Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode
The indole alkaloid, mitragynine, is the prominent substance in Mitragyna speciosa Korth. (kratom), the use of which has led to addiction potential and adverse health effects. In this study, an electrochemical immunosensor was developed for the first time for the sensitive and rapid detection of mit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier BV
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95042/ http://psasir.upm.edu.my/id/eprint/95042/1/Sensitive%20detection%20of%20mitragynine%20from%20Mitragyna%20speciosa%20Korth%20using%20an%20electrochemical%20immunosensor%20based%20on%20multiwalled%20carbon%20nanotubes%20chitosan%20%20modified%20carbon%20electrode.pdf |
| _version_ | 1848862059064721408 |
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| author | Mustafa, Radhiahtul Raehan Sukor, Rashidah Eissa, Shimaa Shahrom, Ainaa Najihah Saari, Nazamid Mohd Nor, Siti Mariam |
| author_facet | Mustafa, Radhiahtul Raehan Sukor, Rashidah Eissa, Shimaa Shahrom, Ainaa Najihah Saari, Nazamid Mohd Nor, Siti Mariam |
| author_sort | Mustafa, Radhiahtul Raehan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The indole alkaloid, mitragynine, is the prominent substance in Mitragyna speciosa Korth. (kratom), the use of which has led to addiction potential and adverse health effects. In this study, an electrochemical immunosensor was developed for the first time for the sensitive and rapid detection of mitragynine. Multiwalled carbon nanotubes (MWCNTs)/ chitosan (CS) nanocomposite was utilised as modifier for the sensor fabrication. The detection of mitragynine was based on an indirect competitive assay where, the electrochemical signal was produced from the enzymatic reaction of HRP-modified secondary antibody using 3,3’,5,5’-tetramethylbenzidine (TMB) as substrate. The electrochemical immunosensor exhibited higher sensitivity (10-fold) over conventional ELISA with a limit of detection (LOD) of 0.018 μg/mL and limit of quantification (LOQ) of 0.06 μg/mL. Detection of mitragynine in spiked urine samples resulted in good recoveries between 70–110 %. Hence, the developed immunosensor is a useful tool to detect mitragynine in urine to control the misuse of kratom. |
| first_indexed | 2025-11-15T13:11:00Z |
| format | Article |
| id | upm-95042 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:11:00Z |
| publishDate | 2021 |
| publisher | Elsevier BV |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-950422023-01-05T03:54:12Z http://psasir.upm.edu.my/id/eprint/95042/ Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode Mustafa, Radhiahtul Raehan Sukor, Rashidah Eissa, Shimaa Shahrom, Ainaa Najihah Saari, Nazamid Mohd Nor, Siti Mariam The indole alkaloid, mitragynine, is the prominent substance in Mitragyna speciosa Korth. (kratom), the use of which has led to addiction potential and adverse health effects. In this study, an electrochemical immunosensor was developed for the first time for the sensitive and rapid detection of mitragynine. Multiwalled carbon nanotubes (MWCNTs)/ chitosan (CS) nanocomposite was utilised as modifier for the sensor fabrication. The detection of mitragynine was based on an indirect competitive assay where, the electrochemical signal was produced from the enzymatic reaction of HRP-modified secondary antibody using 3,3’,5,5’-tetramethylbenzidine (TMB) as substrate. The electrochemical immunosensor exhibited higher sensitivity (10-fold) over conventional ELISA with a limit of detection (LOD) of 0.018 μg/mL and limit of quantification (LOQ) of 0.06 μg/mL. Detection of mitragynine in spiked urine samples resulted in good recoveries between 70–110 %. Hence, the developed immunosensor is a useful tool to detect mitragynine in urine to control the misuse of kratom. Elsevier BV 2021-06-24 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/95042/1/Sensitive%20detection%20of%20mitragynine%20from%20Mitragyna%20speciosa%20Korth%20using%20an%20electrochemical%20immunosensor%20based%20on%20multiwalled%20carbon%20nanotubes%20chitosan%20%20modified%20carbon%20electrode.pdf Mustafa, Radhiahtul Raehan and Sukor, Rashidah and Eissa, Shimaa and Shahrom, Ainaa Najihah and Saari, Nazamid and Mohd Nor, Siti Mariam (2021) Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode. Sensors and Actuators B: Chemical, 345. 01-10. ISSN 0925-4005; ESSN: 1873-3077 https://www.sciencedirect.com/science/article/pii/S0925400521009242 10.1016/j.snb.2021.130356 |
| spellingShingle | Mustafa, Radhiahtul Raehan Sukor, Rashidah Eissa, Shimaa Shahrom, Ainaa Najihah Saari, Nazamid Mohd Nor, Siti Mariam Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| title | Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| title_full | Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| title_fullStr | Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| title_full_unstemmed | Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| title_short | Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| title_sort | sensitive detection of mitragynine from mitragyna speciosa korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/chitosan- modified carbon electrode |
| url | http://psasir.upm.edu.my/id/eprint/95042/ http://psasir.upm.edu.my/id/eprint/95042/ http://psasir.upm.edu.my/id/eprint/95042/ http://psasir.upm.edu.my/id/eprint/95042/1/Sensitive%20detection%20of%20mitragynine%20from%20Mitragyna%20speciosa%20Korth%20using%20an%20electrochemical%20immunosensor%20based%20on%20multiwalled%20carbon%20nanotubes%20chitosan%20%20modified%20carbon%20electrode.pdf |