Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo
Klebsiella pneumoniae are opportunistic bacteria found in the gut. In recent years they have been associated with nosocomial infections. The increased incidence of multiple drug-resistant K. pneumoniae makes it necessary to find new alternatives to treat the disease. In this study, phage UPM2146 was...
| Main Authors: | , , , , , |
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| Format: | Article |
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Public Library of Science
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/93983/ |
| _version_ | 1848861890064678912 |
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| author | Assafiri, Omar Song, Adelene Ai Lian Tan, Geok Hun Hanish, Irwan Mohd Hashim, Amalia Yusoff, Khatijah |
| author_facet | Assafiri, Omar Song, Adelene Ai Lian Tan, Geok Hun Hanish, Irwan Mohd Hashim, Amalia Yusoff, Khatijah |
| author_sort | Assafiri, Omar |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Klebsiella pneumoniae are opportunistic bacteria found in the gut. In recent years they have been associated with nosocomial infections. The increased incidence of multiple drug-resistant K. pneumoniae makes it necessary to find new alternatives to treat the disease. In this study, phage UPM2146 was isolated from a polluted lake which can lyse its host K. pneumoniae ATCC BAA-2146. Observation from TEM shows that UPM2146 belongs to Caudoviriales (Order) based on morphological appearance. Whole genome analysis of UPM2146 showed that its genome comprises 160,795 bp encoding for 214 putative open reading frames (ORFs). Phylogenetic analysis revealed that the phage belongs to Ackermannviridae (Family) under the Caudoviriales. UPM2146 produces clear plaques with high titers of 1010 PFU/ml. The phage has an adsorption period of 4 min, latent period of 20 min, rise period of 5 min, and releases approximately 20 PFU/ bacteria at Multiplicity of Infection (MOI) of 0.001. UPM2146 has a narrow host-range and can lyse 5 out of 22 K. pneumoniae isolates (22.72%) based on spot test and efficiency of plating (EOP). The zebrafish larvae model was used to test the efficacy of UPM2146 in lysing its host. Based on colony forming unit counts, UPM2146 was able to completely lyse its host at 10 hours onwards. Moreover, we show that the phage is safe to be used in the treatment against K. pneumoniae infections in the zebrafish model. |
| first_indexed | 2025-11-15T13:08:19Z |
| format | Article |
| id | upm-93983 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:08:19Z |
| publishDate | 2021 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-939832023-04-12T04:24:34Z http://psasir.upm.edu.my/id/eprint/93983/ Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo Assafiri, Omar Song, Adelene Ai Lian Tan, Geok Hun Hanish, Irwan Mohd Hashim, Amalia Yusoff, Khatijah Klebsiella pneumoniae are opportunistic bacteria found in the gut. In recent years they have been associated with nosocomial infections. The increased incidence of multiple drug-resistant K. pneumoniae makes it necessary to find new alternatives to treat the disease. In this study, phage UPM2146 was isolated from a polluted lake which can lyse its host K. pneumoniae ATCC BAA-2146. Observation from TEM shows that UPM2146 belongs to Caudoviriales (Order) based on morphological appearance. Whole genome analysis of UPM2146 showed that its genome comprises 160,795 bp encoding for 214 putative open reading frames (ORFs). Phylogenetic analysis revealed that the phage belongs to Ackermannviridae (Family) under the Caudoviriales. UPM2146 produces clear plaques with high titers of 1010 PFU/ml. The phage has an adsorption period of 4 min, latent period of 20 min, rise period of 5 min, and releases approximately 20 PFU/ bacteria at Multiplicity of Infection (MOI) of 0.001. UPM2146 has a narrow host-range and can lyse 5 out of 22 K. pneumoniae isolates (22.72%) based on spot test and efficiency of plating (EOP). The zebrafish larvae model was used to test the efficacy of UPM2146 in lysing its host. Based on colony forming unit counts, UPM2146 was able to completely lyse its host at 10 hours onwards. Moreover, we show that the phage is safe to be used in the treatment against K. pneumoniae infections in the zebrafish model. Public Library of Science 2021-01-08 Article PeerReviewed Assafiri, Omar and Song, Adelene Ai Lian and Tan, Geok Hun and Hanish, Irwan and Mohd Hashim, Amalia and Yusoff, Khatijah (2021) Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo. PLoS One, 16 (1). pp. 1-19. ISSN 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245354#abstract0 10.1371/journal.pone.0245354 |
| spellingShingle | Assafiri, Omar Song, Adelene Ai Lian Tan, Geok Hun Hanish, Irwan Mohd Hashim, Amalia Yusoff, Khatijah Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo |
| title | Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo |
| title_full | Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo |
| title_fullStr | Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo |
| title_full_unstemmed | Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo |
| title_short | Klebsiella virus UPM2146 lyses multiple drugresistant Klebsiella pneumoniae in vitro and in vivo |
| title_sort | klebsiella virus upm2146 lyses multiple drugresistant klebsiella pneumoniae in vitro and in vivo |
| url | http://psasir.upm.edu.my/id/eprint/93983/ http://psasir.upm.edu.my/id/eprint/93983/ http://psasir.upm.edu.my/id/eprint/93983/ |