Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies
The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canin...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
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Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://psasir.upm.edu.my/id/eprint/93442/ |
| _version_ | 1848861846721789952 |
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| author | Tan, Wei Miao Seng, Fong Lau Syed Sulaiman, Syarifah Zakiah Umran, Nor Shahira Solehah Ajat, Mokrish Mansor, Rozaihan Megat Abdul Rani, Puteri Azaziah Shafie, Intan Nurfatiha Ng, Angela Min Hwei Rahmad, Norasfaliza |
| author_facet | Tan, Wei Miao Seng, Fong Lau Syed Sulaiman, Syarifah Zakiah Umran, Nor Shahira Solehah Ajat, Mokrish Mansor, Rozaihan Megat Abdul Rani, Puteri Azaziah Shafie, Intan Nurfatiha Ng, Angela Min Hwei Rahmad, Norasfaliza |
| author_sort | Tan, Wei Miao |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canine synovial fluid for proteome studies. Pooled synovial fluid samples were obtained from clinically healthy dogs (n=5), with no radiographic features of osteoarthritis. The acetone precipitation method and 10% w/v of trichloroacetic acid/acetone were chosen to deplete the albumin from canine synovial fluid and the outcome from the different methods were compared using one dimensional and twodimensional gel electrophoresis studies. The results showed that the 10% w/v TCA/acetone precipitation method removed highly abundant proteins from synovial fluid for gel electrophoresis studies compared to the acetone precipitation method. The 10% w/v TCA/acetone precipitation method provides an effective method to remove albumin from the synovial fluid using gel electrophoresis, especially two-dimensional gel electrophoresis. The accessibility and cost of TCA and acetone make this method of precipitation a simple and cost-effective technique in preparing a canine synovial fluid for two-dimensional gel electrophoresis analysis. |
| first_indexed | 2025-11-15T13:07:37Z |
| format | Article |
| id | upm-93442 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T13:07:37Z |
| publishDate | 2021 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-934422023-01-12T06:49:19Z http://psasir.upm.edu.my/id/eprint/93442/ Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies Tan, Wei Miao Seng, Fong Lau Syed Sulaiman, Syarifah Zakiah Umran, Nor Shahira Solehah Ajat, Mokrish Mansor, Rozaihan Megat Abdul Rani, Puteri Azaziah Shafie, Intan Nurfatiha Ng, Angela Min Hwei Rahmad, Norasfaliza The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is the optimization step, which involves the depletion of high abundant proteins from the samples. The objective of this study was to develop a cost efficient and effective method to remove albumin from canine synovial fluid for proteome studies. Pooled synovial fluid samples were obtained from clinically healthy dogs (n=5), with no radiographic features of osteoarthritis. The acetone precipitation method and 10% w/v of trichloroacetic acid/acetone were chosen to deplete the albumin from canine synovial fluid and the outcome from the different methods were compared using one dimensional and twodimensional gel electrophoresis studies. The results showed that the 10% w/v TCA/acetone precipitation method removed highly abundant proteins from synovial fluid for gel electrophoresis studies compared to the acetone precipitation method. The 10% w/v TCA/acetone precipitation method provides an effective method to remove albumin from the synovial fluid using gel electrophoresis, especially two-dimensional gel electrophoresis. The accessibility and cost of TCA and acetone make this method of precipitation a simple and cost-effective technique in preparing a canine synovial fluid for two-dimensional gel electrophoresis analysis. Universiti Kebangsaan Malaysia 2021-11 Article PeerReviewed Tan, Wei Miao and Seng, Fong Lau and Syed Sulaiman, Syarifah Zakiah and Umran, Nor Shahira Solehah and Ajat, Mokrish and Mansor, Rozaihan and Megat Abdul Rani, Puteri Azaziah and Shafie, Intan Nurfatiha and Ng, Angela Min Hwei and Rahmad, Norasfaliza (2021) Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies. Sains Malaysiana, 50 (11). pp. 3297-3302. ISSN 0126-6039; ESSN: 2735-0118 https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/Jilid50Bil11_2021ms3297-3302.html |
| spellingShingle | Tan, Wei Miao Seng, Fong Lau Syed Sulaiman, Syarifah Zakiah Umran, Nor Shahira Solehah Ajat, Mokrish Mansor, Rozaihan Megat Abdul Rani, Puteri Azaziah Shafie, Intan Nurfatiha Ng, Angela Min Hwei Rahmad, Norasfaliza Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_full | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_fullStr | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_full_unstemmed | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_short | Trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| title_sort | trichloroacetic acid/acetone precipitation method to optimize canine synovial fluid for one and two-dimensional gel electrophoresis studies |
| url | http://psasir.upm.edu.my/id/eprint/93442/ http://psasir.upm.edu.my/id/eprint/93442/ |