Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe
The phylogenetic relationships among species of the genus Eurema from Peninsular Malaysia were reconstructed using nucleotide sequences of mitochondrial CO1 (307 bp) and nuclear ribosomal 28S DNA (471 bp). A total of twenty-eight sequences generated through PCR amplification for each gene region w...
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| Format: | Article |
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Raffles Museum of Biodiversity Research
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94576/ |
| _version_ | 1848862035571376128 |
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| author | Abdul Wahid, Noor Azrizal Idid, Mohammed Rizman Azirun, Mohd Sofian |
| author_facet | Abdul Wahid, Noor Azrizal Idid, Mohammed Rizman Azirun, Mohd Sofian |
| author_sort | Abdul Wahid, Noor Azrizal |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The phylogenetic relationships among species of the genus Eurema from Peninsular Malaysia were
reconstructed using nucleotide sequences of mitochondrial CO1 (307 bp) and nuclear ribosomal 28S DNA (471 bp).
A total of twenty-eight sequences generated through PCR amplification for each gene region were used to construct
the Maximum Likelihood (ML) and Maximum Parsimony (MP) phylogenetic trees. The separate taxonomic grouping
of the genus Eurema and the genus Gandaca, and their close association is tested here together with sequences of
other pierid butterflies obtained from GenBank. All trees reveal a strongly supported monophyletic group of Eurema
conspecifics and well-resolved interspecific genetic distances, indicating the usefulness of the genetic markers in
local species identification. The combined phylogenetic analyses of CO1 and 28S genes strongly supports a close
relationship of E. hecabe with E. blanda, while E. andersonii is recovered as a sister taxon to E. ada. |
| first_indexed | 2025-11-15T13:10:37Z |
| format | Article |
| id | upm-94576 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:10:37Z |
| publishDate | 2021 |
| publisher | Raffles Museum of Biodiversity Research |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-945762022-12-05T06:21:18Z http://psasir.upm.edu.my/id/eprint/94576/ Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe Abdul Wahid, Noor Azrizal Idid, Mohammed Rizman Azirun, Mohd Sofian The phylogenetic relationships among species of the genus Eurema from Peninsular Malaysia were reconstructed using nucleotide sequences of mitochondrial CO1 (307 bp) and nuclear ribosomal 28S DNA (471 bp). A total of twenty-eight sequences generated through PCR amplification for each gene region were used to construct the Maximum Likelihood (ML) and Maximum Parsimony (MP) phylogenetic trees. The separate taxonomic grouping of the genus Eurema and the genus Gandaca, and their close association is tested here together with sequences of other pierid butterflies obtained from GenBank. All trees reveal a strongly supported monophyletic group of Eurema conspecifics and well-resolved interspecific genetic distances, indicating the usefulness of the genetic markers in local species identification. The combined phylogenetic analyses of CO1 and 28S genes strongly supports a close relationship of E. hecabe with E. blanda, while E. andersonii is recovered as a sister taxon to E. ada. Raffles Museum of Biodiversity Research 2021-07 Article PeerReviewed Abdul Wahid, Noor Azrizal and Idid, Mohammed Rizman and Azirun, Mohd Sofian (2021) Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe. Raffles Bulletin of Zoology, 69. 262 - 271. ISSN 0217-2445 https://lkcnhm.nus.edu.sg/publications/raffles-bulletin-of-zoology/volumes/volume-69/ 10.26107/RBZ-2021-0021 |
| spellingShingle | Abdul Wahid, Noor Azrizal Idid, Mohammed Rizman Azirun, Mohd Sofian Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe |
| title | Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe |
| title_full | Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe |
| title_fullStr | Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe |
| title_full_unstemmed | Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe |
| title_short | Phylogenetic relationships of Eurema butterflies from Peninsular Malaysia inferred from COI and 28S gene sequences with emphasis on Eurema hecabe |
| title_sort | phylogenetic relationships of eurema butterflies from peninsular malaysia inferred from coi and 28s gene sequences with emphasis on eurema hecabe |
| url | http://psasir.upm.edu.my/id/eprint/94576/ http://psasir.upm.edu.my/id/eprint/94576/ http://psasir.upm.edu.my/id/eprint/94576/ |