Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions
The occurrences of multiple drug-resistant strains have been relentlessly increasing in recent years. The aquaculture industry has encountered major disease outbreaks and crucially affected by this situation. The usage of non-specific chemicals and antibiotics expedites the stimulation of resistant...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89397/ http://psasir.upm.edu.my/id/eprint/89397/1/SERINE.pdf |
| _version_ | 1848860844372262912 |
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| author | Ravichandran, Gayathri Pasupuleti, Mukesh Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Arockiaraj, Jesu |
| author_facet | Ravichandran, Gayathri Pasupuleti, Mukesh Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Arockiaraj, Jesu |
| author_sort | Ravichandran, Gayathri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The occurrences of multiple drug-resistant strains have been relentlessly increasing in recent years. The aquaculture industry has encountered major disease outbreaks and crucially affected by this situation. The usage of non-specific chemicals and antibiotics expedites the stimulation of resistant strains. Triggering the natural defense mechanism would provide an effective and safest way of protecting the host system. Hence, we have investigated the innate immune function of serine/threonine-protein kinase (STPK) in Macrobrachium rosenbergii (Mr). The in-silico protein analysis resulted in the identification of cationic antimicrobial peptide, MrSL-19, with interesting properties from STPK of M. rosenbergii. Antimicrobial assay, FACS and SEM analysis demonstrated that the peptide potentially inhibits Staphylococcus aureus by interacting with its membrane. The toxic study on MrSL-19 demonstrated that the peptide is not toxic against HEK293 cells as well as human erythrocytes. This investigation showed the significant innate immune property of an efficient cationic antimicrobial peptide, MrSL-19 of STPK from M. rosenbergii. |
| first_indexed | 2025-11-15T12:51:41Z |
| format | Article |
| id | upm-89397 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:41Z |
| publishDate | 2020 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-893972021-08-19T22:59:28Z http://psasir.upm.edu.my/id/eprint/89397/ Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions Ravichandran, Gayathri Pasupuleti, Mukesh Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Arockiaraj, Jesu The occurrences of multiple drug-resistant strains have been relentlessly increasing in recent years. The aquaculture industry has encountered major disease outbreaks and crucially affected by this situation. The usage of non-specific chemicals and antibiotics expedites the stimulation of resistant strains. Triggering the natural defense mechanism would provide an effective and safest way of protecting the host system. Hence, we have investigated the innate immune function of serine/threonine-protein kinase (STPK) in Macrobrachium rosenbergii (Mr). The in-silico protein analysis resulted in the identification of cationic antimicrobial peptide, MrSL-19, with interesting properties from STPK of M. rosenbergii. Antimicrobial assay, FACS and SEM analysis demonstrated that the peptide potentially inhibits Staphylococcus aureus by interacting with its membrane. The toxic study on MrSL-19 demonstrated that the peptide is not toxic against HEK293 cells as well as human erythrocytes. This investigation showed the significant innate immune property of an efficient cationic antimicrobial peptide, MrSL-19 of STPK from M. rosenbergii. Elsevier 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89397/1/SERINE.pdf Ravichandran, Gayathri and Pasupuleti, Mukesh and Arasu, Mariadhas Valan and Al-Dhabi, Naif Abdullah and Arshad, Aziz and Arockiaraj, Jesu (2020) Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions. Fish and Shellfish Immunology, 106. 332 - 340. ISSN 1050-4648; ESSN: 1095-9947 https://www.sciencedirect.com/science/article/pii/S1050464820305520 10.1016/j.fsi.2020.07.068 |
| spellingShingle | Ravichandran, Gayathri Pasupuleti, Mukesh Arasu, Mariadhas Valan Al-Dhabi, Naif Abdullah Arshad, Aziz Arockiaraj, Jesu Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions |
| title | Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions |
| title_full | Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions |
| title_fullStr | Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions |
| title_full_unstemmed | Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions |
| title_short | Innate immune function of serine/threonine-protein kinase from Macrobrachium rosenbergii in response to host-pathogen interactions |
| title_sort | innate immune function of serine/threonine-protein kinase from macrobrachium rosenbergii in response to host-pathogen interactions |
| url | http://psasir.upm.edu.my/id/eprint/89397/ http://psasir.upm.edu.my/id/eprint/89397/ http://psasir.upm.edu.my/id/eprint/89397/ http://psasir.upm.edu.my/id/eprint/89397/1/SERINE.pdf |