Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii
Vanadium-dependent haloperoxidases (VHPOs) belong to one of the three classes of haloperoxidases which can oxidize halogens including chloride (Cl-), bromide (Br-), and iodide (I-). Chemical halogenation of organic compounds typically requires harsh conditions. Hence, biohalogenation has gained i...
| Main Author: | |
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| Format: | Project Paper Report |
| Language: | English |
| Published: |
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/90989/ http://psasir.upm.edu.my/id/eprint/90989/1/FBSB%202015%20167%20-%20IR.pdf |
| _version_ | 1848861221384617984 |
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| author | Ng, Shaunie May Qi |
| author_facet | Ng, Shaunie May Qi |
| author_sort | Ng, Shaunie May Qi |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vanadium-dependent haloperoxidases (VHPOs) belong to one of the three classes of
haloperoxidases which can oxidize halogens including chloride (Cl-), bromide (Br-),
and iodide (I-). Chemical halogenation of organic compounds typically requires harsh
conditions. Hence, biohalogenation has gained increasing research interest. As
vanadium-dependent haloperoxidases are enzymes that catalyze halogenation of
organic compounds, they are very valuable due to their potential applications in
various industries as well as their stability and tolerance for different conditions. This
study aims to optimize the recombinant protein expression conditions, for potentially large scale production of vanadium-dependent bromoperoxidase 2 (GcVBPO2) that
was previously isolated from Gracilaria changii. GcVBPO2 sequence in pET32a(+)
was transformed into expression host Escherichia coli BL21(DE3) pLySs and induced
at various temperatures. GcVBPO2 was found to be soluble when induced with
0.5mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 16 hours in Luria-Bertani
(LB) broth culture at 20°C. Purification of GcVBPO2 was performed using His-tag
purification, and subsequently analysed with Western blot. |
| first_indexed | 2025-11-15T12:57:41Z |
| format | Project Paper Report |
| id | upm-90989 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:57:41Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-909892021-10-13T03:03:29Z http://psasir.upm.edu.my/id/eprint/90989/ Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii Ng, Shaunie May Qi Vanadium-dependent haloperoxidases (VHPOs) belong to one of the three classes of haloperoxidases which can oxidize halogens including chloride (Cl-), bromide (Br-), and iodide (I-). Chemical halogenation of organic compounds typically requires harsh conditions. Hence, biohalogenation has gained increasing research interest. As vanadium-dependent haloperoxidases are enzymes that catalyze halogenation of organic compounds, they are very valuable due to their potential applications in various industries as well as their stability and tolerance for different conditions. This study aims to optimize the recombinant protein expression conditions, for potentially large scale production of vanadium-dependent bromoperoxidase 2 (GcVBPO2) that was previously isolated from Gracilaria changii. GcVBPO2 sequence in pET32a(+) was transformed into expression host Escherichia coli BL21(DE3) pLySs and induced at various temperatures. GcVBPO2 was found to be soluble when induced with 0.5mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 16 hours in Luria-Bertani (LB) broth culture at 20°C. Purification of GcVBPO2 was performed using His-tag purification, and subsequently analysed with Western blot. 2015-06 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/90989/1/FBSB%202015%20167%20-%20IR.pdf Ng, Shaunie May Qi (2015) Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii. [Project Paper Report] |
| spellingShingle | Ng, Shaunie May Qi Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| title | Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| title_full | Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| title_fullStr | Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| title_full_unstemmed | Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| title_short | Optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| title_sort | optimization of recombinant vanadium-dependent haloperoxidase production from gracilaria changii |
| url | http://psasir.upm.edu.my/id/eprint/90989/ http://psasir.upm.edu.my/id/eprint/90989/1/FBSB%202015%20167%20-%20IR.pdf |