Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research
Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic a...
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| Format: | Article |
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MDPI AG
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/94052/ |
| _version_ | 1848861904543416320 |
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| author | Padzil, Faiz Abdul Razak, Mariatulqabtiah Tan, Wen Siang Ho, Kok Lian Mat Isa, Nurulfiza Lau, Han Yih Abu, Jalila Chuang, Kuo-Pin |
| author_facet | Padzil, Faiz Abdul Razak, Mariatulqabtiah Tan, Wen Siang Ho, Kok Lian Mat Isa, Nurulfiza Lau, Han Yih Abu, Jalila Chuang, Kuo-Pin |
| author_sort | Padzil, Faiz |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), quantitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged as a reliable tool to improvise on-field diagnosis without dependence on thermocyclers. Among the established isothermal amplification technologies are loop-mediated isothermal amplification (LAMP), recombinant polymerase amplification (RPA), strand displacement activity (SDA), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA) and rolling circle amplification (RCA). This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses. |
| first_indexed | 2025-11-15T13:08:32Z |
| format | Article |
| id | upm-94052 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:08:32Z |
| publishDate | 2021 |
| publisher | MDPI AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-940522023-05-18T02:24:50Z http://psasir.upm.edu.my/id/eprint/94052/ Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research Padzil, Faiz Abdul Razak, Mariatulqabtiah Tan, Wen Siang Ho, Kok Lian Mat Isa, Nurulfiza Lau, Han Yih Abu, Jalila Chuang, Kuo-Pin Over the years, development of molecular diagnostics has evolved significantly in the detection of pathogens within humans and their surroundings. Researchers have discovered new species and strains of viruses, while mitigating the viral infections that occur, owing to the accessibility of nucleic acid screening methods such as polymerase chain reaction (PCR), quantitative (real-time) polymerase chain reaction (qPCR) and reverse-transcription qPCR (RT-qPCR). While such molecular detection methods are widely utilized as the benchmark, the invention of isothermal amplifications has also emerged as a reliable tool to improvise on-field diagnosis without dependence on thermocyclers. Among the established isothermal amplification technologies are loop-mediated isothermal amplification (LAMP), recombinant polymerase amplification (RPA), strand displacement activity (SDA), nucleic acid sequence-based amplification (NASBA), helicase-dependent amplification (HDA) and rolling circle amplification (RCA). This review highlights the past research on and future prospects of LAMP, its principles and applications as a promising point-of-care diagnostic method against avian viruses. MDPI AG 2021-12-30 Article PeerReviewed Padzil, Faiz and Abdul Razak, Mariatulqabtiah and Tan, Wen Siang and Ho, Kok Lian and Mat Isa, Nurulfiza and Lau, Han Yih and Abu, Jalila and Chuang, Kuo-Pin (2021) Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research. Animals, 12 (1). art. no. 76. pp. 1-19. ISSN 2076-2615 https://www.mdpi.com/2076-2615/12/1/76 10.3390/ani12010076 |
| spellingShingle | Padzil, Faiz Abdul Razak, Mariatulqabtiah Tan, Wen Siang Ho, Kok Lian Mat Isa, Nurulfiza Lau, Han Yih Abu, Jalila Chuang, Kuo-Pin Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research |
| title | Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research |
| title_full | Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research |
| title_fullStr | Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research |
| title_full_unstemmed | Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research |
| title_short | Loop-mediated isothermal amplification (LAMP) as a promising point-of-care diagnostic strategy in avian virus research |
| title_sort | loop-mediated isothermal amplification (lamp) as a promising point-of-care diagnostic strategy in avian virus research |
| url | http://psasir.upm.edu.my/id/eprint/94052/ http://psasir.upm.edu.my/id/eprint/94052/ http://psasir.upm.edu.my/id/eprint/94052/ |