In vitro regeneration responses of Callus of Catharanthus roseus (L) G.Don. to plant growth regulators and chitosan treatments
The present study was conducted to establish in vitro regeneration responses of callus of Catharanthus roseus to chitosan treatments in combination withPGR (IBA+NAA). Leaf petioles from 5 weeks old plants were used as explant callus initiated was cultured on half MS (Murashige and Skoog, 1974) me...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/91585/ http://psasir.upm.edu.my/id/eprint/91585/1/FP%202015%2083%20-%20IR.pdf |
| Summary: | The present study was conducted to establish in vitro regeneration responses
of callus of Catharanthus roseus to chitosan treatments in combination withPGR
(IBA+NAA). Leaf petioles from 5 weeks old plants were used as explant callus
initiated was cultured on half MS (Murashige and Skoog, 1974) media containing
PGR and different concentrations of chitosan. The callus was cultured in medium
supplementedwith (0, 1, 2, 3, 4 and 5 mg/L) chitosan in 16 treatments combination of
IBA and NAA each with(0, 1.5, 3.0, and 4.5 mg/L). The parameters used to access
growth and development of callus was number of roots, length of roots and dry
weight of callus. The experiment was conducted in a complete randomized design
(CRD) in 3 replications. Medium containing 5 mg/L chitosan shows high dry weight
of with 0.786 g and treatment with 2 mg/L of chitosan gavethe highest number and
longest length of roots with 53.66 and 2.47 cm respectively. The study shows
positive effects of chitosan on C. roseus. |
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