Anti-inflammatory activity of raw and processed stingless bee honey
The anti-inflammatory activity of raw and processed Kelulut stingless bee honey was investigated for its ability to inhibit nitric oxide (NO) production in lipopolysaccharidestimulated RAW 264.7 cells. Raw honey was optimally processed by thermal processing and thermosonication at 90°C. The 3-(4,5-...
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| Format: | Article |
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Rynnye Lyan Resources
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95911/ |
| _version_ | 1848862250512678912 |
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| author | Chong, Kar Yeen Chin, Nyuk Ling Yusof, Yus Aniza Fakurazi, Sharida |
| author_facet | Chong, Kar Yeen Chin, Nyuk Ling Yusof, Yus Aniza Fakurazi, Sharida |
| author_sort | Chong, Kar Yeen |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The anti-inflammatory activity of raw and processed Kelulut stingless bee honey was investigated for its ability to inhibit nitric oxide (NO) production in lipopolysaccharidestimulated RAW 264.7 cells. Raw honey was optimally processed by thermal processing
and thermosonication at 90°C. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) cell viability assay showed that Kelulut honey from 7.8 to 500 µg/mL was not cytotoxic to RAW 264.7 cells as it resulted in at least 80% viable cells after 24 hr.
Both raw and processed honey from 10 to 300 µg/mL displayed an increase and decrease in NO concentrations, suggesting a mixed effect of NO inhibition and enhancement. Maximum NO inhibition of 17.5% was recorded from 20 µg/mL of thermally processed
honey while the highest NO enhancement of 7.8% was from 10 µg/mL of thermosonicated honey. The NO effects were independent of honey concentration and processing techniques, suggesting its potential robustness in medicinal properties as part
of the diet. |
| first_indexed | 2025-11-15T13:14:02Z |
| format | Article |
| id | upm-95911 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:14:02Z |
| publishDate | 2021 |
| publisher | Rynnye Lyan Resources |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-959112023-03-17T03:24:52Z http://psasir.upm.edu.my/id/eprint/95911/ Anti-inflammatory activity of raw and processed stingless bee honey Chong, Kar Yeen Chin, Nyuk Ling Yusof, Yus Aniza Fakurazi, Sharida The anti-inflammatory activity of raw and processed Kelulut stingless bee honey was investigated for its ability to inhibit nitric oxide (NO) production in lipopolysaccharidestimulated RAW 264.7 cells. Raw honey was optimally processed by thermal processing and thermosonication at 90°C. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) cell viability assay showed that Kelulut honey from 7.8 to 500 µg/mL was not cytotoxic to RAW 264.7 cells as it resulted in at least 80% viable cells after 24 hr. Both raw and processed honey from 10 to 300 µg/mL displayed an increase and decrease in NO concentrations, suggesting a mixed effect of NO inhibition and enhancement. Maximum NO inhibition of 17.5% was recorded from 20 µg/mL of thermally processed honey while the highest NO enhancement of 7.8% was from 10 µg/mL of thermosonicated honey. The NO effects were independent of honey concentration and processing techniques, suggesting its potential robustness in medicinal properties as part of the diet. Rynnye Lyan Resources 2021 Article PeerReviewed Chong, Kar Yeen and Chin, Nyuk Ling and Yusof, Yus Aniza and Fakurazi, Sharida (2021) Anti-inflammatory activity of raw and processed stingless bee honey. Food Research, 5 (suppl.1). 201 - 208. ISSN 2550-2166 https://www.myfoodresearch.com/ 10.26656/fr.2017.5(S1).007 |
| spellingShingle | Chong, Kar Yeen Chin, Nyuk Ling Yusof, Yus Aniza Fakurazi, Sharida Anti-inflammatory activity of raw and processed stingless bee honey |
| title | Anti-inflammatory activity of raw and processed stingless bee honey |
| title_full | Anti-inflammatory activity of raw and processed stingless bee honey |
| title_fullStr | Anti-inflammatory activity of raw and processed stingless bee honey |
| title_full_unstemmed | Anti-inflammatory activity of raw and processed stingless bee honey |
| title_short | Anti-inflammatory activity of raw and processed stingless bee honey |
| title_sort | anti-inflammatory activity of raw and processed stingless bee honey |
| url | http://psasir.upm.edu.my/id/eprint/95911/ http://psasir.upm.edu.my/id/eprint/95911/ http://psasir.upm.edu.my/id/eprint/95911/ |