An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies
This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Multidisciplinary Digital Publishing Institute
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95871/ |
| _version_ | 1848862240535478272 |
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| author | Omar, Nur Alia Sheh Yap, Wing Fen Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Mustapha Kamil, Yasmin Mahdi, Mohd Adzir |
| author_facet | Omar, Nur Alia Sheh Yap, Wing Fen Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Mustapha Kamil, Yasmin Mahdi, Mohd Adzir |
| author_sort | Omar, Nur Alia Sheh |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins. |
| first_indexed | 2025-11-15T13:13:53Z |
| format | Article |
| id | upm-95871 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:13:53Z |
| publishDate | 2021 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-958712023-03-22T08:10:49Z http://psasir.upm.edu.my/id/eprint/95871/ An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies Omar, Nur Alia Sheh Yap, Wing Fen Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Mustapha Kamil, Yasmin Mahdi, Mohd Adzir This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins. Multidisciplinary Digital Publishing Institute 2021 Article PeerReviewed Omar, Nur Alia Sheh and Yap, Wing Fen and Ramli, Irmawati and Sadrolhosseini, Amir Reza and Abdullah, Jaafar and Yusof, Nor Azah and Mustapha Kamil, Yasmin and Mahdi, Mohd Adzir (2021) An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies. Polymers, 13 (5). art. no. 762. pp. 1-14. ISSN 2073-4360 https://www.mdpi.com/2073-4360/13/5/762 10.3390/polym13050762 |
| spellingShingle | Omar, Nur Alia Sheh Yap, Wing Fen Ramli, Irmawati Sadrolhosseini, Amir Reza Abdullah, Jaafar Yusof, Nor Azah Mustapha Kamil, Yasmin Mahdi, Mohd Adzir An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| title | An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| title_full | An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| title_fullStr | An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| title_full_unstemmed | An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| title_short | An optical sensor for dengue envelope proteins using Polyamidoamine Dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| title_sort | optical sensor for dengue envelope proteins using polyamidoamine dendrimer biopolymer-based nanocomposite thin film: enhanced sensitivity, selectivity, and recovery studies |
| url | http://psasir.upm.edu.my/id/eprint/95871/ http://psasir.upm.edu.my/id/eprint/95871/ http://psasir.upm.edu.my/id/eprint/95871/ |