The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides
Chitosan is a source of potential bioactive material with many uses in the food, health and agricultural sectors. However, the limitation to utilize the chitosan in biological applications, comprising poor solubility under physiological conditions. In order to overcome the limitations, chitosan is b...
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| Format: | Article |
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Innovative Scientific Information and Services Network
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95384/ |
| _version_ | 1848862145515618304 |
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| author | Nurhayati, Yusof Mohamad Azman, Ezzat Abd. Ghani, Asmaliza Yusof, Norzaida Tang, John Yew Huat |
| author_facet | Nurhayati, Yusof Mohamad Azman, Ezzat Abd. Ghani, Asmaliza Yusof, Norzaida Tang, John Yew Huat |
| author_sort | Nurhayati, Yusof |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Chitosan is a source of potential bioactive material with many uses in the food, health and agricultural sectors. However, the limitation to utilize the chitosan in biological applications, comprising poor solubility under physiological conditions. In order to overcome the limitations, chitosan is being partially hydrolysed to produce chitosan oligosaccharides (COS). The objective of this research was to determine the degree of deacetylation (DDA), solubility and viscosity of the chitosan and COS. In this study, chitosan was hydrolysed by the cellulase from Trichoderma reesei to produce COS. The DDA of chitosan and COS were determined by linear potentiometric titration. The physical characteristics including solubility and viscosity were assessed. The COS was characterized by the degree of polymerization (DP) and FTIR spectroscopy by examining the structural changes before and after the degradation of chitosan. COS showed a higher DDA (99.69 % ± 0.28) compared to chitosan obtained from Sigma Aldrich (77.54 % ±0.28). This enzymatic product, COS also showed high solubility in aqueous and acidic solutions as compared to the chitosan sample. The product of hydrolysis of chitosan mainly composed of COS, with the DP 1-2. The IR spectrum showed that there was no significant change of chitosan before and after the enzymatic hydrolysis confirming the conservation of the chemical phase of the chitosan. In conclusion, COS preparation using cellulase, a low-cost commercial enzyme could open a wide range of new potential applications to improve the quality of many foods. |
| first_indexed | 2025-11-15T13:12:22Z |
| format | Article |
| id | upm-95384 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:12:22Z |
| publishDate | 2021 |
| publisher | Innovative Scientific Information and Services Network |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-953842023-02-16T07:02:06Z http://psasir.upm.edu.my/id/eprint/95384/ The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides Nurhayati, Yusof Mohamad Azman, Ezzat Abd. Ghani, Asmaliza Yusof, Norzaida Tang, John Yew Huat Chitosan is a source of potential bioactive material with many uses in the food, health and agricultural sectors. However, the limitation to utilize the chitosan in biological applications, comprising poor solubility under physiological conditions. In order to overcome the limitations, chitosan is being partially hydrolysed to produce chitosan oligosaccharides (COS). The objective of this research was to determine the degree of deacetylation (DDA), solubility and viscosity of the chitosan and COS. In this study, chitosan was hydrolysed by the cellulase from Trichoderma reesei to produce COS. The DDA of chitosan and COS were determined by linear potentiometric titration. The physical characteristics including solubility and viscosity were assessed. The COS was characterized by the degree of polymerization (DP) and FTIR spectroscopy by examining the structural changes before and after the degradation of chitosan. COS showed a higher DDA (99.69 % ± 0.28) compared to chitosan obtained from Sigma Aldrich (77.54 % ±0.28). This enzymatic product, COS also showed high solubility in aqueous and acidic solutions as compared to the chitosan sample. The product of hydrolysis of chitosan mainly composed of COS, with the DP 1-2. The IR spectrum showed that there was no significant change of chitosan before and after the enzymatic hydrolysis confirming the conservation of the chemical phase of the chitosan. In conclusion, COS preparation using cellulase, a low-cost commercial enzyme could open a wide range of new potential applications to improve the quality of many foods. Innovative Scientific Information and Services Network 2021 Article PeerReviewed Nurhayati, Yusof and Mohamad Azman, Ezzat and Abd. Ghani, Asmaliza and Yusof, Norzaida and Tang, John Yew Huat (2021) The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides. Bioscience Research, 18 (spec.2). 323 - 333. ISSN 1811-9506; ESSN: 2218-3973 https://www.isisn.org/BR-18-SI-2-2021.htm |
| spellingShingle | Nurhayati, Yusof Mohamad Azman, Ezzat Abd. Ghani, Asmaliza Yusof, Norzaida Tang, John Yew Huat The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| title | The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| title_full | The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| title_fullStr | The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| title_full_unstemmed | The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| title_short | The effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| title_sort | effect of cellulase-hydrolyzed chitosan on the degree of deacetylation, solubility and viscosity of chitosan oligosaccharides |
| url | http://psasir.upm.edu.my/id/eprint/95384/ http://psasir.upm.edu.my/id/eprint/95384/ |