Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation
A new cell line, Asian sea bass brain (ASBB), was derived from the brain tissue of Asian sea bass Lates calcarifer. This cell line was maintained in Leibovitz L-15 media supplemented with 10% fetal bovine serum (FBS). The ASBB cell line was subcultured more than 60 times over a period of 15 mo. The...
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| Format: | Article |
| Language: | English English |
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The Society for In Vitro Biology 2010
2010
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| Online Access: | http://psasir.upm.edu.my/id/eprint/9364/ http://psasir.upm.edu.my/id/eprint/9364/1/Development%20and%20partial%20characterization%20of%20new%20marine%20cell%20line%20from%20brain%20of%20Asian%20sea%20bass%20Lates%20calcarifer%20for%20virus%20isolation.pdf |
| _version_ | 1848841126101909504 |
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| author | F. Hasoon, Mauida Mohd Daud, Hassan A. Abdullah, Azila Arshad, Siti Suri Bejo, Mohd Hair |
| author_facet | F. Hasoon, Mauida Mohd Daud, Hassan A. Abdullah, Azila Arshad, Siti Suri Bejo, Mohd Hair |
| author_sort | F. Hasoon, Mauida |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A new cell line, Asian sea bass brain (ASBB), was derived from the brain tissue of Asian sea bass Lates calcarifer. This cell line was maintained in Leibovitz L-15 media supplemented with 10% fetal bovine serum (FBS). The ASBB cell line was subcultured more than 60 times over a period of 15 mo. The ASBB cell line consists predominantly of fibroblastic-like cells and was able to grow at temperatures between 20°C and 30°C with an optimum temperature of 25°C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 25°C with optimum growth at the concentrations of 10% or 15% FBS. Polymerase chain reaction products were obtained from ASBB cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. An isolate of piscine nodavirus from juveniles of marine fish species tested positive by IQ2000 kit for viral nervous necrosis detection and was examined for its infectivity to a fish cell line of ASBB. A marine fish betanodavirus was tested to determine the susceptibility of this new cell line in comparison with commercial highly permissive SSN-1 cells. The ASBB cell line was found to be susceptible to nodavirus (RGNNV genotype), and the infection was confirmed by comparison cytopathic effect (CPE) with commercial SSN-1 and reverse transcriptase-polymerase chain reaction. A nodavirus was further elucidated by electron microscopy, and the virus tested was shown to induce CPE on ASBB cells with significant high titer. This suggests that the ASBB cell line has good potential for the isolation of fish viruses.
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| first_indexed | 2025-11-15T07:38:17Z |
| format | Article |
| id | upm-9364 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T07:38:17Z |
| publishDate | 2010 |
| publisher | The Society for In Vitro Biology 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-93642015-09-22T03:34:58Z http://psasir.upm.edu.my/id/eprint/9364/ Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation F. Hasoon, Mauida Mohd Daud, Hassan A. Abdullah, Azila Arshad, Siti Suri Bejo, Mohd Hair A new cell line, Asian sea bass brain (ASBB), was derived from the brain tissue of Asian sea bass Lates calcarifer. This cell line was maintained in Leibovitz L-15 media supplemented with 10% fetal bovine serum (FBS). The ASBB cell line was subcultured more than 60 times over a period of 15 mo. The ASBB cell line consists predominantly of fibroblastic-like cells and was able to grow at temperatures between 20°C and 30°C with an optimum temperature of 25°C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 25°C with optimum growth at the concentrations of 10% or 15% FBS. Polymerase chain reaction products were obtained from ASBB cells and tissues of sea bass with primer sets of microsatellite markers of sea bass. An isolate of piscine nodavirus from juveniles of marine fish species tested positive by IQ2000 kit for viral nervous necrosis detection and was examined for its infectivity to a fish cell line of ASBB. A marine fish betanodavirus was tested to determine the susceptibility of this new cell line in comparison with commercial highly permissive SSN-1 cells. The ASBB cell line was found to be susceptible to nodavirus (RGNNV genotype), and the infection was confirmed by comparison cytopathic effect (CPE) with commercial SSN-1 and reverse transcriptase-polymerase chain reaction. A nodavirus was further elucidated by electron microscopy, and the virus tested was shown to induce CPE on ASBB cells with significant high titer. This suggests that the ASBB cell line has good potential for the isolation of fish viruses. The Society for In Vitro Biology 2010 2010-11-22 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/9364/1/Development%20and%20partial%20characterization%20of%20new%20marine%20cell%20line%20from%20brain%20of%20Asian%20sea%20bass%20Lates%20calcarifer%20for%20virus%20isolation.pdf F. Hasoon, Mauida and Mohd Daud, Hassan and A. Abdullah, Azila and Arshad, Siti Suri and Bejo, Mohd Hair (2010) Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation. In Vitro Cellular & Developmental Biology - Animal, 47 (1). pp. 1-10. ISSN 1071-2690 http://dx.doi.org/10.1007/s11626-010-9348-5 10.1007/s11626-010-9348-5 English |
| spellingShingle | F. Hasoon, Mauida Mohd Daud, Hassan A. Abdullah, Azila Arshad, Siti Suri Bejo, Mohd Hair Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation |
| title | Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation |
| title_full | Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation |
| title_fullStr | Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation |
| title_full_unstemmed | Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation |
| title_short | Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation |
| title_sort | development and partial characterization of new marine cell line from brain of asian sea bass lates calcarifer for virus isolation |
| url | http://psasir.upm.edu.my/id/eprint/9364/ http://psasir.upm.edu.my/id/eprint/9364/ http://psasir.upm.edu.my/id/eprint/9364/ http://psasir.upm.edu.my/id/eprint/9364/1/Development%20and%20partial%20characterization%20of%20new%20marine%20cell%20line%20from%20brain%20of%20Asian%20sea%20bass%20Lates%20calcarifer%20for%20virus%20isolation.pdf |