Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells
Background: This study reports on the cytotoxic properties of nordamnacanthal and damnacanthal, isolated from roots of Morinda elliptica on T-lymphoblastic leukaemia (CEM-SS) cell lines. Methods: MTT assay, DNA fragmentation, ELISA and cell cycle analysis were carried out. Results: Nordamnacanthal a...
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95902/ |
| _version_ | 1848862248131362816 |
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| author | Saiful Yazan, Latifah Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Lajis, Nordin |
| author_facet | Saiful Yazan, Latifah Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Lajis, Nordin |
| author_sort | Saiful Yazan, Latifah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: This study reports on the cytotoxic properties of nordamnacanthal and damnacanthal, isolated from roots of Morinda elliptica on T-lymphoblastic leukaemia (CEM-SS) cell lines. Methods: MTT assay, DNA fragmentation, ELISA and cell cycle analysis were carried out. Results: Nordamnacanthal and damnacanthal at IC50 values of 1.7 μg/mL and10 μg/mL, respectively. At the molecular level, these compounds caused internucleosomal DNA cleavage producing multiple 180–200 bp fragments that are visible as a “ladder” on the agarose gel. This was due to the activation of the Mg2+/Ca2+-dependent endonuclease. The induction of apoptosis by nordamnacanthal was different from the one induced by damnacanthal, in a way that it occurs independently of ongoing transcription process. Nevertheless, in both cases, the process of dephosphorylation of protein phosphates 1 and 2A, the ongoing protein synthesis and the elevations of the cytosolic Ca2+ concentration were not needed for apoptosis to take place. Nordamnacanthal was found to have a cytotoxic effect by inducing apoptosis, while damnacanthal caused arrest at the G0/G1 phase of the cell cycle. Conclusion: Damnacanthal and nordamnacanthal have anticancer properties, and could act as potential treatment for T-lymphoblastic leukemia. |
| first_indexed | 2025-11-15T13:14:00Z |
| format | Article |
| id | upm-95902 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:14:00Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-959022023-03-27T03:35:09Z http://psasir.upm.edu.my/id/eprint/95902/ Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells Saiful Yazan, Latifah Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Lajis, Nordin Background: This study reports on the cytotoxic properties of nordamnacanthal and damnacanthal, isolated from roots of Morinda elliptica on T-lymphoblastic leukaemia (CEM-SS) cell lines. Methods: MTT assay, DNA fragmentation, ELISA and cell cycle analysis were carried out. Results: Nordamnacanthal and damnacanthal at IC50 values of 1.7 μg/mL and10 μg/mL, respectively. At the molecular level, these compounds caused internucleosomal DNA cleavage producing multiple 180–200 bp fragments that are visible as a “ladder” on the agarose gel. This was due to the activation of the Mg2+/Ca2+-dependent endonuclease. The induction of apoptosis by nordamnacanthal was different from the one induced by damnacanthal, in a way that it occurs independently of ongoing transcription process. Nevertheless, in both cases, the process of dephosphorylation of protein phosphates 1 and 2A, the ongoing protein synthesis and the elevations of the cytosolic Ca2+ concentration were not needed for apoptosis to take place. Nordamnacanthal was found to have a cytotoxic effect by inducing apoptosis, while damnacanthal caused arrest at the G0/G1 phase of the cell cycle. Conclusion: Damnacanthal and nordamnacanthal have anticancer properties, and could act as potential treatment for T-lymphoblastic leukemia. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Saiful Yazan, Latifah and Gopalsamy, Banulata and Abdul Rahim, Raha and Ali, Abdul Manaf and Lajis, Nordin (2021) Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells. Molecules, 26 (6). art. no. 1554. pp. 1-20. ISSN 1420-3049 https://www.mdpi.com/1420-3049/26/6/1554 10.3390/molecules26061554 |
| spellingShingle | Saiful Yazan, Latifah Gopalsamy, Banulata Abdul Rahim, Raha Ali, Abdul Manaf Lajis, Nordin Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells |
| title | Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells |
| title_full | Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells |
| title_fullStr | Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells |
| title_full_unstemmed | Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells |
| title_short | Anticancer potential of Damnacanthal and Nordamnacanthal from Morinda elliptica roots on T-lymphoblastic leukemia cells |
| title_sort | anticancer potential of damnacanthal and nordamnacanthal from morinda elliptica roots on t-lymphoblastic leukemia cells |
| url | http://psasir.upm.edu.my/id/eprint/95902/ http://psasir.upm.edu.my/id/eprint/95902/ http://psasir.upm.edu.my/id/eprint/95902/ |