Isolation of citric acid-producing Aspergillus niger from soil and organic wastes
Introduction of new Aspergillus niger strains that are more productive than those currently in use is one of the important steps in promoting more effective commercial citric acid production. The present study was conducted to isolate and characterise indigenous A. niger from selected soil and organ...
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| Format: | Article |
| Language: | English |
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Update Publishing House
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89483/ http://psasir.upm.edu.my/id/eprint/89483/1/CITRIC.pdf |
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| author | Ab Aziz, Zakry Fitri Nurul Shaffee Syahidah Malahubban, Masnindah Show, Pau Loke |
| author_facet | Ab Aziz, Zakry Fitri Nurul Shaffee Syahidah Malahubban, Masnindah Show, Pau Loke |
| author_sort | Ab Aziz, Zakry Fitri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Introduction of new Aspergillus niger strains that are more productive than those currently in use is one of the important steps in promoting more effective commercial citric acid production. The present study was conducted to isolate and characterise indigenous A. niger from selected soil and organic wastes such as soil with buried bamboo, soil with bamboo on the surface, soil under cattle grazing, mango orchard soil, rotting plum fruit and rotting bread. Morphological identification of A. niger was based on the length and width of the conidiophores, vesicles, phialides, and spores. Citric acid-producing Aspergillus isolates were screened based on the citric acid production index. The present study found that the highest fungal spore counts (3.35±0.15 x 107 spores/g sample) were obtained from soil under cattle grazing, as were the highest A. niger counts (7.25±0.05 x 106 spores/g sample). The lowest total fungal counts came from rotting plum fruit (4.70±0.10 x 105 spores/g sample). A total of 14 isolates were collected, with five (NSA03, NSA06, NSA09, NSA12, and NSA14) showing morphological similarities with the reference isolate, A. niger Tiegh. All isolates were able to produce citric acid, but with varying efficiencies according to their citric acid production indices. The soil under cattle grazing area found to be the best site for sampling and isolation for citric acid-producing A. niger by using Czapek-Dox as a medium of isolation. |
| first_indexed | 2025-11-15T12:52:00Z |
| format | Article |
| id | upm-89483 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:52:00Z |
| publishDate | 2020 |
| publisher | Update Publishing House |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-894832021-08-16T11:42:06Z http://psasir.upm.edu.my/id/eprint/89483/ Isolation of citric acid-producing Aspergillus niger from soil and organic wastes Ab Aziz, Zakry Fitri Nurul Shaffee Syahidah Malahubban, Masnindah Show, Pau Loke Introduction of new Aspergillus niger strains that are more productive than those currently in use is one of the important steps in promoting more effective commercial citric acid production. The present study was conducted to isolate and characterise indigenous A. niger from selected soil and organic wastes such as soil with buried bamboo, soil with bamboo on the surface, soil under cattle grazing, mango orchard soil, rotting plum fruit and rotting bread. Morphological identification of A. niger was based on the length and width of the conidiophores, vesicles, phialides, and spores. Citric acid-producing Aspergillus isolates were screened based on the citric acid production index. The present study found that the highest fungal spore counts (3.35±0.15 x 107 spores/g sample) were obtained from soil under cattle grazing, as were the highest A. niger counts (7.25±0.05 x 106 spores/g sample). The lowest total fungal counts came from rotting plum fruit (4.70±0.10 x 105 spores/g sample). A total of 14 isolates were collected, with five (NSA03, NSA06, NSA09, NSA12, and NSA14) showing morphological similarities with the reference isolate, A. niger Tiegh. All isolates were able to produce citric acid, but with varying efficiencies according to their citric acid production indices. The soil under cattle grazing area found to be the best site for sampling and isolation for citric acid-producing A. niger by using Czapek-Dox as a medium of isolation. Update Publishing House 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89483/1/CITRIC.pdf Ab Aziz, Zakry Fitri and Nurul Shaffee Syahidah and Malahubban, Masnindah and Show, Pau Loke (2020) Isolation of citric acid-producing Aspergillus niger from soil and organic wastes. Journal of Phytology, 12. 104 - 108. ISSN 2075-6240 https://updatepublishing.com/journal/index.php/jp/article/view/6423 10.25081/jp.2020.v12.6423 |
| spellingShingle | Ab Aziz, Zakry Fitri Nurul Shaffee Syahidah Malahubban, Masnindah Show, Pau Loke Isolation of citric acid-producing Aspergillus niger from soil and organic wastes |
| title | Isolation of citric acid-producing Aspergillus niger from soil and organic wastes |
| title_full | Isolation of citric acid-producing Aspergillus niger from soil and organic wastes |
| title_fullStr | Isolation of citric acid-producing Aspergillus niger from soil and organic wastes |
| title_full_unstemmed | Isolation of citric acid-producing Aspergillus niger from soil and organic wastes |
| title_short | Isolation of citric acid-producing Aspergillus niger from soil and organic wastes |
| title_sort | isolation of citric acid-producing aspergillus niger from soil and organic wastes |
| url | http://psasir.upm.edu.my/id/eprint/89483/ http://psasir.upm.edu.my/id/eprint/89483/ http://psasir.upm.edu.my/id/eprint/89483/ http://psasir.upm.edu.my/id/eprint/89483/1/CITRIC.pdf |