| Summary: | The phylogenetic relationships among species of the genus Eurema from Peninsular Malaysia were
reconstructed using nucleotide sequences of mitochondrial CO1 (307 bp) and nuclear ribosomal 28S DNA (471 bp).
A total of twenty-eight sequences generated through PCR amplification for each gene region were used to construct
the Maximum Likelihood (ML) and Maximum Parsimony (MP) phylogenetic trees. The separate taxonomic grouping
of the genus Eurema and the genus Gandaca, and their close association is tested here together with sequences of
other pierid butterflies obtained from GenBank. All trees reveal a strongly supported monophyletic group of Eurema
conspecifics and well-resolved interspecific genetic distances, indicating the usefulness of the genetic markers in
local species identification. The combined phylogenetic analyses of CO1 and 28S genes strongly supports a close
relationship of E. hecabe with E. blanda, while E. andersonii is recovered as a sister taxon to E. ada.
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