Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes
Mungbean (subgenus Ceratotropis) is an important food source in many parts of the world, particularly in Asia and Southeast Asia. It is an important source of plant protein and calcium, and is a good substitute for meat. Although it is an important crop, little is known about its genetic backgrou...
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| Format: | Thesis |
| Language: | English English |
| Published: |
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/9533/ http://psasir.upm.edu.my/id/eprint/9533/1/FSAS_2003_1_A.pdf |
| _version_ | 1848841171872251904 |
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| author | Vijay Kumar, |
| author_facet | Vijay Kumar, |
| author_sort | Vijay Kumar, |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mungbean (subgenus Ceratotropis) is an important food source in many parts of the
world, particularly in Asia and Southeast Asia. It is an important source of plant
protein and calcium, and is a good substitute for meat. Although it is an important
crop, little is known about its genetic background. DNA markers, in particular
micro satellites, are able to provide insights regarding the genetic structure and
background of populations and thus would be of great benefit in mungbean
improvement programs.
Three techniques were used to isolate micro satellite loci in mungbean, namely direct
amplification of length polymorphism (DALP), 5' anchored PCR and random
hybridizing micro satellites (RAHM). A total of 107 repeat sequences were identified
of which 80% were micro satellite loci and 20% were cryptic simple regions. The
majority of micro satellites were found using the 5' anchored PCR procedure which proved to be the most efficient technique in the present study, while DALP did not
produce any microsatellite. |
| first_indexed | 2025-11-15T07:39:00Z |
| format | Thesis |
| id | upm-9533 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:39:00Z |
| publishDate | 2003 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-95332012-08-27T05:17:04Z http://psasir.upm.edu.my/id/eprint/9533/ Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes Vijay Kumar, Mungbean (subgenus Ceratotropis) is an important food source in many parts of the world, particularly in Asia and Southeast Asia. It is an important source of plant protein and calcium, and is a good substitute for meat. Although it is an important crop, little is known about its genetic background. DNA markers, in particular micro satellites, are able to provide insights regarding the genetic structure and background of populations and thus would be of great benefit in mungbean improvement programs. Three techniques were used to isolate micro satellite loci in mungbean, namely direct amplification of length polymorphism (DALP), 5' anchored PCR and random hybridizing micro satellites (RAHM). A total of 107 repeat sequences were identified of which 80% were micro satellite loci and 20% were cryptic simple regions. The majority of micro satellites were found using the 5' anchored PCR procedure which proved to be the most efficient technique in the present study, while DALP did not produce any microsatellite. 2003-01 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/9533/1/FSAS_2003_1_A.pdf Vijay Kumar, (2003) Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes. PhD thesis, Universiti Putra Malaysia. Microsatellites (Genetics). Genetic markers. Mung bean. English |
| spellingShingle | Microsatellites (Genetics). Genetic markers. Mung bean. Vijay Kumar, Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes |
| title | Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes |
| title_full | Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes |
| title_fullStr | Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes |
| title_full_unstemmed | Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes |
| title_short | Isolation, Characterization and Application of DNA Microsatellite Markers in Mungbean (Vigna Radiata L. Wilczek) and Other Selected Legumes |
| title_sort | isolation, characterization and application of dna microsatellite markers in mungbean (vigna radiata l. wilczek) and other selected legumes |
| topic | Microsatellites (Genetics). Genetic markers. Mung bean. |
| url | http://psasir.upm.edu.my/id/eprint/9533/ http://psasir.upm.edu.my/id/eprint/9533/1/FSAS_2003_1_A.pdf |