Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant
Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Me...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020
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| Online Access: | http://psasir.upm.edu.my/id/eprint/89348/ http://psasir.upm.edu.my/id/eprint/89348/1/CALLUS.pdf |
| _version_ | 1848860832557957120 |
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| author | Kamarul Zaman, Munirah Adibah Mohamad Azzeme, Azzreena Ramle, Illy Kamaliah Normanshah, Nurfazlinyana Ramli, Siti Nurhafizah Shaharuddin, Noor Azmi Ahmad, Syahida Abdullah, Siti Nor Akmar |
| author_facet | Kamarul Zaman, Munirah Adibah Mohamad Azzeme, Azzreena Ramle, Illy Kamaliah Normanshah, Nurfazlinyana Ramli, Siti Nurhafizah Shaharuddin, Noor Azmi Ahmad, Syahida Abdullah, Siti Nor Akmar |
| author_sort | Kamarul Zaman, Munirah Adibah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production. |
| first_indexed | 2025-11-15T12:51:30Z |
| format | Article |
| id | upm-89348 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T12:51:30Z |
| publishDate | 2020 |
| publisher | Multidisciplinary Digital Publishing Institute |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-893482021-09-03T05:44:29Z http://psasir.upm.edu.my/id/eprint/89348/ Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant Kamarul Zaman, Munirah Adibah Mohamad Azzeme, Azzreena Ramle, Illy Kamaliah Normanshah, Nurfazlinyana Ramli, Siti Nurhafizah Shaharuddin, Noor Azmi Ahmad, Syahida Abdullah, Siti Nor Akmar Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production. Multidisciplinary Digital Publishing Institute 2020 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/89348/1/CALLUS.pdf Kamarul Zaman, Munirah Adibah and Mohamad Azzeme, Azzreena and Ramle, Illy Kamaliah and Normanshah, Nurfazlinyana and Ramli, Siti Nurhafizah and Shaharuddin, Noor Azmi and Ahmad, Syahida and Abdullah, Siti Nor Akmar (2020) Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant. Plants-Basel, 9 (12). pp. 1-23. ISSN 2223-7747 https://www.mdpi.com/2223-7747/9/12/1772 10.3390/plants9121772 |
| spellingShingle | Kamarul Zaman, Munirah Adibah Mohamad Azzeme, Azzreena Ramle, Illy Kamaliah Normanshah, Nurfazlinyana Ramli, Siti Nurhafizah Shaharuddin, Noor Azmi Ahmad, Syahida Abdullah, Siti Nor Akmar Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant |
| title | Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant |
| title_full | Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant |
| title_fullStr | Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant |
| title_full_unstemmed | Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant |
| title_short | Induction, multiplication, and evaluation of antioxidant activity of Polyalthia bullata callus, a woody medicinal plant |
| title_sort | induction, multiplication, and evaluation of antioxidant activity of polyalthia bullata callus, a woody medicinal plant |
| url | http://psasir.upm.edu.my/id/eprint/89348/ http://psasir.upm.edu.my/id/eprint/89348/ http://psasir.upm.edu.my/id/eprint/89348/ http://psasir.upm.edu.my/id/eprint/89348/1/CALLUS.pdf |