Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats
This study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of Pasteurella multocida B:2 following intranasal vaccination against hemorrhagic septicemia in goats. Goats were vaccinated intranasal with 10...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Springer Science + Business Media B.V. 2007
2010
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/9368/ http://psasir.upm.edu.my/id/eprint/9368/1/Efficacy%20of%20an%20inactivated%20recombinant%20vaccine%20encoding%20a%20fimbrial%20protein%20of%20Pasteurella%20multocida%20B.pdf |
| _version_ | 1848841127175651328 |
|---|---|
| author | Mohd Yasin, Ina Salwany Mohd Yusoff, Sabri Saad, Mohd Zamri Abd Wahid, Mohd Effendy |
| author_facet | Mohd Yasin, Ina Salwany Mohd Yusoff, Sabri Saad, Mohd Zamri Abd Wahid, Mohd Effendy |
| author_sort | Mohd Yasin, Ina Salwany |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | This study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of Pasteurella multocida B:2 following intranasal vaccination against hemorrhagic septicemia in goats. Goats were vaccinated intranasal with 106 CFU/mL of the recombinant vaccine (vaccinated group) and 106 CFU/mL of pET32/LIC vector without fimbrial protein (control group). All three groups were kept separated before all goats in the three groups were challenged with 109 CFU/mL of live pathogenic P. multocida B:2. During the course of study, both serum and lung lavage fluid were collected to evaluate the antibody levels via enzyme-linked immunosorbent assay. It was found that goats immunized with the inactivated recombinant vaccine developed a strong and significantly (p < 0.05) higher specific IgA and IgG responses in both serum and lung lavage fluid samples compared to the control and unvaccinated groups. Following intratracheal challenge, the rate of isolation was 17% for the vaccinated group, 67% for the control group and 100% for the unvaccinated group. However, none of the goat from the vaccinated group had P. multocida B:2 in the liver, tonsil and heart. Therefore, the study revealed that an inactivated recombinant vaccine significantly provides significant protection against high dose challenge and enhances the stimulation of the local and systemic immunities.
|
| first_indexed | 2025-11-15T07:38:18Z |
| format | Article |
| id | upm-9368 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:38:18Z |
| publishDate | 2010 |
| publisher | Springer Science + Business Media B.V. 2007 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-93682015-10-20T08:06:23Z http://psasir.upm.edu.my/id/eprint/9368/ Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats Mohd Yasin, Ina Salwany Mohd Yusoff, Sabri Saad, Mohd Zamri Abd Wahid, Mohd Effendy This study was carried out to determine the antibody responses and protective capacity of an inactivated recombinant vaccine expressing the fimbrial protein of Pasteurella multocida B:2 following intranasal vaccination against hemorrhagic septicemia in goats. Goats were vaccinated intranasal with 106 CFU/mL of the recombinant vaccine (vaccinated group) and 106 CFU/mL of pET32/LIC vector without fimbrial protein (control group). All three groups were kept separated before all goats in the three groups were challenged with 109 CFU/mL of live pathogenic P. multocida B:2. During the course of study, both serum and lung lavage fluid were collected to evaluate the antibody levels via enzyme-linked immunosorbent assay. It was found that goats immunized with the inactivated recombinant vaccine developed a strong and significantly (p < 0.05) higher specific IgA and IgG responses in both serum and lung lavage fluid samples compared to the control and unvaccinated groups. Following intratracheal challenge, the rate of isolation was 17% for the vaccinated group, 67% for the control group and 100% for the unvaccinated group. However, none of the goat from the vaccinated group had P. multocida B:2 in the liver, tonsil and heart. Therefore, the study revealed that an inactivated recombinant vaccine significantly provides significant protection against high dose challenge and enhances the stimulation of the local and systemic immunities. Springer Science + Business Media B.V. 2007 2010-08-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/9368/1/Efficacy%20of%20an%20inactivated%20recombinant%20vaccine%20encoding%20a%20fimbrial%20protein%20of%20Pasteurella%20multocida%20B.pdf Mohd Yasin, Ina Salwany and Mohd Yusoff, Sabri and Saad, Mohd Zamri and Abd Wahid, Mohd Effendy (2010) Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats. Tropical Animal Health and Production, 43 (1). pp. 179-187. ISSN 0049-4747 http://dx.doi.org/10.1007/s11250-010-9672-5 10.1007/s11250-010-9672-5 English |
| spellingShingle | Mohd Yasin, Ina Salwany Mohd Yusoff, Sabri Saad, Mohd Zamri Abd Wahid, Mohd Effendy Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats |
| title | Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats |
| title_full | Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats |
| title_fullStr | Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats |
| title_full_unstemmed | Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats |
| title_short | Efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of Pasteurella multocida B:2 against hemorrhagic septicemia in goats |
| title_sort | efficacy of an inactivated recombinant vaccine encoding a fimbrial protein of pasteurella multocida b:2 against hemorrhagic septicemia in goats |
| url | http://psasir.upm.edu.my/id/eprint/9368/ http://psasir.upm.edu.my/id/eprint/9368/ http://psasir.upm.edu.my/id/eprint/9368/ http://psasir.upm.edu.my/id/eprint/9368/1/Efficacy%20of%20an%20inactivated%20recombinant%20vaccine%20encoding%20a%20fimbrial%20protein%20of%20Pasteurella%20multocida%20B.pdf |