Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah

Introduction: The outbreaks of foodborne diseases have been linked to the consumption of contaminated seafood. This research aims to screen the bacteria from the sea cucumbers Acaudina molpadioides collected from Pulau Langkawi. Methods: A total of 22 sea cucumber samples were collected randomly fro...

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Main Authors: Li, Abigail Yen Lew, Mazlan, Nurzafirah, Anua, Siti Marwanis, Thung, Tze Young
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2020
Online Access:http://psasir.upm.edu.my/id/eprint/90229/
http://psasir.upm.edu.my/id/eprint/90229/1/7upm.pdf
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author Li, Abigail Yen Lew
Mazlan, Nurzafirah
Anua, Siti Marwanis
Thung, Tze Young
author_facet Li, Abigail Yen Lew
Mazlan, Nurzafirah
Anua, Siti Marwanis
Thung, Tze Young
author_sort Li, Abigail Yen Lew
building UPM Institutional Repository
collection Online Access
description Introduction: The outbreaks of foodborne diseases have been linked to the consumption of contaminated seafood. This research aims to screen the bacteria from the sea cucumbers Acaudina molpadioides collected from Pulau Langkawi. Methods: A total of 22 sea cucumber samples were collected randomly from Pulau Langkawi, Kedah, Malaysia. The samples were isolated and identified for the presence of bacteria using the conventional culture-based method. Presumptive bacteria colonies were subjected to various biochemical and antimicrobial susceptibility tests. Results: There were no bacterial growth in Hektoen Enteric (HE) agar and Thiosulphate-Citrate-Bile Salt (TCBS) agar. Positive samples were isolated from MacConkey (MAC) agar with 6 samples were Staphylococcus spp. (27.27%), 14 samples were Proteus spp. (63.63%) and 2 samples were Bacillus spp. (9.01%). Among these isolates, highest resistance was found against Ampicillin (45%) followed by Tetracycline (40%). Conclusion: The results indicate that the sea cucumbers Acaudina molpadioides were contaminated with potential bacteria. There is a need for adequate consumer protection measures.
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spelling upm-902292021-09-09T21:51:43Z http://psasir.upm.edu.my/id/eprint/90229/ Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah Li, Abigail Yen Lew Mazlan, Nurzafirah Anua, Siti Marwanis Thung, Tze Young Introduction: The outbreaks of foodborne diseases have been linked to the consumption of contaminated seafood. This research aims to screen the bacteria from the sea cucumbers Acaudina molpadioides collected from Pulau Langkawi. Methods: A total of 22 sea cucumber samples were collected randomly from Pulau Langkawi, Kedah, Malaysia. The samples were isolated and identified for the presence of bacteria using the conventional culture-based method. Presumptive bacteria colonies were subjected to various biochemical and antimicrobial susceptibility tests. Results: There were no bacterial growth in Hektoen Enteric (HE) agar and Thiosulphate-Citrate-Bile Salt (TCBS) agar. Positive samples were isolated from MacConkey (MAC) agar with 6 samples were Staphylococcus spp. (27.27%), 14 samples were Proteus spp. (63.63%) and 2 samples were Bacillus spp. (9.01%). Among these isolates, highest resistance was found against Ampicillin (45%) followed by Tetracycline (40%). Conclusion: The results indicate that the sea cucumbers Acaudina molpadioides were contaminated with potential bacteria. There is a need for adequate consumer protection measures. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2020-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/90229/1/7upm.pdf Li, Abigail Yen Lew and Mazlan, Nurzafirah and Anua, Siti Marwanis and Thung, Tze Young (2020) Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah. Malaysian Journal of Medicine and Health Sciences, 16 (3). pp. 42-46. ISSN 1675-8544; ESSN: 2636-9346 https://medic.upm.edu.my/upload/dokumen/2020090809024007_MJMHS_0083.pdf
spellingShingle Li, Abigail Yen Lew
Mazlan, Nurzafirah
Anua, Siti Marwanis
Thung, Tze Young
Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah
title Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah
title_full Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah
title_fullStr Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah
title_full_unstemmed Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah
title_short Screening of pathogenic bacteria from sea cucumber Acaudina molpadioides in Pulau Langkawi, Kedah
title_sort screening of pathogenic bacteria from sea cucumber acaudina molpadioides in pulau langkawi, kedah
url http://psasir.upm.edu.my/id/eprint/90229/
http://psasir.upm.edu.my/id/eprint/90229/
http://psasir.upm.edu.my/id/eprint/90229/1/7upm.pdf