Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura

Plant essential oil play important role as one of the biopesticide in controlling insect pest. Lemon oil extract have the potential to be used as biopesticide to against insect pest of cruciferous crop. Larvae of Spodoptera litura are known to cause enormous losses to many important cultivated crops...

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Main Author: Rahim, Naufal Fahmi
Format: Project Paper Report
Language:English
Published: 2018
Online Access:http://psasir.upm.edu.my/id/eprint/89743/
http://psasir.upm.edu.my/id/eprint/89743/1/FP%202018%2029%20IR.pdf
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author Rahim, Naufal Fahmi
author_facet Rahim, Naufal Fahmi
author_sort Rahim, Naufal Fahmi
building UPM Institutional Repository
collection Online Access
description Plant essential oil play important role as one of the biopesticide in controlling insect pest. Lemon oil extract have the potential to be used as biopesticide to against insect pest of cruciferous crop. Larvae of Spodoptera litura are known to cause enormous losses to many important cultivated crops. Thus the objective of this study is to evaluate the larvicidal activity of lemon extract against S. litura. Experiment was conducted at Toxicology laboratory, Department of Plant Protection Faculty of Agriculture, UPM Serdang. Larvicidal activity of S.litura larvae was determined using leaf dipping method and feeding deterrence index. The larvae mortality, leaf area and dry weight of feaces were recorded at 24, 48, 72 and 96 hours after treatment. The treatment concentration for both lemon crude extract (LCE) and lemon oil extract (LOE) were 0.01%, 0.05%, 0.1%, 1%, 2%, Spinosad as the positive control and negative control was distilled water. The mortality data was subjected to Probit Analysis to determine the toxicity level by the LC50 value. Results showed that LC50 value at 96 hours exposure was 237.958 ml/L for lemon crude extract and lemon oil extract was 496.412 ml/L. The antifeedant showed the rate increases when the concentration and exposure time increased. All concentration gave 100% antifeedant rate at 96 hours. The lowest antifeedant was 1.87% at 0.01% LOE at 24 hours. LCE and LOE showed positive result for controlling S.litura as well as environmental friendly.
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institution Universiti Putra Malaysia
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spelling upm-897432021-06-21T06:13:02Z http://psasir.upm.edu.my/id/eprint/89743/ Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura Rahim, Naufal Fahmi Plant essential oil play important role as one of the biopesticide in controlling insect pest. Lemon oil extract have the potential to be used as biopesticide to against insect pest of cruciferous crop. Larvae of Spodoptera litura are known to cause enormous losses to many important cultivated crops. Thus the objective of this study is to evaluate the larvicidal activity of lemon extract against S. litura. Experiment was conducted at Toxicology laboratory, Department of Plant Protection Faculty of Agriculture, UPM Serdang. Larvicidal activity of S.litura larvae was determined using leaf dipping method and feeding deterrence index. The larvae mortality, leaf area and dry weight of feaces were recorded at 24, 48, 72 and 96 hours after treatment. The treatment concentration for both lemon crude extract (LCE) and lemon oil extract (LOE) were 0.01%, 0.05%, 0.1%, 1%, 2%, Spinosad as the positive control and negative control was distilled water. The mortality data was subjected to Probit Analysis to determine the toxicity level by the LC50 value. Results showed that LC50 value at 96 hours exposure was 237.958 ml/L for lemon crude extract and lemon oil extract was 496.412 ml/L. The antifeedant showed the rate increases when the concentration and exposure time increased. All concentration gave 100% antifeedant rate at 96 hours. The lowest antifeedant was 1.87% at 0.01% LOE at 24 hours. LCE and LOE showed positive result for controlling S.litura as well as environmental friendly. 2018 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/89743/1/FP%202018%2029%20IR.pdf Rahim, Naufal Fahmi (2018) Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura. [Project Paper Report]
spellingShingle Rahim, Naufal Fahmi
Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura
title Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura
title_full Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura
title_fullStr Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura
title_full_unstemmed Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura
title_short Larvicidal activity of lemon oil and lemon crude extract against Spodoptera litura
title_sort larvicidal activity of lemon oil and lemon crude extract against spodoptera litura
url http://psasir.upm.edu.my/id/eprint/89743/
http://psasir.upm.edu.my/id/eprint/89743/1/FP%202018%2029%20IR.pdf