Screening of antimicrobial activity of bacteria from natural habitats to control Xanthomonas campestris pv. campestris in cabbage
The black rot disease is a serious disease on Brassica oleracea caused by Xanthomonas campestris pv. campestris. The majority of cabbages were affected by black rot disease and caused economic losses in Malaysia. The problem I am trying to solve in this study is to use potential bacteria culture...
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/91476/ http://psasir.upm.edu.my/id/eprint/91476/1/FP%202017%2078%20-%20IR.pdf |
| Summary: | The black rot disease is a serious disease on Brassica oleracea caused by
Xanthomonas campestris pv. campestris. The majority of cabbages were affected by
black rot disease and caused economic losses in Malaysia. The problem I am trying to
solve in this study is to use potential bacteria culture to inhibit the Xanthomonas
campestris pv. campestris growth. The approach which I adopted is antimicrobial
susceptibility test (AST). A total of 106 bacteria samples were used for AST. Each
sample was repeated twice. Result showed that 32 bacteria have the potential to
control Xanthomonas campestris pv. campestris in vitro. The data were analyzed with
ANOVA and mean separations of LSD tests using SAS 9.4. Sample No.12, No.15, No.
24 and No.28 showed bigger size inhibition areas as compared to control. These four
samples were also characterized for gram staining and identified using 16s rRNA
molecular method. From identification result, No.12 was identified as Bacillus cereus;
No.15 as Brevibacillus parabrevis. Bacteria No.24 and No.28 were identified as
Bacillus subtilis. |
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