Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment

This study investigated the natural incidence of deoxynivalenol (DON) in corn and corn products from corn-producing districts of Punjab, Pakistan. The analysis was carried out using high performance liquid chromatography (HPLC) with UV detector and immunoaffinity cleanup columns. The detection limit...

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Main Authors: Iqbal, Shahzad Zafar, Abdull Razis, Ahmad Faizal, Usman, Sunusi, Ali, Nada Basheir, Asi, Muhammad Rafique
Format: Article
Language:English
Published: MDPI AG 2021
Online Access:http://psasir.upm.edu.my/id/eprint/93536/
http://psasir.upm.edu.my/id/eprint/93536/1/93536.pdf
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author Iqbal, Shahzad Zafar
Abdull Razis, Ahmad Faizal
Usman, Sunusi
Ali, Nada Basheir
Asi, Muhammad Rafique
author_facet Iqbal, Shahzad Zafar
Abdull Razis, Ahmad Faizal
Usman, Sunusi
Ali, Nada Basheir
Asi, Muhammad Rafique
author_sort Iqbal, Shahzad Zafar
building UPM Institutional Repository
collection Online Access
description This study investigated the natural incidence of deoxynivalenol (DON) in corn and corn products from corn-producing districts of Punjab, Pakistan. The analysis was carried out using high performance liquid chromatography (HPLC) with UV detector and immunoaffinity cleanup columns. The detection limit (LOD) and limit of quantification were 25 and 50 µg/kg, respectively. A total of 1220 samples of corn and corn products were analyzed to detect DON, and 539 (44.2%) samples were observed to be contaminated with DON (n ≥ LOD). Furthermore, 92 (7.5%) samples of corn and corn products had DON levels that were higher than the proposed limits of the EU. The data are significantly different from a normal distribution of DON in samples of corn and corn products from different locations (p < 0.05) for Shapiro–Wilk and Kolmogorov–Smirnov values. However, a significant difference in DON levels was found between corn and corn-derived products (p ≤ 0.05). The lowest and highest exposures, and hazard quotient (HQ) values of 0.92 and 9.68 µg/kg bw/day, were documented in corn flour samples.
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spelling upm-935362025-02-10T06:34:31Z http://psasir.upm.edu.my/id/eprint/93536/ Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment Iqbal, Shahzad Zafar Abdull Razis, Ahmad Faizal Usman, Sunusi Ali, Nada Basheir Asi, Muhammad Rafique This study investigated the natural incidence of deoxynivalenol (DON) in corn and corn products from corn-producing districts of Punjab, Pakistan. The analysis was carried out using high performance liquid chromatography (HPLC) with UV detector and immunoaffinity cleanup columns. The detection limit (LOD) and limit of quantification were 25 and 50 µg/kg, respectively. A total of 1220 samples of corn and corn products were analyzed to detect DON, and 539 (44.2%) samples were observed to be contaminated with DON (n ≥ LOD). Furthermore, 92 (7.5%) samples of corn and corn products had DON levels that were higher than the proposed limits of the EU. The data are significantly different from a normal distribution of DON in samples of corn and corn products from different locations (p < 0.05) for Shapiro–Wilk and Kolmogorov–Smirnov values. However, a significant difference in DON levels was found between corn and corn-derived products (p ≤ 0.05). The lowest and highest exposures, and hazard quotient (HQ) values of 0.92 and 9.68 µg/kg bw/day, were documented in corn flour samples. MDPI AG 2021-04 Article PeerReviewed text en cc_by_4 http://psasir.upm.edu.my/id/eprint/93536/1/93536.pdf Iqbal, Shahzad Zafar and Abdull Razis, Ahmad Faizal and Usman, Sunusi and Ali, Nada Basheir and Asi, Muhammad Rafique (2021) Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment. Toxins, 13 (5). art. no. 296. ISSN 2072-6651; eISSN: 2072-6651 https://www.mdpi.com/2072-6651/13/5/296 10.3390/toxins13050296
spellingShingle Iqbal, Shahzad Zafar
Abdull Razis, Ahmad Faizal
Usman, Sunusi
Ali, Nada Basheir
Asi, Muhammad Rafique
Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment
title Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment
title_full Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment
title_fullStr Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment
title_full_unstemmed Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment
title_short Variation of deoxynivalenol levels in corn and its products available in retail markets of Punjab, Pakistan and estimation of risk assessment
title_sort variation of deoxynivalenol levels in corn and its products available in retail markets of punjab, pakistan and estimation of risk assessment
url http://psasir.upm.edu.my/id/eprint/93536/
http://psasir.upm.edu.my/id/eprint/93536/
http://psasir.upm.edu.my/id/eprint/93536/
http://psasir.upm.edu.my/id/eprint/93536/1/93536.pdf