Metabolomic and antioxidant properties of different varieties and origins of dragon fruit
Dragon fruit has appealed much concern from consumers as a novelty fruit with potent nutritional and medicinal benefits. Dragon fruit quality warrants comprehensive evaluation, based on the contents of pigments and health-promoting natural compounds in different varieties. This study was aimed to ev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Elsevier
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94160/ |
| _version_ | 1848861923311878144 |
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| author | Al-Mekhlafi, Nabil Ali Mediani, Ahmed Ismail, Nor Hadiani Abas, Faridah Dymerski, Tomasz Lubinska-Szczygeł, Martyna Vearasilp, Suchada Gorinstein, Shela |
| author_facet | Al-Mekhlafi, Nabil Ali Mediani, Ahmed Ismail, Nor Hadiani Abas, Faridah Dymerski, Tomasz Lubinska-Szczygeł, Martyna Vearasilp, Suchada Gorinstein, Shela |
| author_sort | Al-Mekhlafi, Nabil Ali |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dragon fruit has appealed much concern from consumers as a novelty fruit with potent nutritional and medicinal benefits. Dragon fruit quality warrants comprehensive evaluation, based on the contents of pigments and health-promoting natural compounds in different varieties. This study was aimed to evaluate the differences among dragon fruit varieties extracted with methanol–water (CD3OD-D2O) and methanol (CD3OD) by proton nuclear magnetic resonance (1H NMR)-based metabolomics approach. The variation features of the metabolite profiles were studied between varieties and origins of dragon fruit, considering the differences in principal component analysis (PCA). The hierarchical clustering analysis (HCA) based on score values of PCA model was also performed to analyze the distance between samples based on metabolites contents. The results of 1H NMR spectra showed that the CD3OD-D2O extracts quantitatively differ from CD3OD ones. In dragon fruit extracts, 36 metabolites were identified. The results demonstrated that the methanol and methanol/water extracted similar compounds with higher intensity in methanol. The metabolic differences among varieties were also shown for CD3OD extracts by comparing both Pareto and UV scaling methods. The big size red fleshed dragon fruit (samples 2 and 3), growing in Israel were clustered similar to that growing in Thailand with the abundance of phenolic compounds. Glucose and fructose were more prominent in the yellow and white fleshed fruit (samples 4 and 5) growing in Israel. To support the obtained results two dimensional 1H–1H J-resolved and UHPLC-MS measurements were carried out. This research gain novel insights into the field as the first NMR metabolites fingerprinting of the major dragon fruit varieties. The correlations between DPPH, CUPRAC, antioxidant and metabolomic properties were also evaluated. The chemical markers associated with varieties of dragon fruit quality and their appearances were identified and can be utilized for the basis of authentication purpose of this fruit. |
| first_indexed | 2025-11-15T13:08:50Z |
| format | Article |
| id | upm-94160 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:08:50Z |
| publishDate | 2021 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-941602023-03-28T06:32:16Z http://psasir.upm.edu.my/id/eprint/94160/ Metabolomic and antioxidant properties of different varieties and origins of dragon fruit Al-Mekhlafi, Nabil Ali Mediani, Ahmed Ismail, Nor Hadiani Abas, Faridah Dymerski, Tomasz Lubinska-Szczygeł, Martyna Vearasilp, Suchada Gorinstein, Shela Dragon fruit has appealed much concern from consumers as a novelty fruit with potent nutritional and medicinal benefits. Dragon fruit quality warrants comprehensive evaluation, based on the contents of pigments and health-promoting natural compounds in different varieties. This study was aimed to evaluate the differences among dragon fruit varieties extracted with methanol–water (CD3OD-D2O) and methanol (CD3OD) by proton nuclear magnetic resonance (1H NMR)-based metabolomics approach. The variation features of the metabolite profiles were studied between varieties and origins of dragon fruit, considering the differences in principal component analysis (PCA). The hierarchical clustering analysis (HCA) based on score values of PCA model was also performed to analyze the distance between samples based on metabolites contents. The results of 1H NMR spectra showed that the CD3OD-D2O extracts quantitatively differ from CD3OD ones. In dragon fruit extracts, 36 metabolites were identified. The results demonstrated that the methanol and methanol/water extracted similar compounds with higher intensity in methanol. The metabolic differences among varieties were also shown for CD3OD extracts by comparing both Pareto and UV scaling methods. The big size red fleshed dragon fruit (samples 2 and 3), growing in Israel were clustered similar to that growing in Thailand with the abundance of phenolic compounds. Glucose and fructose were more prominent in the yellow and white fleshed fruit (samples 4 and 5) growing in Israel. To support the obtained results two dimensional 1H–1H J-resolved and UHPLC-MS measurements were carried out. This research gain novel insights into the field as the first NMR metabolites fingerprinting of the major dragon fruit varieties. The correlations between DPPH, CUPRAC, antioxidant and metabolomic properties were also evaluated. The chemical markers associated with varieties of dragon fruit quality and their appearances were identified and can be utilized for the basis of authentication purpose of this fruit. Elsevier 2021 Article PeerReviewed Al-Mekhlafi, Nabil Ali and Mediani, Ahmed and Ismail, Nor Hadiani and Abas, Faridah and Dymerski, Tomasz and Lubinska-Szczygeł, Martyna and Vearasilp, Suchada and Gorinstein, Shela (2021) Metabolomic and antioxidant properties of different varieties and origins of dragon fruit. Microchemical Journal, 160 (pt. B). pp. 1-12. ISSN 0026-265X; ESSN: 1095-9149 https://www.sciencedirect.com/science/article/pii/S0026265X20322967 10.1016/j.microc.2020.105687 |
| spellingShingle | Al-Mekhlafi, Nabil Ali Mediani, Ahmed Ismail, Nor Hadiani Abas, Faridah Dymerski, Tomasz Lubinska-Szczygeł, Martyna Vearasilp, Suchada Gorinstein, Shela Metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| title | Metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| title_full | Metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| title_fullStr | Metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| title_full_unstemmed | Metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| title_short | Metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| title_sort | metabolomic and antioxidant properties of different varieties and origins of dragon fruit |
| url | http://psasir.upm.edu.my/id/eprint/94160/ http://psasir.upm.edu.my/id/eprint/94160/ http://psasir.upm.edu.my/id/eprint/94160/ |