Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent

Endophytic lactic acid bacteria (LAB) isolated from papaya seeds, including a consortium of two LAB isolates, Weissella cibaria PPKSD19 and Lactococcus lactis subsp. lactis PPSSD39 could previously inhibit papaya dieback disease causative agent, Erwinia mallotivora BT-MARDI in vitro, indicating the...

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Main Authors: Mohd Jaini, Mohammad Fahrulazri, Roslan, Nurfaten Farhanah, Yusof, Mohd Termizi, Saidi, Noor Baity, Ramli, Norhayati, Mohd Zainudin, Nur Ain Izzati, Mohd Hashim, Amalia
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2022
Online Access:http://psasir.upm.edu.my/id/eprint/92541/
http://psasir.upm.edu.my/id/eprint/92541/1/12%20JTAS-2379-2021.pdf
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author Mohd Jaini, Mohammad Fahrulazri
Roslan, Nurfaten Farhanah
Yusof, Mohd Termizi
Saidi, Noor Baity
Ramli, Norhayati
Mohd Zainudin, Nur Ain Izzati
Mohd Hashim, Amalia
author_facet Mohd Jaini, Mohammad Fahrulazri
Roslan, Nurfaten Farhanah
Yusof, Mohd Termizi
Saidi, Noor Baity
Ramli, Norhayati
Mohd Zainudin, Nur Ain Izzati
Mohd Hashim, Amalia
author_sort Mohd Jaini, Mohammad Fahrulazri
building UPM Institutional Repository
collection Online Access
description Endophytic lactic acid bacteria (LAB) isolated from papaya seeds, including a consortium of two LAB isolates, Weissella cibaria PPKSD19 and Lactococcus lactis subsp. lactis PPSSD39 could previously inhibit papaya dieback disease causative agent, Erwinia mallotivora BT-MARDI in vitro, indicating their potential as biofertilizer. However, further characterizations on other plant growth-promoting (PGP) properties of the LABs are prerequisite to use in agricultural settings as bio-inoculum. Hence, this study aimed to evaluate PGP potentials further and in vitro antifungal activity of the LABs against various plant pathogens. The LAB isolates were tested positive in indole-3-acetic acid (IAA), siderophore, and ammonia production and could solubilize phosphate. Weissella cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 exhibited the strongest in vitro antifungal activity against Fusarium oxysporum TKA and Curvularia lunata. Inoculum concentration of 1x10 cfu/ml of W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 mixture showed the highest increment in shoot and root dry weight. In conclusion, W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 consortium displayed promising plant probiotic potential. These results highlighted the possibility of the bacterial consortium to be exploited as bioinoculant to promote plant growth and inhibit phytopathogens causing plant diseases.
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spelling upm-925412022-05-27T08:42:48Z http://psasir.upm.edu.my/id/eprint/92541/ Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent Mohd Jaini, Mohammad Fahrulazri Roslan, Nurfaten Farhanah Yusof, Mohd Termizi Saidi, Noor Baity Ramli, Norhayati Mohd Zainudin, Nur Ain Izzati Mohd Hashim, Amalia Endophytic lactic acid bacteria (LAB) isolated from papaya seeds, including a consortium of two LAB isolates, Weissella cibaria PPKSD19 and Lactococcus lactis subsp. lactis PPSSD39 could previously inhibit papaya dieback disease causative agent, Erwinia mallotivora BT-MARDI in vitro, indicating their potential as biofertilizer. However, further characterizations on other plant growth-promoting (PGP) properties of the LABs are prerequisite to use in agricultural settings as bio-inoculum. Hence, this study aimed to evaluate PGP potentials further and in vitro antifungal activity of the LABs against various plant pathogens. The LAB isolates were tested positive in indole-3-acetic acid (IAA), siderophore, and ammonia production and could solubilize phosphate. Weissella cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 exhibited the strongest in vitro antifungal activity against Fusarium oxysporum TKA and Curvularia lunata. Inoculum concentration of 1x10 cfu/ml of W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 mixture showed the highest increment in shoot and root dry weight. In conclusion, W. cibaria PPKSD19 and L. lactis subsp. lactis PPSSD39 consortium displayed promising plant probiotic potential. These results highlighted the possibility of the bacterial consortium to be exploited as bioinoculant to promote plant growth and inhibit phytopathogens causing plant diseases. Universiti Putra Malaysia Press 2022 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/92541/1/12%20JTAS-2379-2021.pdf Mohd Jaini, Mohammad Fahrulazri and Roslan, Nurfaten Farhanah and Yusof, Mohd Termizi and Saidi, Noor Baity and Ramli, Norhayati and Mohd Zainudin, Nur Ain Izzati and Mohd Hashim, Amalia (2022) Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent. Pertanika Journal of Tropical Agricultural Science, 45 (1). pp. 207-233. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/pjtas/browse/regular-issue?article=JTAS-2379-2021 10.47836/pjtas.45.1.12
spellingShingle Mohd Jaini, Mohammad Fahrulazri
Roslan, Nurfaten Farhanah
Yusof, Mohd Termizi
Saidi, Noor Baity
Ramli, Norhayati
Mohd Zainudin, Nur Ain Izzati
Mohd Hashim, Amalia
Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
title Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
title_full Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
title_fullStr Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
title_full_unstemmed Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
title_short Investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
title_sort investigating the potential of endophytic lactic acid bacteria isolated from papaya seeds as plant growth promoter and antifungal agent
url http://psasir.upm.edu.my/id/eprint/92541/
http://psasir.upm.edu.my/id/eprint/92541/
http://psasir.upm.edu.my/id/eprint/92541/
http://psasir.upm.edu.my/id/eprint/92541/1/12%20JTAS-2379-2021.pdf