Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia
Background: Type 2 Diabetes mellitus (T2DM) is a chronic metabolic disorder. It is a major non-communicable disease affecting 463 million people globally in 2019 and is expected to be double to about 700 million by 2045. The majority are Asians with Indian ethnicity in Malaysia reported as the highe...
| Main Authors: | , , , , , |
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| Format: | Article |
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Elsevier
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95797/ |
| _version_ | 1848862222804058112 |
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| author | Al-Ashoor, Sabah Ghasan Abood Ramachandran, Vasudevan Inche Mat, Liyana Najwa Mohamad, Nur Afiqah Mohamed, Mohd Hazmi Wan Sulaiman, Wan Aliaa |
| author_facet | Al-Ashoor, Sabah Ghasan Abood Ramachandran, Vasudevan Inche Mat, Liyana Najwa Mohamad, Nur Afiqah Mohamed, Mohd Hazmi Wan Sulaiman, Wan Aliaa |
| author_sort | Al-Ashoor, Sabah Ghasan Abood |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Type 2 Diabetes mellitus (T2DM) is a chronic metabolic disorder. It is a major non-communicable disease affecting 463 million people globally in 2019 and is expected to be double to about 700 million by 2045. The majority are Asians with Indian ethnicity in Malaysia reported as the highest prevalence of T2DM. Cardiovascular disease, renal failure, blindness and neuropathy, as well as premature death are the known morbidity and mortality resulted from T2DM. T2DM is characterized by the dysfunctional insulin physiology that causes reduction of glucose transport into the cells which lead to hyperglycaemia. Hence, one of the important treatments is an oral antidiabetic drug that lowers the serum glucose level in patients with T2DM. This drug will be transported across cell membranes by organic cation transporters (OCT). Therefore, it is important to identify the OCT candidate gene polymorphisms related to T2DM especially among the Indian ethnicity in Malaysia. Methods: Blood samples were collected from 132 T2DM patients and 133 controls. Genotyping of OCT1 (rs628031), OCT2 (rs145450955), OCT3 (rs3088442 and rs2292334) was performed using (PCR-RFLP). Results: No association was observed for genotypic and allelic distributions in all the gene polymorphisms of OCT genes (P > 0.05). However, a logistic regression analysis stratified by gender in a dominant model showed a significant difference for OCT3 among males with T2DM (P = 0.006). Significant association was also observed for OCT3 when stratified to subjects aged > 45 years old (P = 0.009). Conclusion: Based on these findings, the association of OCT3 (rs2292334) could be considered as a possible genetic risk factor for the development of T2DM among Indian males alone. |
| first_indexed | 2025-11-15T13:13:36Z |
| format | Article |
| id | upm-95797 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:13:36Z |
| publishDate | 2021 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-957972023-03-31T02:44:34Z http://psasir.upm.edu.my/id/eprint/95797/ Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia Al-Ashoor, Sabah Ghasan Abood Ramachandran, Vasudevan Inche Mat, Liyana Najwa Mohamad, Nur Afiqah Mohamed, Mohd Hazmi Wan Sulaiman, Wan Aliaa Background: Type 2 Diabetes mellitus (T2DM) is a chronic metabolic disorder. It is a major non-communicable disease affecting 463 million people globally in 2019 and is expected to be double to about 700 million by 2045. The majority are Asians with Indian ethnicity in Malaysia reported as the highest prevalence of T2DM. Cardiovascular disease, renal failure, blindness and neuropathy, as well as premature death are the known morbidity and mortality resulted from T2DM. T2DM is characterized by the dysfunctional insulin physiology that causes reduction of glucose transport into the cells which lead to hyperglycaemia. Hence, one of the important treatments is an oral antidiabetic drug that lowers the serum glucose level in patients with T2DM. This drug will be transported across cell membranes by organic cation transporters (OCT). Therefore, it is important to identify the OCT candidate gene polymorphisms related to T2DM especially among the Indian ethnicity in Malaysia. Methods: Blood samples were collected from 132 T2DM patients and 133 controls. Genotyping of OCT1 (rs628031), OCT2 (rs145450955), OCT3 (rs3088442 and rs2292334) was performed using (PCR-RFLP). Results: No association was observed for genotypic and allelic distributions in all the gene polymorphisms of OCT genes (P > 0.05). However, a logistic regression analysis stratified by gender in a dominant model showed a significant difference for OCT3 among males with T2DM (P = 0.006). Significant association was also observed for OCT3 when stratified to subjects aged > 45 years old (P = 0.009). Conclusion: Based on these findings, the association of OCT3 (rs2292334) could be considered as a possible genetic risk factor for the development of T2DM among Indian males alone. Elsevier 2021 Article PeerReviewed Al-Ashoor, Sabah Ghasan Abood and Ramachandran, Vasudevan and Inche Mat, Liyana Najwa and Mohamad, Nur Afiqah and Mohamed, Mohd Hazmi and Wan Sulaiman, Wan Aliaa (2021) Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia. Saudi Journal of Biological Sciences, 29 (1). pp. 453-459. ISSN 1319-562X https://www.sciencedirect.com/science/article/pii/S1319562X21008020 10.1016/j.sjbs.2021.09.008 |
| spellingShingle | Al-Ashoor, Sabah Ghasan Abood Ramachandran, Vasudevan Inche Mat, Liyana Najwa Mohamad, Nur Afiqah Mohamed, Mohd Hazmi Wan Sulaiman, Wan Aliaa Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia |
| title | Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia |
| title_full | Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia |
| title_fullStr | Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia |
| title_full_unstemmed | Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia |
| title_short | Analysis of OCT1, OCT2 and OCT3 gene polymorphisms among type 2 diabetes mellitus subjects in Indian ethnicity, Malaysia |
| title_sort | analysis of oct1, oct2 and oct3 gene polymorphisms among type 2 diabetes mellitus subjects in indian ethnicity, malaysia |
| url | http://psasir.upm.edu.my/id/eprint/95797/ http://psasir.upm.edu.my/id/eprint/95797/ http://psasir.upm.edu.my/id/eprint/95797/ |