Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis
Osteoblasts play an important role in bone regeneration and repair. The hypoxia condition in bone occurs when bone undergoes fracture, and this will trigger a series of biochemical and mechanical changes to enable bone repair. Hence, it is interesting to observe the metabolites and metabolism change...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
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Sage Publications
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94155/ |
| _version_ | 1848861921899446272 |
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| author | Cui, Yan Chao Qiu, Yu Sheng Wu, Qiong Bu, Gang Peli, Amira Teh, Seoh Wei Ang, Kok Pian Ms Joseph, Narcisse Koh, Avin Ee-Hwan Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Samrot, Antony V. Mok, Pooi Ling Subbiah, Suresh Kumar |
| author_facet | Cui, Yan Chao Qiu, Yu Sheng Wu, Qiong Bu, Gang Peli, Amira Teh, Seoh Wei Ang, Kok Pian Ms Joseph, Narcisse Koh, Avin Ee-Hwan Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Samrot, Antony V. Mok, Pooi Ling Subbiah, Suresh Kumar |
| author_sort | Cui, Yan Chao |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Osteoblasts play an important role in bone regeneration and repair. The hypoxia condition in bone occurs when bone undergoes fracture, and this will trigger a series of biochemical and mechanical changes to enable bone repair. Hence, it is interesting to observe the metabolites and metabolism changes when osteoblasts are exposed to hypoxic condition. This study has looked into the response of human osteoblast hFOB 1.19 under normoxic and hypoxic conditions by observing the cell growth and utilization of metabolites via Phenotype MicroArrays™ under these two different oxygen concentrations. The cell growth of hFOB 1.19 under hypoxic condition showed better growth compared to hFOB 1.19 under normal condition. In this study, osteoblast used glycolysis as the main pathway to produce energy as hFOB 1.19 in both hypoxic and normoxic conditions showed cell growth in well containing dextrin, glycogen, maltotriose, D-maltose, D-glucose-6-phospate, D-glucose, D-mannose, D-Turanose, D-fructose-6-phosphate, D-galactose, uridine, adenosine, inosine and α-keto-glutaric acid. In hypoxia, the cells have utilized additional metabolites such as α-D-glucose-1-phosphate and D-fructose, indicating possible activation of glycogen synthesis and glycogenolysis to metabolize α-D-glucose-1-phosphate. Meanwhile, during normoxia, D-L-α-glycerol phosphate was used, and this implies that the osteoblast may use glycerol-3-phosphate shuttle and oxidative phosphorylation to metabolize glycerol-3-phosphate. |
| first_indexed | 2025-11-15T13:08:49Z |
| format | Article |
| id | upm-94155 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:08:49Z |
| publishDate | 2021 |
| publisher | Sage Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-941552023-03-28T02:13:36Z http://psasir.upm.edu.my/id/eprint/94155/ Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis Cui, Yan Chao Qiu, Yu Sheng Wu, Qiong Bu, Gang Peli, Amira Teh, Seoh Wei Ang, Kok Pian Ms Joseph, Narcisse Koh, Avin Ee-Hwan Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Samrot, Antony V. Mok, Pooi Ling Subbiah, Suresh Kumar Osteoblasts play an important role in bone regeneration and repair. The hypoxia condition in bone occurs when bone undergoes fracture, and this will trigger a series of biochemical and mechanical changes to enable bone repair. Hence, it is interesting to observe the metabolites and metabolism changes when osteoblasts are exposed to hypoxic condition. This study has looked into the response of human osteoblast hFOB 1.19 under normoxic and hypoxic conditions by observing the cell growth and utilization of metabolites via Phenotype MicroArrays™ under these two different oxygen concentrations. The cell growth of hFOB 1.19 under hypoxic condition showed better growth compared to hFOB 1.19 under normal condition. In this study, osteoblast used glycolysis as the main pathway to produce energy as hFOB 1.19 in both hypoxic and normoxic conditions showed cell growth in well containing dextrin, glycogen, maltotriose, D-maltose, D-glucose-6-phospate, D-glucose, D-mannose, D-Turanose, D-fructose-6-phosphate, D-galactose, uridine, adenosine, inosine and α-keto-glutaric acid. In hypoxia, the cells have utilized additional metabolites such as α-D-glucose-1-phosphate and D-fructose, indicating possible activation of glycogen synthesis and glycogenolysis to metabolize α-D-glucose-1-phosphate. Meanwhile, during normoxia, D-L-α-glycerol phosphate was used, and this implies that the osteoblast may use glycerol-3-phosphate shuttle and oxidative phosphorylation to metabolize glycerol-3-phosphate. Sage Publications 2021-05 Article PeerReviewed Cui, Yan Chao and Qiu, Yu Sheng and Wu, Qiong and Bu, Gang and Peli, Amira and Teh, Seoh Wei and Ang, Kok Pian and Ms Joseph, Narcisse and Koh, Avin Ee-Hwan and Farhana, Aisha and Alzahrani, Badr and Khan, Mohammed Safwan Ali and Samrot, Antony V. and Mok, Pooi Ling and Subbiah, Suresh Kumar (2021) Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis. Experimental Biology and Medicine, 246 (10). 1177 - 1183. ISSN 1535-3702; ESSN: 1535-3699 https://journals.sagepub.com/doi/10.1177/1535370220985468?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed 10.1177/1535370220985468 |
| spellingShingle | Cui, Yan Chao Qiu, Yu Sheng Wu, Qiong Bu, Gang Peli, Amira Teh, Seoh Wei Ang, Kok Pian Ms Joseph, Narcisse Koh, Avin Ee-Hwan Farhana, Aisha Alzahrani, Badr Khan, Mohammed Safwan Ali Samrot, Antony V. Mok, Pooi Ling Subbiah, Suresh Kumar Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| title | Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| title_full | Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| title_fullStr | Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| title_full_unstemmed | Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| title_short | Metabolic utilization of human osteoblast cell line hFOB 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| title_sort | metabolic utilization of human osteoblast cell line hfob 1.19 under normoxic and hypoxic conditions: a phenotypic microarray analysis |
| url | http://psasir.upm.edu.my/id/eprint/94155/ http://psasir.upm.edu.my/id/eprint/94155/ http://psasir.upm.edu.my/id/eprint/94155/ |