_10_december_2021-64369 |
| spellingShingle | Imam Syafii, Raudhatul Falah Hassan, Masriana Azmi, Wafi Mardhiah Zailan, Nur Fatin Zalikha Ismail, Rozila Abdul Rahman, Shamima Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line |
| title | Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line |
| title_full | Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line |
| title_fullStr | Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line |
| title_full_unstemmed | Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line |
| title_short | Antioxidant and anti-inflammatory properties of Punica granatum peel methanolic extract in BV2 microglial cell line |
| title_sort | antioxidant and anti-inflammatory properties of punica granatum peel methanolic extract in bv2 microglial cell line |
| url | http://psasir.upm.edu.my/id/eprint/95929/ http://psasir.upm.edu.my/id/eprint/95929/ |