Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines
Erlotinib, a small-molecule epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, has been shown to be an effective solution in treating non-small cell lung cancer (NSCLC). However, most patients who initially show a good response to erlotinib treatment eventually develop resistant and...
| Main Author: | |
|---|---|
| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/91013/ http://psasir.upm.edu.my/id/eprint/91013/1/FBSB%202015%20132%20-%20IR.pdf |
| _version_ | 1848861228243353600 |
|---|---|
| author | Mat Daud, Syukriyah |
| author_facet | Mat Daud, Syukriyah |
| author_sort | Mat Daud, Syukriyah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Erlotinib, a small-molecule epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, has been shown to be an effective solution in treating non-small cell lung cancer (NSCLC). However, most patients who initially show a good response to erlotinib treatment eventually develop resistant and experience progressive disease. There is no absolute answer on how the cells can develop resistance against the erlotinib. It was suggested that changes in the cell cycle could contribute to the acquired resistance of NSCLC. In this study, untreated H1299 and erlotinib-resistant H1299 (H1299-R) were used to compare proteins expression levels involved in cell cycle. The main cell cycle players that were investigated included Cyclin A and E, Cyclin-dependent kinases (CDK) 2, 4, and 6, p21, NFKB, and ß-actin. A calourimetric method, alkaline phosphatase, was used to detect the amount of proteins present in the sample. Although the concentration of the protein loaded into the gel was increased from 75 μg to 85 μg, the results obtained were similar. Both cell lines possessed ß-actin and Cyclin A proteins only. |
| first_indexed | 2025-11-15T12:57:47Z |
| format | Project Paper Report |
| id | upm-91013 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:57:47Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-910132021-10-18T01:35:06Z http://psasir.upm.edu.my/id/eprint/91013/ Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines Mat Daud, Syukriyah Erlotinib, a small-molecule epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor, has been shown to be an effective solution in treating non-small cell lung cancer (NSCLC). However, most patients who initially show a good response to erlotinib treatment eventually develop resistant and experience progressive disease. There is no absolute answer on how the cells can develop resistance against the erlotinib. It was suggested that changes in the cell cycle could contribute to the acquired resistance of NSCLC. In this study, untreated H1299 and erlotinib-resistant H1299 (H1299-R) were used to compare proteins expression levels involved in cell cycle. The main cell cycle players that were investigated included Cyclin A and E, Cyclin-dependent kinases (CDK) 2, 4, and 6, p21, NFKB, and ß-actin. A calourimetric method, alkaline phosphatase, was used to detect the amount of proteins present in the sample. Although the concentration of the protein loaded into the gel was increased from 75 μg to 85 μg, the results obtained were similar. Both cell lines possessed ß-actin and Cyclin A proteins only. 2015 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/91013/1/FBSB%202015%20132%20-%20IR.pdf Mat Daud, Syukriyah (2015) Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines. [Project Paper Report] |
| spellingShingle | Mat Daud, Syukriyah Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines |
| title | Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines |
| title_full | Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines |
| title_fullStr | Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines |
| title_full_unstemmed | Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines |
| title_short | Identification of deregulation in protein expression level of erlotinib-resistant H1299 cell lines |
| title_sort | identification of deregulation in protein expression level of erlotinib-resistant h1299 cell lines |
| url | http://psasir.upm.edu.my/id/eprint/91013/ http://psasir.upm.edu.my/id/eprint/91013/1/FBSB%202015%20132%20-%20IR.pdf |