Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl.
Barleria lupulina Lindl. (Acanthaceae) as an ornamental plant has been widely used in folklore medicine due to its abundancy in polyphenolic compounds. The present study examined conditions for optimal extraction of antioxidants from B. lupulina leaf extracts by using the microwave-assisted extrac...
| Main Authors: | , , , , |
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| Format: | Article |
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MDPI
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94468/ |
| _version_ | 1848862006107439104 |
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| author | Ismail Suhaimy, Noor Wahida Abd Gani, Siti Salwa Zaidan, Uswatun Hasanah Halmi, Mohd Izuan Effendi Bawon, Paiman |
| author_facet | Ismail Suhaimy, Noor Wahida Abd Gani, Siti Salwa Zaidan, Uswatun Hasanah Halmi, Mohd Izuan Effendi Bawon, Paiman |
| author_sort | Ismail Suhaimy, Noor Wahida |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Barleria lupulina Lindl. (Acanthaceae) as an ornamental plant has been widely used in
folklore medicine due to its abundancy in polyphenolic compounds. The present study examined conditions for optimal extraction of antioxidants from B. lupulina leaf extracts by using the
microwave-assisted extraction (MAE) method. The effects of ethanol concentrations, microwave
power, and extraction time on total phenolic content (TPC), total flavonoid content (TFC), 1-diphenyl2-picrylhydrazyl (DPPH), and 2,20-azino-bis (3-ethylbenzothizoline-6-sulfonic acid) (ABTS) were
investigated by single-factor experiments. Response surface methodology (RSM) was applied to
observe interactions of three independent variables (ethanol concentrations, microwave power, and
extraction time) on the dependent variables (TPC, TFC, DPPH, and ABTS) to establish optimal extraction conditions. Quadratic polynomial equations in all experimental models yielded favorably with
fitted models with R2 and R2
adj of more than 0.90 and a non-significant lack of fit at p > 0.05. The optimal conditions for the extraction of antioxidant activity were established at 80% (v/v) ethanol, 400 W,
and 30 s with TPC (238.71 mg gallic acid equivalent (GAE)/g sample), TFC (58.09 mg QE/g sample),
DPPH (87.95%), and ABTS (89.56%). Analysis by ultra-high-performance liquid chromatography–
quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) successfully identified four new
phenylethanoid glycoside compounds in the species. |
| first_indexed | 2025-11-15T13:10:09Z |
| format | Article |
| id | upm-94468 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:10:09Z |
| publishDate | 2021 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-944682023-02-07T03:54:18Z http://psasir.upm.edu.my/id/eprint/94468/ Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. Ismail Suhaimy, Noor Wahida Abd Gani, Siti Salwa Zaidan, Uswatun Hasanah Halmi, Mohd Izuan Effendi Bawon, Paiman Barleria lupulina Lindl. (Acanthaceae) as an ornamental plant has been widely used in folklore medicine due to its abundancy in polyphenolic compounds. The present study examined conditions for optimal extraction of antioxidants from B. lupulina leaf extracts by using the microwave-assisted extraction (MAE) method. The effects of ethanol concentrations, microwave power, and extraction time on total phenolic content (TPC), total flavonoid content (TFC), 1-diphenyl2-picrylhydrazyl (DPPH), and 2,20-azino-bis (3-ethylbenzothizoline-6-sulfonic acid) (ABTS) were investigated by single-factor experiments. Response surface methodology (RSM) was applied to observe interactions of three independent variables (ethanol concentrations, microwave power, and extraction time) on the dependent variables (TPC, TFC, DPPH, and ABTS) to establish optimal extraction conditions. Quadratic polynomial equations in all experimental models yielded favorably with fitted models with R2 and R2 adj of more than 0.90 and a non-significant lack of fit at p > 0.05. The optimal conditions for the extraction of antioxidant activity were established at 80% (v/v) ethanol, 400 W, and 30 s with TPC (238.71 mg gallic acid equivalent (GAE)/g sample), TFC (58.09 mg QE/g sample), DPPH (87.95%), and ABTS (89.56%). Analysis by ultra-high-performance liquid chromatography– quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) successfully identified four new phenylethanoid glycoside compounds in the species. MDPI 2021-04-01 Article PeerReviewed Ismail Suhaimy, Noor Wahida and Abd Gani, Siti Salwa and Zaidan, Uswatun Hasanah and Halmi, Mohd Izuan Effendi and Bawon, Paiman (2021) Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. Plants-Basel, 10 (4). art. no. 682. pp. 1-19. ISSN 2223-7747 https://www.mdpi.com/2223-7747/10/4/682 10.3390/plants10040682 |
| spellingShingle | Ismail Suhaimy, Noor Wahida Abd Gani, Siti Salwa Zaidan, Uswatun Hasanah Halmi, Mohd Izuan Effendi Bawon, Paiman Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. |
| title | Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. |
| title_full | Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. |
| title_fullStr | Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. |
| title_full_unstemmed | Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. |
| title_short | Optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of Barleria lupulina Lindl. |
| title_sort | optimizing conditions for microwave-assisted extraction of polyphenolic content and antioxidant activity of barleria lupulina lindl. |
| url | http://psasir.upm.edu.my/id/eprint/94468/ http://psasir.upm.edu.my/id/eprint/94468/ http://psasir.upm.edu.my/id/eprint/94468/ |