Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken
Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH. Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity...
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| Format: | Article |
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Korean Society of Veterinary Science
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94246/ |
| _version_ | 1848861946150912000 |
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| author | Sabarudin, Nur Syazana Tan, Sheau Wei Phang, Yuen Fun Omar, Abdul Rahman |
| author_facet | Sabarudin, Nur Syazana Tan, Sheau Wei Phang, Yuen Fun Omar, Abdul Rahman |
| author_sort | Sabarudin, Nur Syazana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH.
Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity of FAdV-8b in specific-pathogen-free (SPF) chickens following virus inoculation via oral and intramuscular (IM) routes.
Methods: Suspected organ samples were subjected to virus isolation and polymerase chain reaction (PCR) for FAdV detection. Hexon gene sequencing and phylogenetic analysis were performed on FAdV-positive samples for serotype identification. One FAdV-8b isolate, UPM/FAdV/420/2017, was selected for fiber gene characterization and pathogenicity study and was inoculated in SPF chickens via oral and IM routes.
Results: The hexon gene phylogenetic analysis revealed that all isolates belonged to FAdV8b. The fiber gene-based phylogenetic analysis of isolate UPM/FAdV/420/2017 supported the grouping of that isolate into FAdV species E. Pathogenicity study revealed that, chickens infected with UPM/FAdV/420/2017 via the IM route had higher clinical score values, higher percent mortality, higher degree of the liver lesions, higher antibody response (p < 0.05), and higher virus shedding amounts (p < 0.05) than those infected via the oral route. The highest virus copy numbers were detected in liver and gizzard.
Conclusions: FAdV-8b is the dominant FAdV serotype in Malaysia, and pathogenicity study of the FAdV-8b isolate UPM/FAdV/420/2017 indicated its ability to induce IBH in young SPF chickens when infected via oral or IM routes. |
| first_indexed | 2025-11-15T13:09:12Z |
| format | Article |
| id | upm-94246 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:09:12Z |
| publishDate | 2021 |
| publisher | Korean Society of Veterinary Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-942462023-05-09T01:58:58Z http://psasir.upm.edu.my/id/eprint/94246/ Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken Sabarudin, Nur Syazana Tan, Sheau Wei Phang, Yuen Fun Omar, Abdul Rahman Background: Inclusion body hepatitis (IBH) is an economically important viral disease primarily affecting broiler and breeder chickens. All 12 serotypes of fowl adenovirus (FAdV) can cause IBH. Objectives: To characterize FAdV isolates based on phylogenetic analysis, and to study the pathogenicity of FAdV-8b in specific-pathogen-free (SPF) chickens following virus inoculation via oral and intramuscular (IM) routes. Methods: Suspected organ samples were subjected to virus isolation and polymerase chain reaction (PCR) for FAdV detection. Hexon gene sequencing and phylogenetic analysis were performed on FAdV-positive samples for serotype identification. One FAdV-8b isolate, UPM/FAdV/420/2017, was selected for fiber gene characterization and pathogenicity study and was inoculated in SPF chickens via oral and IM routes. Results: The hexon gene phylogenetic analysis revealed that all isolates belonged to FAdV8b. The fiber gene-based phylogenetic analysis of isolate UPM/FAdV/420/2017 supported the grouping of that isolate into FAdV species E. Pathogenicity study revealed that, chickens infected with UPM/FAdV/420/2017 via the IM route had higher clinical score values, higher percent mortality, higher degree of the liver lesions, higher antibody response (p < 0.05), and higher virus shedding amounts (p < 0.05) than those infected via the oral route. The highest virus copy numbers were detected in liver and gizzard. Conclusions: FAdV-8b is the dominant FAdV serotype in Malaysia, and pathogenicity study of the FAdV-8b isolate UPM/FAdV/420/2017 indicated its ability to induce IBH in young SPF chickens when infected via oral or IM routes. Korean Society of Veterinary Science 2021-05-11 Article PeerReviewed Sabarudin, Nur Syazana and Tan, Sheau Wei and Phang, Yuen Fun and Omar, Abdul Rahman (2021) Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken. Journal of Veterinary Science, 22 (4). art. no. e42. pp. 1-16. ISSN 1229-845X; ESSN 1976-555X https://www.vetsci.org/search.php?where=aview&id=10.4142/jvs.2021.22.e42&code=0118JVS&vmode=FULL 10.4142/jvs.2021.22.e42 |
| spellingShingle | Sabarudin, Nur Syazana Tan, Sheau Wei Phang, Yuen Fun Omar, Abdul Rahman Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken |
| title | Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken |
| title_full | Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken |
| title_fullStr | Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken |
| title_full_unstemmed | Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken |
| title_short | Molecular characterization of Malaysian fowl adenovirus (FAdV) serotype 8b species E and pathogenicity of the virus in specific pathogen-free chicken |
| title_sort | molecular characterization of malaysian fowl adenovirus (fadv) serotype 8b species e and pathogenicity of the virus in specific pathogen-free chicken |
| url | http://psasir.upm.edu.my/id/eprint/94246/ http://psasir.upm.edu.my/id/eprint/94246/ http://psasir.upm.edu.my/id/eprint/94246/ |