Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L.
Mango (Mangifera indica L.) belongs to the family Anacardiaceae is grown primarily in Malaysia valued for local mango production and has high nutritional value. One of the major problem pre and post-harvest diseases on mango is anthracnose disease caused by many fungal species in the genus Collet...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/91166/ http://psasir.upm.edu.my/id/eprint/91166/1/FP%202016%2060%20-%20IR.pdf |
| _version_ | 1848861272522620928 |
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| author | Mohd Din, Siti Norliza |
| author_facet | Mohd Din, Siti Norliza |
| author_sort | Mohd Din, Siti Norliza |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Mango (Mangifera indica L.) belongs to the family Anacardiaceae is grown
primarily in Malaysia valued for local mango production and has high nutritional value.
One of the major problem pre and post-harvest diseases on mango is anthracnose disease
caused by many fungal species in the genus Colletotrichum. Symptoms of the disease
included irregular, circular dark brown spot appear on the young leaves, flowers and
fruits. In Malaysia, there is limited research on the composition of fungal species
responsible for mango anthracnose. The objectives of this study are; 1) to isolate pure
culture of fungal isolates causing anthracnose on mango fruits and leaves; 2) to identify
fungal pathogens to species level based on morphological characteristics and polymerase
chain reaction (PCR) protocol using ITS4 and ITS5 primers; and 3) to construct internal
transcribed spacer (ITS) phylogeny of the fungal species using maximum likelihood
analysis. To accomplish these objectives, symptomatic fruits were collected from five
different mango trees at Taman Pertanian Universiti (TPU), Universiti Putra Malaysia.
Infected tissues (5 x 5mm) from the lesions margin was being surface disinfected for 2
min with 10% Clorox and cultured on potato dextrose agar (PDA). The pure fungal
isolates isolated from fruit lesions were identified by conidial and in vitro morphological
characteristics according to Mordue et al.,(1971). The fungal isolates were sub-cultured
by single spore isolation and the representative was characterized further. DNA genomic
was extracted from fresh fungal mycelium by using protocol of DNeasy Plant Mini Kit
from QIAGEN. The internal transcribed spacer (ITS) region of the ribosomal DNA was
amplified using primers ITS4 and ITS5. The PCR product of the ITS was sequenced and
analyzed using BLAST nucleotide query in GenBank. In this study, all fungal isolates match to the sequence of Colletotrichum asianum within C.gloeosporiodes species
complex. This study is a significant step forward management recommendation in
controlling anthracnose in mango production areas. |
| first_indexed | 2025-11-15T12:58:30Z |
| format | Project Paper Report |
| id | upm-91166 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:58:30Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-911662021-11-03T02:43:00Z http://psasir.upm.edu.my/id/eprint/91166/ Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. Mohd Din, Siti Norliza Mango (Mangifera indica L.) belongs to the family Anacardiaceae is grown primarily in Malaysia valued for local mango production and has high nutritional value. One of the major problem pre and post-harvest diseases on mango is anthracnose disease caused by many fungal species in the genus Colletotrichum. Symptoms of the disease included irregular, circular dark brown spot appear on the young leaves, flowers and fruits. In Malaysia, there is limited research on the composition of fungal species responsible for mango anthracnose. The objectives of this study are; 1) to isolate pure culture of fungal isolates causing anthracnose on mango fruits and leaves; 2) to identify fungal pathogens to species level based on morphological characteristics and polymerase chain reaction (PCR) protocol using ITS4 and ITS5 primers; and 3) to construct internal transcribed spacer (ITS) phylogeny of the fungal species using maximum likelihood analysis. To accomplish these objectives, symptomatic fruits were collected from five different mango trees at Taman Pertanian Universiti (TPU), Universiti Putra Malaysia. Infected tissues (5 x 5mm) from the lesions margin was being surface disinfected for 2 min with 10% Clorox and cultured on potato dextrose agar (PDA). The pure fungal isolates isolated from fruit lesions were identified by conidial and in vitro morphological characteristics according to Mordue et al.,(1971). The fungal isolates were sub-cultured by single spore isolation and the representative was characterized further. DNA genomic was extracted from fresh fungal mycelium by using protocol of DNeasy Plant Mini Kit from QIAGEN. The internal transcribed spacer (ITS) region of the ribosomal DNA was amplified using primers ITS4 and ITS5. The PCR product of the ITS was sequenced and analyzed using BLAST nucleotide query in GenBank. In this study, all fungal isolates match to the sequence of Colletotrichum asianum within C.gloeosporiodes species complex. This study is a significant step forward management recommendation in controlling anthracnose in mango production areas. 2016 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91166/1/FP%202016%2060%20-%20IR.pdf Mohd Din, Siti Norliza (2016) Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. [Project Paper Report] |
| spellingShingle | Mohd Din, Siti Norliza Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. |
| title | Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. |
| title_full | Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. |
| title_fullStr | Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. |
| title_full_unstemmed | Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. |
| title_short | Identification and characterization of fungal species causing anthracnose disease on mango (Mangifera indica L. |
| title_sort | identification and characterization of fungal species causing anthracnose disease on mango (mangifera indica l. |
| url | http://psasir.upm.edu.my/id/eprint/91166/ http://psasir.upm.edu.my/id/eprint/91166/1/FP%202016%2060%20-%20IR.pdf |