Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia
Aims: Knowledge of the Trichoderma taxa is important for both control efficiency and environmental conservation. Therefore, the objective of this study is to isolate and identify Trichoderma species from various rhizosphere soil samples using phenotypic and molecular characterization. Methodology a...
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| Format: | Article |
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Malaysian Society for Microbiology
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94267/ |
| _version_ | 1848861951489212416 |
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| author | Syd Abdul Rahman, Sharifah Siti Maryam Mohd Zainudin, Nur Ain Izzati Aris, Asma Abd Aziz, Nor Azwady |
| author_facet | Syd Abdul Rahman, Sharifah Siti Maryam Mohd Zainudin, Nur Ain Izzati Aris, Asma Abd Aziz, Nor Azwady |
| author_sort | Syd Abdul Rahman, Sharifah Siti Maryam |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Aims: Knowledge of the Trichoderma taxa is important for both control efficiency and environmental conservation. Therefore, the objective of this study is to isolate and identify Trichoderma species from various rhizosphere soil samples using phenotypic and molecular characterization.
Methodology and results: Native Trichoderma spp. were isolated from agricultural fields in 17 sites from seven states of Malaysia. These isolates were characterized via morphological observation and molecular phylogenetic analysis based on the translation elongation factor-1α (tef1-α) gene. About 42 isolates were classified into eight Trichoderma species, which are Trichoderma asperellum, T. hamatum, T. harzianum, T. koningiopsis, T. rodmanii, T. spirale, T. viride and T. virens. Comparison of DNA sequences of tef1-α showed that the isolates were 98-100% similar to respective Trichoderma species from GenBank, thus confirming the fungal identity. Phylogenetic trees of maximum likelihood (ML) dataset of tef1-α inferred that the isolates were clustered according to species.
Conclusion, significance and impact of study: Findings in the present study will be beneficial for the purposes of
biodiversity conservation and plant disease management using biocontrol agents. |
| first_indexed | 2025-11-15T13:09:17Z |
| format | Article |
| id | upm-94267 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:09:17Z |
| publishDate | 2021 |
| publisher | Malaysian Society for Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-942672023-05-08T04:23:25Z http://psasir.upm.edu.my/id/eprint/94267/ Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia Syd Abdul Rahman, Sharifah Siti Maryam Mohd Zainudin, Nur Ain Izzati Aris, Asma Abd Aziz, Nor Azwady Aims: Knowledge of the Trichoderma taxa is important for both control efficiency and environmental conservation. Therefore, the objective of this study is to isolate and identify Trichoderma species from various rhizosphere soil samples using phenotypic and molecular characterization. Methodology and results: Native Trichoderma spp. were isolated from agricultural fields in 17 sites from seven states of Malaysia. These isolates were characterized via morphological observation and molecular phylogenetic analysis based on the translation elongation factor-1α (tef1-α) gene. About 42 isolates were classified into eight Trichoderma species, which are Trichoderma asperellum, T. hamatum, T. harzianum, T. koningiopsis, T. rodmanii, T. spirale, T. viride and T. virens. Comparison of DNA sequences of tef1-α showed that the isolates were 98-100% similar to respective Trichoderma species from GenBank, thus confirming the fungal identity. Phylogenetic trees of maximum likelihood (ML) dataset of tef1-α inferred that the isolates were clustered according to species. Conclusion, significance and impact of study: Findings in the present study will be beneficial for the purposes of biodiversity conservation and plant disease management using biocontrol agents. Malaysian Society for Microbiology 2021-02 Article PeerReviewed Syd Abdul Rahman, Sharifah Siti Maryam and Mohd Zainudin, Nur Ain Izzati and Aris, Asma and Abd Aziz, Nor Azwady (2021) Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia. Malaysian Journal of Microbiology, 17 (1). 80 - 89. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=2558&slug=Morphological-and-molecular-characterization-of-Trichoderma-species-isolated-from-rhizosphere-soils-in-Malaysia 10.21161/mjm.200893 |
| spellingShingle | Syd Abdul Rahman, Sharifah Siti Maryam Mohd Zainudin, Nur Ain Izzati Aris, Asma Abd Aziz, Nor Azwady Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia |
| title | Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia |
| title_full | Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia |
| title_fullStr | Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia |
| title_full_unstemmed | Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia |
| title_short | Morphological and molecular characterization of Trichoderma species isolated from rhizosphere soils in Malaysia |
| title_sort | morphological and molecular characterization of trichoderma species isolated from rhizosphere soils in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/94267/ http://psasir.upm.edu.my/id/eprint/94267/ http://psasir.upm.edu.my/id/eprint/94267/ |