In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments
The present study was conducted to establish in vitro regeneration responses of callus of Catharanthus roseus to chitosan treatments in combination withPGR (IBA+NAA). Leaf petioles from 5 weeks old plants were used as explant callus initiated was cultured on half MS (Murashige and Skoog, 1974) me...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/91585/ http://psasir.upm.edu.my/id/eprint/91585/1/FP%202015%2083%20-%20IR.pdf |
| _version_ | 1848861394246565888 |
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| author | Gandoh, Norhafizah |
| author_facet | Gandoh, Norhafizah |
| author_sort | Gandoh, Norhafizah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study was conducted to establish in vitro regeneration responses
of callus of Catharanthus roseus to chitosan treatments in combination withPGR
(IBA+NAA). Leaf petioles from 5 weeks old plants were used as explant callus
initiated was cultured on half MS (Murashige and Skoog, 1974) media containing
PGR and different concentrations of chitosan. The callus was cultured in medium
supplementedwith (0, 1, 2, 3, 4 and 5 mg/L) chitosan in 16 treatments combination of
IBA and NAA each with(0, 1.5, 3.0, and 4.5 mg/L). The parameters used to access
growth and development of callus was number of roots, length of roots and dry
weight of callus. The experiment was conducted in a complete randomized design
(CRD) in 3 replications. Medium containing 5 mg/L chitosan shows high dry weight
of with 0.786 g and treatment with 2 mg/L of chitosan gavethe highest number and
longest length of roots with 53.66 and 2.47 cm respectively. The study shows
positive effects of chitosan on C. roseus. |
| first_indexed | 2025-11-15T13:00:26Z |
| format | Project Paper Report |
| id | upm-91585 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T13:00:26Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-915852021-12-01T04:06:19Z http://psasir.upm.edu.my/id/eprint/91585/ In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments Gandoh, Norhafizah The present study was conducted to establish in vitro regeneration responses of callus of Catharanthus roseus to chitosan treatments in combination withPGR (IBA+NAA). Leaf petioles from 5 weeks old plants were used as explant callus initiated was cultured on half MS (Murashige and Skoog, 1974) media containing PGR and different concentrations of chitosan. The callus was cultured in medium supplementedwith (0, 1, 2, 3, 4 and 5 mg/L) chitosan in 16 treatments combination of IBA and NAA each with(0, 1.5, 3.0, and 4.5 mg/L). The parameters used to access growth and development of callus was number of roots, length of roots and dry weight of callus. The experiment was conducted in a complete randomized design (CRD) in 3 replications. Medium containing 5 mg/L chitosan shows high dry weight of with 0.786 g and treatment with 2 mg/L of chitosan gavethe highest number and longest length of roots with 53.66 and 2.47 cm respectively. The study shows positive effects of chitosan on C. roseus. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91585/1/FP%202015%2083%20-%20IR.pdf Gandoh, Norhafizah (2015) In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments. [Project Paper Report] |
| spellingShingle | Gandoh, Norhafizah In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments |
| title | In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments |
| title_full | In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments |
| title_fullStr | In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments |
| title_full_unstemmed | In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments |
| title_short | In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments |
| title_sort | in vitro regeneration responses of callus of catharanthus roseus (l) g.don. to plant growth regulators and chitosan treatments |
| url | http://psasir.upm.edu.my/id/eprint/91585/ http://psasir.upm.edu.my/id/eprint/91585/1/FP%202015%2083%20-%20IR.pdf |