Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells
Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total ph...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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MDPI AG
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/95029/ |
| _version_ | 1848862055986102272 |
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| author | Kumar, Muganti Rajah Swee, Keong Yeap Han, Chun Kwong Mohamad, Nurul Elyani Mubin Aziz, Muhammad Nazirul Khalid, Melati Masarudin, Mas Jaffri Thean, Adam Chor Leow Abdullah, Janna Ong Alitheen, Noorjahan Banu |
| author_facet | Kumar, Muganti Rajah Swee, Keong Yeap Han, Chun Kwong Mohamad, Nurul Elyani Mubin Aziz, Muhammad Nazirul Khalid, Melati Masarudin, Mas Jaffri Thean, Adam Chor Leow Abdullah, Janna Ong Alitheen, Noorjahan Banu |
| author_sort | Kumar, Muganti Rajah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assays, whereas the anti-apoptotic activity on hydrogen peroxide (H2O2) induced SH-SY5Y cells was examined using MTT, AO/PI double staining, and PI/Annexin V-FITC assays. The surface and internal morphological features of SH-SY5Y cells were studied using scanning and transmission electron microscopy. The results indicate that Kefir B showed the higher TPC (1.96 ± 0.54 µg GAE/µL), TFC (1.09 ± 0.02 µg CAT eq/µL), FRAP (19.68 ± 0.11 mM FRAP eq/50 µL), and DPPH (0.45 ± 0.06 mg/mL) activities compared to the other kefir samples. The MTT and PI/Annexin V-FITC assays showed that Kefir B pre-treatment at 10 mg/mL for 48 h resulted in greater cytoprotection (97.04%), and a significantly lower percentage of necrotic cells (7.79%), respectively. The Kefir B pre-treatment also resulted in greater protection to cytoplasmic and cytoskeleton inclusion, along with the conservation of the surface morphological features and the overall integrity of SH-SY5Y cells. Our findings indicate that the anti-oxidative, anti-apoptosis, and neuroprotective effects of kefir were mediated via the upregulation of SOD and catalase, as well as the modulation of apoptotic genes (Tp73, Bax, and Bcl-2). |
| first_indexed | 2025-11-15T13:10:57Z |
| format | Article |
| id | upm-95029 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:10:57Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-950292023-02-02T03:38:03Z http://psasir.upm.edu.my/id/eprint/95029/ Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells Kumar, Muganti Rajah Swee, Keong Yeap Han, Chun Kwong Mohamad, Nurul Elyani Mubin Aziz, Muhammad Nazirul Khalid, Melati Masarudin, Mas Jaffri Thean, Adam Chor Leow Abdullah, Janna Ong Alitheen, Noorjahan Banu Kefir, a fermented probiotic drink was tested for its potential anti-oxidative, anti-apoptotic, and neuroprotective effects to attenuate cellular oxidative stress on human SH-SY5Y neuroblastoma cells. Here, the antioxidant potentials of the six different kefir water samples were analysed by total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH) assays, whereas the anti-apoptotic activity on hydrogen peroxide (H2O2) induced SH-SY5Y cells was examined using MTT, AO/PI double staining, and PI/Annexin V-FITC assays. The surface and internal morphological features of SH-SY5Y cells were studied using scanning and transmission electron microscopy. The results indicate that Kefir B showed the higher TPC (1.96 ± 0.54 µg GAE/µL), TFC (1.09 ± 0.02 µg CAT eq/µL), FRAP (19.68 ± 0.11 mM FRAP eq/50 µL), and DPPH (0.45 ± 0.06 mg/mL) activities compared to the other kefir samples. The MTT and PI/Annexin V-FITC assays showed that Kefir B pre-treatment at 10 mg/mL for 48 h resulted in greater cytoprotection (97.04%), and a significantly lower percentage of necrotic cells (7.79%), respectively. The Kefir B pre-treatment also resulted in greater protection to cytoplasmic and cytoskeleton inclusion, along with the conservation of the surface morphological features and the overall integrity of SH-SY5Y cells. Our findings indicate that the anti-oxidative, anti-apoptosis, and neuroprotective effects of kefir were mediated via the upregulation of SOD and catalase, as well as the modulation of apoptotic genes (Tp73, Bax, and Bcl-2). MDPI AG 2021-06-10 Article PeerReviewed Kumar, Muganti Rajah and Swee, Keong Yeap and Han, Chun Kwong and Mohamad, Nurul Elyani and Mubin Aziz, Muhammad Nazirul and Khalid, Melati and Masarudin, Mas Jaffri and Thean, Adam Chor Leow and Abdullah, Janna Ong and Alitheen, Noorjahan Banu (2021) Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells. Antioxidants, 10 (6). art. no. 940. pp. 1-22. ISSN 2076-3921 https://www.mdpi.com/2076-3921/10/6/940 10.3390/antiox10060940 |
| spellingShingle | Kumar, Muganti Rajah Swee, Keong Yeap Han, Chun Kwong Mohamad, Nurul Elyani Mubin Aziz, Muhammad Nazirul Khalid, Melati Masarudin, Mas Jaffri Thean, Adam Chor Leow Abdullah, Janna Ong Alitheen, Noorjahan Banu Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells |
| title | Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells |
| title_full | Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells |
| title_fullStr | Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells |
| title_full_unstemmed | Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells |
| title_short | Selected Kefir Water from Malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in SH-SY5Y neuroblastoma cells |
| title_sort | selected kefir water from malaysia attenuates hydrogen per-oxide-induced oxidative stress by upregulating endogenous antioxidant levels in sh-sy5y neuroblastoma cells |
| url | http://psasir.upm.edu.my/id/eprint/95029/ http://psasir.upm.edu.my/id/eprint/95029/ http://psasir.upm.edu.my/id/eprint/95029/ |