Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the pi...
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| Format: | Article |
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Nature Research
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95922/ |
| _version_ | 1848862254159626240 |
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| author | Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdul Rahman Masri, Siti Norbaya Mohd Taib, Niazlin Suhaili, Zarizal Sulaiman, Nurshahira Zainal Baharin, Nurul Hana Cheah, Yun Shuan Ariffin, Zarina A. Rahman, Nor Iza Mohd Rani, Farahiyah Palanisamy, Navindra Kumari Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina |
| author_facet | Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdul Rahman Masri, Siti Norbaya Mohd Taib, Niazlin Suhaili, Zarizal Sulaiman, Nurshahira Zainal Baharin, Nurul Hana Cheah, Yun Shuan Ariffin, Zarina A. Rahman, Nor Iza Mohd Rani, Farahiyah Palanisamy, Navindra Kumari Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina |
| author_sort | Dzaraly, Nurul Diana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline (37%), and trimethoprim-sulfamethoxazole (24%). Piliated isolates occurred in a proportion of 19%; 47.3% of them were multidrug-resistant (MDR) and a majority had serotype 19F. This study showed ST236 was the most predominant sequence type (ST) among piliated isolates, which was related to PMEN clone Taiwan19F-14 (CC271). In the phylogenetic analysis, the piliated isolates were grouped into three major clades supported with 100% bootstrap values. Most piliated isolates belonged to internationally disseminated clones of S. pneumoniae, but pneumococcal conjugate vaccines (PCVs) have the potential to control them. |
| first_indexed | 2025-11-15T13:14:06Z |
| format | Article |
| id | upm-95922 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:14:06Z |
| publishDate | 2021 |
| publisher | Nature Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-959222023-03-21T04:39:26Z http://psasir.upm.edu.my/id/eprint/95922/ Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdul Rahman Masri, Siti Norbaya Mohd Taib, Niazlin Suhaili, Zarizal Sulaiman, Nurshahira Zainal Baharin, Nurul Hana Cheah, Yun Shuan Ariffin, Zarina A. Rahman, Nor Iza Mohd Rani, Farahiyah Palanisamy, Navindra Kumari Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline (37%), and trimethoprim-sulfamethoxazole (24%). Piliated isolates occurred in a proportion of 19%; 47.3% of them were multidrug-resistant (MDR) and a majority had serotype 19F. This study showed ST236 was the most predominant sequence type (ST) among piliated isolates, which was related to PMEN clone Taiwan19F-14 (CC271). In the phylogenetic analysis, the piliated isolates were grouped into three major clades supported with 100% bootstrap values. Most piliated isolates belonged to internationally disseminated clones of S. pneumoniae, but pneumococcal conjugate vaccines (PCVs) have the potential to control them. Nature Research 2021 Article PeerReviewed Dzaraly, Nurul Diana and Mohd Desa, Mohd Nasir and Muthanna, Abdul Rahman and Masri, Siti Norbaya and Mohd Taib, Niazlin and Suhaili, Zarizal and Sulaiman, Nurshahira and Zainal Baharin, Nurul Hana and Cheah, Yun Shuan and Ariffin, Zarina and A. Rahman, Nor Iza and Mohd Rani, Farahiyah and Palanisamy, Navindra Kumari and Tuan Soh, Tuan Suhaila and Abdullah, Fatimah Haslina (2021) Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia. Scientific Reports, 11. art. no. 8220. pp. 1-10. ISSN 2045-2322 https://www.nature.com/articles/s41598-021-87428-z 10.1038/s41598-021-87428-z |
| spellingShingle | Dzaraly, Nurul Diana Mohd Desa, Mohd Nasir Muthanna, Abdul Rahman Masri, Siti Norbaya Mohd Taib, Niazlin Suhaili, Zarizal Sulaiman, Nurshahira Zainal Baharin, Nurul Hana Cheah, Yun Shuan Ariffin, Zarina A. Rahman, Nor Iza Mohd Rani, Farahiyah Palanisamy, Navindra Kumari Tuan Soh, Tuan Suhaila Abdullah, Fatimah Haslina Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia |
| title | Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia |
| title_full | Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia |
| title_fullStr | Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia |
| title_full_unstemmed | Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia |
| title_short | Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia |
| title_sort | antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, peninsular malaysia |
| url | http://psasir.upm.edu.my/id/eprint/95922/ http://psasir.upm.edu.my/id/eprint/95922/ http://psasir.upm.edu.my/id/eprint/95922/ |